Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence microscopy but it is also a powerful 3D microfabrication technique for manufacturing of submicron structures and patterns of biological molecules. TPP provides an efficient way to pattern surfaces of cell culture dishes or tissue engineering scaffolds and to guide the growth of cultivated cells. In this study the TPP process was utilized to crosslink two biologically relevant proteins – avidin and bovine serum albumin. The polymerization setup included a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was operated at 532 nm. Submicron protein strings were fabricated by moving a motorized X-Y-Z stage at a suitable rate along a predefined path. The width of the protein patterns could be varied by changing the laser power and the speed of the stage. These results show that this type of fabrication setup is suitable for fabrication of patterns of biological molecules.
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