The present study focused on the environmentally friendly preparation, characterization, and certain biological activities of gold nanoparticles (Au-NPs) made with the Gundelia tournefortii (kenger) leaf extract. The results from devices such as transmission electron microscope, energy-dispersive x-ray spectrophotometer, and Fourier scanning electron microscope reveal the structures of nanoparticles. After an hour, the greatest surface plasmon resonance was discovered at 532.15 nm. The mean cubic crystallite size was calculated using the powder x-ray diffraction model and was found to be 23.53 nm. It was noted that the produced Au-NPs had spherical forms and hexagonal dimensions and ranged in size from 5 to 40 nm. The minimum inhibitory concentration and a colorimetric method for determining cell metabolic activity test, respectively, were used to investigate the suppressive effects of Au-NPs on the growth of pathogenic bacteria and healthy and cancer cell lines.

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