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Book Chapter
Series: AIPP Books, Methods
Published: December 2021
10.1063/9780735423664_004
EISBN: 978-0-7354-2366-4
ISBN: 978-0-7354-2364-0
...Hepburn, M. S., Foo, K. Y., Curatolo, A., Munro, P. R. T., and Kennedy, B. F., “Speckle in optical coherence tomography, ” in Optical Coherence Elastography: Imaging Tissue Mechanics on the Micro-Scale, edited by B. F. Kennedy (AIP Publishing, Melville, New York, 2021), pp. 4-1–4-30...
Images
Illustrating bright and dark speckle in one dimension. (a) An array of scatterers and (b) the corresponding OCT signal exhibiting regions of bright and dark speckle.
Published: December 2021
FIG. 4.3 Illustrating bright and dark speckle in one dimension. (a) An array of scatterers and (b) the corresponding OCT signal exhibiting regions of bright and dark speckle. More about this image found in Illustrating bright and dark speckle in one dimension. (a) An array of scat...
Images
(a) Speckle contrast as a function of the number of averaged independent speckle realizations, N, for amplitude and intensity. (b) Speckle SNR as a function of N for amplitude and intensity.
Published: December 2021
FIG. 4.10 (a) Speckle contrast as a function of the number of averaged independent speckle realizations, N, for amplitude and intensity. (b) Speckle SNR as a function of N for amplitude and intensity. More about this image found in (a) Speckle contrast as a function of the number of averaged independent sp...
Images
(a) Speckle contrast and (b) SNR in a region containing both a specular reflection and diffuse scatterers (resulting in a constant phasor plus random phasor sum) as a function of r.
Published: December 2021
FIG. 4.13 (a) Speckle contrast and (b) SNR in a region containing both a specular reflection and diffuse scatterers (resulting in a constant phasor plus random phasor sum) as a function of r. More about this image found in (a) Speckle contrast and (b) SNR in a region containing both a specular ref...
Images
Illustration of the relationship between speckle size and OCT resolution. (a) Simulated intensity for a single scatterer (representing the 2D PSF). (b) Simulated speckle pattern produced by a distribution of scatterers. (c) Plots comparing the PSF (blue) along the x-axis with the theoretical speckle correlation function in Eq. (4.45) (red), as well as the average cross-correlation generated using (b). Scale bars represent 20 µm.
Published: December 2021
FIG. 4.14 Illustration of the relationship between speckle size and OCT resolution. (a) Simulated intensity for a single scatterer (representing the 2D PSF). (b) Simulated speckle pattern produced by a distribution of scatterers. (c) Plots comparing the PSF (blue) along the x-axis More about this image found in Illustration of the relationship between speckle size and OCT resolution. (...
Images
Demonstration of the relationship between speckle size and OCT resolution. Both images are of the same mouse aorta; however, (a) was taken on a system with an axial and lateral resolution (in air) of 7.8 µm and 11 µm, respectively, while (b) was taken on a system with a higher axial and lateral resolution of 1.5 µm and 1.6 µm, respectively. Example regions of elastin sheets (bright wavy lines) and smooth muscle (dark wavy lines) are indicated by the red and blue arrows, respectively. Note the increase in the visibility of structures that have dimensions well above the OCT resolution in the second case.
Published: December 2021
FIG. 4.15 Demonstration of the relationship between speckle size and OCT resolution. Both images are of the same mouse aorta; however, (a) was taken on a system with an axial and lateral resolution (in air) of 7.8 µm and 11 µm, respectively, while (b) was taken on a system with a higher axial More about this image found in Demonstration of the relationship between speckle size and OCT resolution. ...
Images
Reducing speckle contrast using frequency compounding. OCT intensity B-scans of the same scattering phantom acquired with (a) a light source with a central wavelength of 1312 nm and a FWHM bandwidth of 36 nm and (b) a central wavelength of 1488 nm and a FWHM bandwidth of 57 nm. The measured speckle contrast was ∼0.63 in (a) and ∼0.64 in (b). Through averaging, the measured speckle contrast in (c) is improved by a factor of 1.25.
