X-ray photoelectron spectroscopy was used to analyze commercially available powders of thymine, uracil, cytosine, guanine, adenine, as well as DNA isolated from salmon testes and RNA from torula yeast. The powders were pressed onto a sample holder and analyzed as received from the manufacturer. Survey spectra and high-resolution spectra of O 1s, N 1s, C 1s of all nucleobases are reported, including a small Na 1s contamination in cytosine. Spectra of DNA and RNA are also included for comparison. Furthermore, the presented data are compared with previously published results, as well as theoretical values, and differences are discussed.

  • Accession#: 01822, 01823, 01824, 01825, 01826, 01827, 01828

  • Technique: XPS

  • Host Material: Nucleobases, DNA, and RNA

  • Instrument: Kratos Axis Ultra DLD

  • Major Elements in Spectra: O, N, C

  • Minor Elements in Spectra: Na

  • Published Spectra: 32

  • Spectral Category: Comparison

Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) consist of a backbone of sugar units linked with phosphate moieties. Each sugar unit links to a nucleobase and the order of bases build up the genetic code being stored or transcribed (Fig. 1). These macromolecular substances have varying elemental atomic ratios depending on the sequence of the individual bases in the sample. This compositional information will be averaged and overlaid in XPS spectra from the macromolecular substances.

FIG. 1.

Five nucleobases (a) adenine, (b) guanine, (c) cytosine, (d) thymine, and (e) uracil. The numbers represent assignment of C components in C 1s spectra from low BE (1 for CH) to high (5). (f) A generalized structure showing a nucleotide repeat unit building up RNA (here, adenosine). Each base links to a backbone of sugar and phosphate. Individual nucleotides are linked via 3′, 5′-phosphodiester bonds. DNA differs from RNA by one OH group on the 2′ position of the sugar unit carrying the base being replaced by H in DNA. In addition, RNA contains the base uracil instead of thymine, both forming base pairs with adenine.

FIG. 1.

Five nucleobases (a) adenine, (b) guanine, (c) cytosine, (d) thymine, and (e) uracil. The numbers represent assignment of C components in C 1s spectra from low BE (1 for CH) to high (5). (f) A generalized structure showing a nucleotide repeat unit building up RNA (here, adenosine). Each base links to a backbone of sugar and phosphate. Individual nucleotides are linked via 3′, 5′-phosphodiester bonds. DNA differs from RNA by one OH group on the 2′ position of the sugar unit carrying the base being replaced by H in DNA. In addition, RNA contains the base uracil instead of thymine, both forming base pairs with adenine.

Close modal

This data set has previously been published by us as part of supporting information to a publication describing surface characterization of fungi and viruses (Ref. 1). However, they were not discussed individually or individually assigned. The XPS data from microorganisms, in that publication, indicated no presence of nucleic acids at the sample surface at the information depth of XPS.

From the chemical structure of the bases it can be concluded that several types of chemical shifts are to be expected and some these are not commonly seen for other biological building blocks. For example, the binding energy for carbon in the sub structures: HN–(C=O)–NH in uracil, thymine, and cytosine, as well as the C in N–(C–NH2)–NH in guanine are rare in other building blocks for biological macromolecules but are present in nucleic acids and nucleotides.

Computational studies, based on density functional theory calculations as well as ab initio calculations, led Sun et al. to suggest that the two bases guanine and adenine have some degree of aromatic property but the other three bases do not (Ref. 2). Thus, in this work, we have assumed a position of 285.0 eV for C atoms without polar neighbors (CH) in uracil, thymine, and cytosine. The binding energy scale in spectra from guanine and adenine, lacking CH, were calibrated to give a binding energy of 286.0 eV for CN in C 1s spectra. In C 1s spectra of all the bases, we have assigned: CN at 286.0–286.7 eV, NCN at 287.1–287.7, N–(C=O), N–(CNH2)–N, and N–(C=O)–N= at 288.2–288.8 eV, and finally, N–(C=O)–N at 289.4–289.6 eV. We have, in this work, not considered secondary shifts in BE.

