Corneal damage thresholds were determined for exposures to 3 ns single pulse emitted at 1.57μm, using clinical observations and histology. The DE50 obtained with comeal beam diameter of 400μm was 26.6 J.cm−2. The corresponding radiant exposure calculated with the 1mm aperture diameter recommended by ICNIRP guidelines or standards, was 4.3 J.cm−2. This value was still upper than the present 1 J.cm−2 exposure limit. In vitro experiments have been also carried out on primary keratocytes and epithelial cell line. The infrared 1.57μm laser radiation can induce morphological nucleic abnormalities. The frequency of apoptotic bodies seemed the most suitable criteria capable to reveal the cellular stress induced by laser irradiation.
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© 2007 Laser Institute of America.
2007
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