Published: December 2021
FIG. 4.16 Reducing speckle contrast using frequency compounding. OCT intensity B-scans of the same scattering phantom acquired with (a) a light source with a central wavelength of 1312 nm and a FWHM bandwidth of 36 nm and (b) a central wavelength of 1488 nm and a FWHM bandwidth of 57 nm More about this image found in Reducing speckle contrast using frequency compounding. OCT intensity B-scan...
Images
(a) OCT and (b) speckle-modulating (SM) OCT B-scans of a mouse cornea. (c) and (d) are magnified views of the regions indicated by yellow boxes in (a) and (b), respectively. (e) Microscope image of hematoxylin and eosin (H&E) stained mouse cornea at ten times magnification. Scale bar: 100 µm. Adapted from Liba, O. et al., Nat. Commun. 8(1), 15845 (2017). Copyright 2017 Springer Nature.
Published: December 2021
FIG. 4.17 (a) OCT and (b) speckle-modulating (SM) OCT B-scans of a mouse cornea. (c) and (d) are magnified views of the regions indicated by yellow boxes in (a) and (b), respectively. (e) Microscope image of hematoxylin and eosin (H&E) stained mouse cornea at ten times magnification. Scale More about this image found in (a) OCT and (b) speckle-modulating (SM) OCT B-scans of a mouse cornea. (c) ...
Images
The impact of speckle on the accuracy of phase-sensitive OCT. Reference and translated scatterer locations, and corresponding OCT intensity, of (a) a single scatterer (specular reflector), (b) five scatterers constructively interfering (bright speckle), and (c) five scatterers destructively interfering (dark speckle) undergoing a π radian phase shift. Phasor representations of the reference and translated OCT signals evaluated at z = 0 for (d) a single scatterer, (e) five scatterers in bright speckle, and (f) five scatterers in dark speckle.
Published: December 2021
FIG. 5.5 The impact of speckle on the accuracy of phase-sensitive OCT. Reference and translated scatterer locations, and corresponding OCT intensity, of (a) a single scatterer (specular reflector), (b) five scatterers constructively interfering (bright speckle), and (c) five scatterers More about this image found in The impact of speckle on the accuracy of phase-sensitive OCT. Reference and...
Images
Images
Changes of a speckle pattern in a homogeneous silicone phantom under an increasing compressive load applied to the top of the sample from (a) to (d). Note that the shape of the black outline changes as the load increases, highlighting changes in the speckle pattern. Image dimensions are 50 × 50 µm2.
Published: December 2021
FIG. 5.14 Changes of a speckle pattern in a homogeneous silicone phantom under an increasing compressive load applied to the top of the sample from (a) to (d). Note that the shape of the black outline changes as the load increases, highlighting changes in the speckle pattern. Image dimensions More about this image found in Changes of a speckle pattern in a homogeneous silicone phantom under an inc...
Images
Simulated OCT amplitude B-scans generated by averaging (a) 1, (b) 10, and (c) 100, independent speckle realizations. (d), (e), and (f) are speckle intensity B-scans from the speckle amplitudes in (a), (b), and (c), respectively. The speckle contrast in the OCT amplitude B-scans in (a), (b), and (c) is ∼0.52, ∼0.16, and ∼0.05, respectively. The speckle contrast in the OCT intensity B-scans in (d), (e), and (f) is ∼1.0, ∼0.32, and ∼0.10, respectively. Scale bars represent 20 µm.
Published: December 2021
FIG. 4.9 Simulated OCT amplitude B-scans generated by averaging (a) 1, (b) 10, and (c) 100, independent speckle realizations. (d), (e), and (f) are speckle intensity B-scans from the speckle amplitudes in (a), (b), and (c), respectively. The speckle contrast in the OCT amplitude B-scans More about this image found in Simulated OCT amplitude B-scans generated by averaging (a) 1, (b) 10, and (...
Images
Scatterer distributions with different densities in (a), (b), and (c). Illustration of (d) no speckle, (e) partially developed speckle, and (f) fully developed speckle in the OCT amplitude corresponding to the scatterer distributions in (a), (b), and (c), respectively. Scale bars represent 20 µm.
