The deformability of human red blood cells (RBCs), which comprise almost 99% of the cells in whole blood, is largely related not only to pathophysiological blood flow but also to the levels of intracellular compounds. Therefore, statistical estimates of the deformability of individual RBCs are of paramount importance in the clinical diagnosis of blood diseases. Although the microscale hydrodynamic interactions of individual RBCs lead to non-Newtonian blood rheology, there is no established method to estimate individual RBC deformability from the rheological data of RBC suspensions, and the possibility of this estimation has not been proven. To address this issue, we conducted an integrated analysis of a model of the rheology of RBC suspensions, coupled with macrorheological data of human RBCs suspended in plasma. Assuming a nonlinear curve of the relative viscosity of the suspensions as a function of the cell volume fraction, the statistical average of the membrane shear elasticity was estimated for individual intact RBCs or hardened RBCs. Both estimated values reproduced well the experimentally observed shear-thinning non-Newtonian behavior in these suspensions. We hereby conclude that our complementary approach makes it possible to estimate the statistical average of individual RBC deformability from macrorheological data obtained with usual rheometric tests.
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November 2024
Research Article|
September 24 2024
Numerical-experimental estimation of the deformability of human red blood cells from rheometrical data
Naoki Takeishi
;
Naoki Takeishi
a)
1
Department of Mechanical Engineering, Kyushu University
, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan
a)Author to whom correspondence should be addressed; electronic mail: takeishi.naoki.008@m.kyushu-u.ac.jp
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Tomohiro Nishiyama
;
Tomohiro Nishiyama
2
Department of Pure and Applied Physics, Kansai University
, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan
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Kodai Nagaishi;
Kodai Nagaishi
2
Department of Pure and Applied Physics, Kansai University
, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan
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Takeshi Nashima;
Takeshi Nashima
3
OhnaTech Inc.
, 2-1-6 Sengen, Tsukuba, Ibraki 305-0047, Japan
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Masako Sugihara-Seki
Masako Sugihara-Seki
2
Department of Pure and Applied Physics, Kansai University
, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan
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a)Author to whom correspondence should be addressed; electronic mail: takeishi.naoki.008@m.kyushu-u.ac.jp
J. Rheol. 68, 837–848 (2024)
Article history
Received:
May 11 2024
Accepted:
September 09 2024
Connected Content
A companion article has been published:
Key blood disease indicator can be estimated with rheological blood test data
Citation
Naoki Takeishi, Tomohiro Nishiyama, Kodai Nagaishi, Takeshi Nashima, Masako Sugihara-Seki; Numerical-experimental estimation of the deformability of human red blood cells from rheometrical data. J. Rheol. 1 November 2024; 68 (6): 837–848. https://doi.org/10.1122/8.0000877
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