How the bacterium Pseudomonas syringae induces water to crystallize

Ice-nucleating bacteria can catalyze ice formation in supercooled water stored in plant tissue. That ability enables them to rupture plant cell walls and release the nutrients the plants contain. Bacterial catalysis also provokes water in clouds to freeze; ice-nucleating bacteria may thus have a significant impact on weather and climate. Invoking molecular simulations, scientists conjectured that the surface of a bacterial protein organizes the liquid near it to promote crystallization. That hypothesis has now received experimental confirmation in work led by Tobias Weidner at the Max Planck Institute for Polymer Research. Weidner and colleagues studied the bacterium Pseudomonas syringae, whose ice-nucleating protein is called inaZ. They used sum-frequency generation (SFG) spectroscopy, in which IR and visible laser pulses aimed at an interface return a strong signal only if the interface is populated with ordered molecules. The observed signal increased as the researchers reduced the temperature, a manifestation of inaZ’s...