Published: December 2021
FIG. 4.6 Scatterer distributions with different densities in (a), (b), and (c). Illustration of (d) no speckle, (e) partially developed speckle, and (f) fully developed speckle in the OCT amplitude corresponding to the scatterer distributions in (a), (b), and (c), respectively. Scale bars More about this image found in Scatterer distributions with different densities in (a), (b), and (c). Illu...
Book Chapter
Book cover for Ultrafast Imaging and Spectroscopy for Biomedicine
Series: AIPP Books, Principles
Published: May 2022
10.1063/9780735424678_009
EISBN: 978-0-7354-2467-8
ISBN: 978-0-7354-2464-7
... to that of medical ultrasound. Its high axial resolution is good at diagnosing layered structures like retina ( Swanson et al., 1993 ), blood vessels ( Jang et al., 2002 ), or esophagus ( Evans et al., 2006 ). Combined with optical doppler or speckle imaging, OCT can capture information...
Images
B-scans of (a) amplitude, (b) intensity, and (c) phase for fully developed speckle. Analytical PDFs of (d) amplitude, (e) intensity, and (f) phase in fully developed speckle. Scale bars represent 20 µm.
Published: December 2021
FIG. 4.8 B-scans of (a) amplitude, (b) intensity, and (c) phase for fully developed speckle. Analytical PDFs of (d) amplitude, (e) intensity, and (f) phase in fully developed speckle. Scale bars represent 20 µm. More about this image found in B-scans of (a) amplitude, (b) intensity, and (c) phase for fully developed ...
Images
OCT image of a human fingertip plotted on a logarithmic scale. A sweat duct obscured by speckle is highlighted in the inset. Examples of bright and dark speckle are indicated by the blue and red arrows, respectively. Scale bars represent 100 µm.
Published: December 2021
FIG. 4.1 OCT image of a human fingertip plotted on a logarithmic scale. A sweat duct obscured by speckle is highlighted in the inset. Examples of bright and dark speckle are indicated by the blue and red arrows, respectively. Scale bars represent 100 µm. More about this image found in OCT image of a human fingertip plotted on a logarithmic scale. A sweat duct...
Book Chapter
Book cover for Ultrafast Imaging and Spectroscopy for Biomedicine
Series: AIPP Books, Principles
Published: May 2022
10.1063/9780735424678_007
EISBN: 978-0-7354-2467-8
ISBN: 978-0-7354-2464-7
... speckle information can serve as a measure to evaluate the short-term and short-range coherence. The autocorrelation measurement of these fluctuated scattering signals could provide a sense of coherence length. Fast diffusion will decrease the amplitude of autocorrelation quickly with time delay...
Book
Book cover for Ultrafast Imaging and Spectroscopy for Biomedicine
Series: AIPP Books, Principles
Published: May 2022
10.1063/9780735424678
EISBN: 978-0-7354-2467-8
ISBN: 978-0-7354-2464-7
Images
OCT, SNR, axial displacement, and axial strain A-scans of a mechanically homogeneous sample using compression OCE; (a)–(c) are generated without speckle reduction, and (d)–(f) are generated with speckle reduction brought about by spatial averaging.
Published: December 2021
FIG. 4.19 OCT, SNR, axial displacement, and axial strain A-scans of a mechanically homogeneous sample using compression OCE; (a)–(c) are generated without speckle reduction, and (d)–(f) are generated with speckle reduction brought about by spatial averaging. Adapted from Hepburn, M. S. et More about this image found in OCT, SNR, axial displacement, and axial strain A-scans of a mechanically ho...
Book Chapter
Series: AIPP Books, Methods
Published: December 2021
0
EISBN: 978-0-7354-2366-4
ISBN: 978-0-7354-2364-0
...References References Adler , D. C. , Ko , T. H. , and Fujimoto , J. G. , “ Speckle reduction in optical coherence tomography images by use of a spatially adaptive wavelet filter ,” Opt. Lett.   29 ( 24 ), 2878 – 2880 ( 2004 ). 10.1364/OL.29.002878 Bashkansky , M...