Two-photon polymerization (TPP, 2PP) has been utilized in fluorescence microscopy but it is also a powerful 3D microfabrication technique for manufacturing of submicron structures and patterns of biological molecules. TPP provides an efficient way to pattern surfaces of cell culture dishes or tissue engineering scaffolds and to guide the growth of cultivated cells. In this study the TPP process was utilized to crosslink two biologically relevant proteins – avidin and bovine serum albumin. The polymerization setup included a frequency doubled, diode-pumped Q-switched Nd:YAG pulsed laser, which was operated at 532 nm. Submicron protein strings were fabricated by moving a motorized X-Y-Z stage at a suitable rate along a predefined path. The width of the protein patterns could be varied by changing the laser power and the speed of the stage. These results show that this type of fabrication setup is suitable for fabrication of patterns of biological molecules.
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ICALEO 2008: 27th International Congress on Laser Materials Processing, Laser Microprocessing and Nanomanufacturing
October 20–23, 2008
Temecula, California, USA
ISBN:
978-0-912035-12-3
PROCEEDINGS PAPER
Two-photon polymerization of protein patterns with Nd:YAG pulsed laser Available to Purchase
Sanna Peltola;
Sanna Peltola
1
Department of Biomedical Engineering, Tampere University of Technology
, P.O. Box 692, 33101, Tampere, Finland
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Minna Kellomäki;
Minna Kellomäki
1
Department of Biomedical Engineering, Tampere University of Technology
, P.O. Box 692, 33101, Tampere, Finland
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Jouko Viitanen
Jouko Viitanen
2
VTT Technical Research Centre of Finland
, P.O. Box 1300, 33101 Tampere, Finland
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Published Online:
October 01 2008
Citation
Sanna Peltola, Minna Kellomäki, Jouko Viitanen; October 20–23, 2008. "Two-photon polymerization of protein patterns with Nd:YAG pulsed laser." Proceedings of the ICALEO 2008: 27th International Congress on Laser Materials Processing, Laser Microprocessing and Nanomanufacturing. ICALEO 2008: 27th International Congress on Laser Materials Processing, Laser Microprocessing and Nanomanufacturing. Temecula, California, USA. (pp. N403). ASME. https://doi.org/10.2351/1.5061420
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