... excitation and emission. In the next subsection, we consider both cases for widefield fluorescence microscopy. Widefield Fluorescence Microscopy Freely rotating dipole molecules Consider a situation where the illumination is randomly or circularly polarized and the fluorescent molecule can freely rotate...

... the rules: Widefield microscopy and the Abbe limit of resolution: High resolution widefield microscopy ,” J. Cell. Physiol.   229 ( 2 ), 132 – 138 ( 2014 ). 10.1002/jcp.24439 Vicidomini , G. , Bianchini , P. , and Diaspro , A. , “ STED super-resolved microscopy ,” Nat. Methods   15...

.../ncomms4936 Chakraborty , S. , Nian , F.-S. , Tsai , J.-W. , Karmenyan , A. , and Chiou , A. , “ Quantification of the metabolic state in cell-model of Parkinson's disease by fluorescence lifetime imaging microscopy ,” Sci. Rep.   6 , 19145 ( 2016 ). 10.1038/srep19145...

.../ncomms4936 Chakraborty , S. , Nian , F.-S. , Tsai , J.-W. , Karmenyan , A. , and Chiou , A. , “ Quantification of the metabolic state in cell-model of Parkinson's disease by fluorescence lifetime imaging microscopy ,” Sci. Rep.   6 , 19145 ( 2016 ). 10.1038/srep19145...

...One-photon fluorescence microscopy widefield microscopy light-field microscopy confocal microscopy light-sheet microscopy neuroscience Introduction The development of the one-photon fluorescence microscope traces from the turn of the first millennium past the turn of the second millennium...

... the rules: Widefield microscopy and the Abbe limit of resolution: High resolution widefield microscopy ,” J. Cell. Physiol.   229 ( 2 ), 132 – 138 ( 2014 ). 10.1002/jcp.24439 Vicidomini , G. , Bianchini , P. , and Diaspro , A. , “ STED super-resolved microscopy ,” Nat. Methods   15...

.... , “ Whole-brain functional imaging at cellular resolution using light-sheet microscopy ,” Nat. Methods   10 ( 5 ), 413 – 420 ( 2013 ). 10.1038/nmeth.2434 Andermann , M. L. , Gilfoy , N. B. , Goldey , G. J. , Sachdev , R. N. , Wölfel , M. , McCormick , D. A.   , “ Chronic...

...Microscopy optical slit Nyquist criterion diffraction Introduction The generation of a planar field is crucial for real-time single-shot sectional imaging of large specimens such as Drosophila larvae and zebrafish embryos. Traditional microscopes have been quite successful for investigating...

... , S.   , “ High-speed widefield photoacoustic microscopy of small-animal hemodynamics ,” Biomed. Opt. Express   9 ( 10 ), 4689 – 4701 ( 2018 ). 10.1364/BOE.9.004689 Lee , J. , Han , S. , Seong , D. , Lee , J. , Park , S. , Wijesinghe , R. E.   , “ Fully waterproof...

... include widefield, confocal, two-photon microscopy, and their variants ( Minsky, 1961 ; Minsky, 1988 ; Denk et al., 1990 ; Pawley, 2006 ; Diaspro et al., 2017 ; and Diaspro, 2018 ). In widefield microscopy, light is focused by a high-numerical-aperture (high-NA) objective lens...

...Microscopy Acoustic microscopy Polarization Transverse effects Maxwell equations Electromagnetism Introduction The next-generation biomedical technologies demand rapid interrogation of biological specimens (cells and organisms) and determination of critical biophysical parameters. Existing...

...Microscopy Acoustic microscopy Polarization Transverse effects Electromagnetism Bessel field optical tweezers Introduction In the previous chapter, we have briefly compared the light sheet technique with the point-illumination technique. It is immediately evident that light sheet has better...

...Front Matter Light Sheet Microscopy and Imaging introduces the fundamentals and important advancements at the frontiers of optical physics and applied sciences. It presents coherent, in-depth explanations of different variants of light sheet microscopes beginning from the basics of light...

.... , Liu , W. , Wang , Y. C. , Shi , J. H. , Li , Y. , Xu , S.   , “ High-speed widefield photoacoustic microscopy of small-animal hemodynamics ,” Biomed. Opt. Express   9 ( 10 ), 4689 – 4701 ( 2018 ). 10.1364/BOE.9.004689 Lee , J. , Han , S. , Seong , D. , Lee...

... . Batista , A. , Breunig , H. G. , Donitzky , C. , and König , K. , “ 16 Two-photon microscopy and fluorescence lifetime imaging of the cornea ,” in Multiphoton Microscopy and Fluorescence Lifetime Imaging ( De Gruyter , 2018 ), pp. 301 – 320 . Bélanger , S. , Abran , M...

...Microscopy Acoustic microscopy Polarization Transverse effects Maxwell equations Electromagnetism Existing Point-Illumination-Based Technology Existing technologies are heavily dependent on point illumination across the spectrum of sciences and technology. Although this is reaping benefits...