Ship-in-a-bottle integration by hybrid femtosecond laser processing for fabrication of highly functional biochips

Here we propose a novel strategy which fuses femtoseond laser-assisted etching of glass (FLAE) and femtosecond laser two photon polymerization (TPP) to integrate 3D complex polymer microdevices into glass-based microfluidic devices for highly functional applications. A series of polymer microstructures from 1D nanolines to 2D controllable micropatterns to 3D complex microcages were integrated in 3D glass microchannels. Such distinct microchips have been termed ship-in-a-bottle biochips. The high quality of microchips was ensured by quantitatively investigating the experimental processes and optimizing the parameters. The ship-in-a-bottle biochips show high capabilities to provide simultaneous filtering and mixing with 87% efficiency in a shorter distance and on-chip synthesis of ZnO microflower particles.