Several resonance structures are predicted for the bases (Ref. 2) that could give rise to broadening of peaks and relative changes in peak intensities. This may explain the small asymmetry observed on the high BE side in some peaks and differences in observed atomic ratios when theoretical values based on the structures in Fig. 1 and experimental values are compared (Tables I and II). An example of such differences is seen in the ratios between N-atoms in the N peak for guanine and adenine.

Table I.

Comparison between theoretical (based on Fig. 1) and experimental elemental atomic % for nucleobases.

CON
Theoretical 
Uracil 50.0 25.0 25.0 
Thymine 55.6 22.2 22.2 
Cytosine 50.0 12.5 37.5 
Guanine 45.5 9.1 45.5 
Adenine 50.0 0.0 50.0 
Experimental 
Uracil 54.0 21.9 24.1 
Thymine 58.0 19.6 22.4 
Cytosine 51.6 11.3 36.8 
Guanine 45.2 8.2 46.6 
Adenine 50.2 0.8 49.0 
CON
Theoretical 
Uracil 50.0 25.0 25.0 
Thymine 55.6 22.2 22.2 
Cytosine 50.0 12.5 37.5 
Guanine 45.5 9.1 45.5 
Adenine 50.0 0.0 50.0 
Experimental 
Uracil 54.0 21.9 24.1 
Thymine 58.0 19.6 22.4 
Cytosine 51.6 11.3 36.8 
Guanine 45.2 8.2 46.6 
Adenine 50.2 0.8 49.0 
Table II.

Comparison between theoretical (based on Fig. 1) and experimental ratios between C components and total C for nucleobases.

C1C2C3C4C5
Theoretical 
Uracil 25 25 — 25 25 
Thymine 40 20 — 20 20 
Cytosine 25 25 25 25 — 
Guanine — 20 40 40 — 
Adenine — 20 80 — — 
Experimental 
Uracil 31.5 25.5 — 18.9 24.3 
Thymine 42.5 20.5 — 17.3 19.6 
Cytosine 27.1 23.4 21.6 27.9 — 
Guanine — 24.8 38.7 36.4 — 
Adenine — 20.9 79.1 — — 
C1C2C3C4C5
Theoretical 
Uracil 25 25 — 25 25 
Thymine 40 20 — 20 20 
Cytosine 25 25 25 25 — 
Guanine — 20 40 40 — 
Adenine — 20 80 — — 
Experimental 
Uracil 31.5 25.5 — 18.9 24.3 
Thymine 42.5 20.5 — 17.3 19.6 
Cytosine 27.1 23.4 21.6 27.9 — 
Guanine — 24.8 38.7 36.4 — 
Adenine — 20.9 79.1 — — 

In cytosine a small quantity of Na+ was detected. This most likely is a minor contamination. All bases are neutrally charged at physiologic pH (Ref. 3). Adenine, cytosine and guanine are reported to become protonated below pH 4 and amine groups in guanine thymine and uracil are deprotonated above pH 9 (Ref. 3). Furthermore, a small quantity of O was detected in adenine and is also assumed to be a contamination.

Previous work has described that DNA degrades under the x-ray beam starting with alterations in N after ca 1 h exposure (Mg Kα 260 W at 13 kV) with 15% loss of imino N at 400 eV (Ref. 4). Thus, intensity variations in O and N could also be due to alterations relating to sample exposure to x rays. However, the high- resolution data for O, N and C from nucleobases, presented here, were all acquired using monochromatic Al Kα x rays and analyses times between 1488 and 1856 s, i.e., ca. 25–30 min (using 150 W).

The relative contribution of elements at the surface has previously been analyzed with XPS and reported for nucleobases. These values are in close agreement with what was observed here (Table III) (Refs. 4 and 5). Binding energy values for N 1s and O 1s are in good agreement between this dataset and previously published data (Table IV) (Ref. 6). However, some differences exist in the assigned binding energy positions for the fitted C components (Table V) (Ref. 6) and in the number of peaks used to fit the early literature data and the data presented here, for the bases thymine and guanine. This may be due to differences in peak resolution between instruments as well as fitting approach between the study from 1978 and this one.

Table III.

Literature values for elemental composition in atomic % for nucleobases compared to this study.

