The effects of light emission diode (LED) light exposure on retinal pigment epithelial (RPE) cells were examined to clarify the mechanisms of cellular damage or change caused by LED. The RPE cells were isolated from bovine eyes and exposed to the LED light. After exposure, cell proliferation, the lactate dehydrogenase (LDH) activity in medium, 4-hydroxy-2-nonenal (HNE) and apoptosis were determined as indicators of oxidative stress and cell injury. Also, we determined reactive oxygen species (ROS) which mediated the tissue injury in RPE cells after LED light exposure. Wavelengths of 460 nm, 620 nm or 880 nm were chosen for the examinations. Cell proliferation decreased by 24% and 15% of control by 50 J/cm2 of 460 nm LED and 620 nm LED exposure, respectively, although no change was observed at 100 J/cm2 of 880 nm LED exposure. LDH activity in the medium was increased to 50% above control by 10 J/cm2 460 nm LED light but did not increase after 620 nm LED light exposure. ROS generation was increased to 73% and 35% above control by 10 J/cm2 of 460 nm and 620 run LED exposure, respectively. After 24 hr at 10 J/cm2 of 460 nm exposure, RPE cells showed a positive staining for HNE. After 72 hr, apoptosis was not observed by 10 J/cm2 of 460 nm (1/300) and 620 nm (1/501). These results indicate that photochemical damage to RPE cells occurred at 10 J/cm2 of 460 nm and 620 nm LED light exposure, suggesting in part, mediation by mitochondrial free radical signaling and oxidative stress after LED light exposure.
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International Laser Safety Conference
November 27–30, 1990
Cincinnati, Ohio, USA
PROCEEDINGS PAPER
Effect of Light-Emitting Diode (LED) Light Exposure on Retinal Pigment Epithelial cells, In Vitro
Takako N. Ueda;
Takako N. Ueda
1
Department of Pharmacology, School of Medicine, Showa University
, 1-5-8, Hatanodai, Shinagawa-ku, Tokyo
, 142-8555
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Shohei Fukuda;
Shohei Fukuda
2
Departments of Ophthalmology, School of Medicine, Showa University
, 1-5-8, Hatanodai, Shinagawa, Tokyo, 142-8666, Japan
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Toshihiko Ueda;
Toshihiko Ueda
2
Departments of Ophthalmology, School of Medicine, Showa University
, 1-5-8, Hatanodai, Shinagawa, Tokyo, 142-8666, Japan
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Tetsuma Ozawa;
Tetsuma Ozawa
2
Departments of Ophthalmology, School of Medicine, Showa University
, 1-5-8, Hatanodai, Shinagawa, Tokyo, 142-8666, Japan
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Ryohei Koide;
Ryohei Koide
2
Departments of Ophthalmology, School of Medicine, Showa University
, 1-5-8, Hatanodai, Shinagawa, Tokyo, 142-8666, Japan
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Hideyuki J. Majima
Hideyuki J. Majima
3
Space and Particle Radiation Science Research Group, National Institute of Radiological Sciences
, 4-9-1, Anagawa, Inage, Chiba, 263-8555, Japan
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Published Online:
March 01 1999
Citation
Takako N. Ueda, Shohei Fukuda, Toshihiko Ueda, Tetsuma Ozawa, Ryohei Koide, Hideyuki J. Majima; November 27–30, 1990. "Effect of Light-Emitting Diode (LED) Light Exposure on Retinal Pigment Epithelial cells, In Vitro." Proceedings of the International Laser Safety Conference. ILSC® ‘99: Proceedings of the International Laser Safety Conference - Volume 4b. Cincinnati, Ohio, USA. (pp. pp. 57-62). ASME. https://doi.org/10.2351/1.5056484
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