Configurations of the optimization mode and imaging mode of the high-speed adaptive LSFM. (a) Schematic (top view) of the optimization mode. (b) The actual orientation of O1 and LED viewed end on. (c) Schematic (section view) of the DM flattening setup. (d) Schematic (top view) of the imaging mode including illumination and imaging parts. (e) The actual orientation of O1 and O2 viewed end on. O, objective; TL, tube lens; M, mirror; L, lens; LD, Lambertian diffuser; STM, star test mask; DM, deformable mirror; HLS, halogen light source; BS, beam splitter; SHWS, Shack–Hartmann wavefront sensor; GM, galvo mirror; and EF, emission filter.