ReferenceNucleobaseCON
Theory — 50.0 — 50.0 
Ptasinska 2008 Adenine 49.6 — 50.5 
May 2004 — 52.5 — 47.5 
This work — 50.2 0.8 49.0 
Theory — 50.0 12.5 37.5 
Ptasinska 2008 Cytosine 49.7 13.2 37.2 
May 2004 — 54.5 11.3 34.1 
This work — 51.6 11.3 36.8 
Theory — 45.5 9.1 45.5 
Ptasinska 2008 Guanine 44.4 9.8 45.9 
May 2004 — 46.9 9.1 44.0 
This work — 45.2 8.2 46.6 
Theory — 55.6 22.2 22.2 
Ptasinska 2008 Thymine 55.6 22.7 21.8 
May 2004 — 59.7 19.2 21.1 
This work — 58.0 19.6 22.4 
Theory — 50.0 25.0 25.0 
Ptasinska 2008 Uracil — — — 
May 2004 — 52.2 23.0 24.7 
This work — 54.0 21.9 24.1 
ReferenceNucleobaseCON
Theory — 50.0 — 50.0 
Ptasinska 2008 Adenine 49.6 — 50.5 
May 2004 — 52.5 — 47.5 
This work — 50.2 0.8 49.0 
Theory — 50.0 12.5 37.5 
Ptasinska 2008 Cytosine 49.7 13.2 37.2 
May 2004 — 54.5 11.3 34.1 
This work — 51.6 11.3 36.8 
Theory — 45.5 9.1 45.5 
Ptasinska 2008 Guanine 44.4 9.8 45.9 
May 2004 — 46.9 9.1 44.0 
This work — 45.2 8.2 46.6 
Theory — 55.6 22.2 22.2 
Ptasinska 2008 Thymine 55.6 22.7 21.8 
May 2004 — 59.7 19.2 21.1 
This work — 58.0 19.6 22.4 
Theory — 50.0 25.0 25.0 
Ptasinska 2008 Uracil — — — 
May 2004 — 52.2 23.0 24.7 
This work — 54.0 21.9 24.1 
Table IV.

Comparison of N and O binding energies (eV) reported previously and in this study for three of the nucleobases.

Pelling 1978UracilThymineGuanine
400.8 400.9 399.5 
— — 400.8 
531.8 532 531.8 
This study Uracil Thymine Guanine 
400.8 400.6 399.6 
— — 401.0 
531.9 531.7 532.1 
Pelling 1978UracilThymineGuanine
400.8 400.9 399.5 
— — 400.8 
531.8 532 531.8 
This study Uracil Thymine Guanine 
400.8 400.6 399.6 
— — 401.0 
531.9 531.7 532.1 
Table V.

Comparison of C1s literature values for binding energy positions (eV) and chemical shifts for three nucleobases compared to this study.

Pelling 1978a Uracil Thymine Guanine 
C1 285.6 285.4 285.8 
C2 286.8 285.8 286.5 
C3 — 286.6 287.2 
C4 288.9 288.7 288.5 
C5 289.7 289.8 288.7 
Chemical shift — — — 
Δ1 0.6 0.4 0.8 
Δ2 1.8 0.8 1.5 
Δ3  1.6 2.2 
Δ4 3.9 3.7 3.5 
Δ5 4.7 4.8 3.7 
This study Uracil Thymine Guanine 
C1 285.0 285.0 — 
C2 286.7 286.2 286.0 
C3 — — 287.1 
C4 288.7 288.3 288.8 
C5 289.6 289.4 — 
Chemical shift — — — 
Δ1 0.0 0.0 — 
Δ2 1.7 1.2 1.0 
Δ3 — — 2.1 
Δ4 3.7 3.3 3.8 
Δ5 4.6 4.4 — 
Pelling 1978a Uracil Thymine Guanine 
C1 285.6 285.4 285.8 
C2 286.8 285.8 286.5 
C3 — 286.6 287.2 
C4 288.9 288.7 288.5 
C5 289.7 289.8 288.7 
Chemical shift — — — 
Δ1 0.6 0.4 0.8 
Δ2 1.8 0.8 1.5 
Δ3  1.6 2.2 
Δ4 3.9 3.7 3.5 
Δ5 4.7 4.8 3.7 
This study Uracil Thymine Guanine 
C1 285.0 285.0 — 
C2 286.7 286.2 286.0 
C3 — — 287.1 
C4 288.7 288.3 288.8 
C5 289.6 289.4 — 
Chemical shift — — — 
Δ1 0.0 0.0 — 
Δ2 1.7 1.2 1.0 
Δ3 — — 2.1 
Δ4 3.7 3.3 3.8 
Δ5 4.6 4.4 — 
a

Peak width FWHM 1.4–1.6 eV, CH referenced to unique C contamination at 285.0 eV.

From the presented dataset it is clear that most of the fine structure detail observed in C 1s spectra of the individual nucleobases are difficult to resolve in samples prepared from the commercially available DNA and RNA powders. Instead, the fitting of C 1s spectra of the macromolecules give rise to components with larger FWHM than what was observed for the individual samples of nucleobases, indicating several species overlapping in the spectra as well as intermolecular bonding between nucleobases within the macromolecular structure. However, the elemental atomic percentage corresponded surprisingly well between a theoretical estimate and the experimental data (Table VI), especially if a contribution of some level of adventitious C is assumed. The theoretical content used here for the nucleic acids was calculated assuming that the nucleotides were present at equal amounts in both the two commercial powders of DNA and RNA.

Table VI.

Comparison between average (av.) theoretical and experimental atomic % in DNA and RNA assuming equal contributions of each nucleotide.

CNOP
Adenosine 48 24 24 
Cytidine 47 16 32 
Guanosine 45 23 27 
Thymidine 50 10 35 
Av. DNA 48 18 29 
Adenosine 45 23 27 
Cytidine 45 15 35 
Guanosine 43 22 30 
Uracil 45 10 40 
Av. RNA 45 17 33 
Experimental 
DNA 52.1 15.1 25.0 3.6 
RNA 49.7 14.5 31.9 3.9 
Experimental (Tougaard) 
DNA 52.5 14.9 24.9 3.5 
RNA 50.2 14.5 31.5 3.9 
CNOP
Adenosine 48 24 24 
Cytidine 47 16 32 
Guanosine 45 23 27 
Thymidine 50 10 35 
Av. DNA 48 18 29 
Adenosine 45 23 27 
Cytidine 45 15 35 
Guanosine 43 22 30 
Uracil 45 10 40 
Av. RNA 45 17 33 
Experimental 
DNA 52.1 15.1 25.0 3.6 
RNA 49.7 14.5 31.9 3.9 
Experimental (Tougaard) 
DNA 52.5 14.9 24.9 3.5 
RNA 50.2 14.5 31.5 3.9 

Host Material: Uracil (01822), thymine (01823), cytosine (01824), guanine (01825), adenine (01826), DNA from salmon testes (01827), and RNA from torula yeast (01828)

CAS Registry #: 66-22-8; 65-71-4; 71-30-7; 73-40-5; 73-24-5; 9007-49-2; 63231-63-0

Host Material Characteristics: Homogeneous, solid, unknown crystallinity, dielectric, biological material, others

Chemical Name: N/A

Source: Sigma-Aldrich/Merck provided DNA, RNA, uracil, guanin, cytosine. Adenine came from Fluka and thymine from Koch

Host Composition: The commercial powers were analyzed as received

Form: Powder

Structure: N/A

History and Significance: These references are important for assignment of spectra from biological samples containing nucleobases, nucleotides, or DNA/RNA at the surface.

As Received Condition: The commercially available reference samples were received as powders

Analyzed Region: The same as host material

Ex Situ Preparation/Mounting: The powder was gently ground in a mortar and thereafter placed onto the sample holder using a spatula. Thereafter, the powder was gently pressed to form a smooth surface.

In Situ Preparation: Analysis was done at room temperature, 293 K.

Charge Control: Charge neutralizing using the built-in charge neutralizing system of the instrument. Filament current 1.8 A, Charge balance 3.2 V, Filament bias 1.2 V, Magnetic lens trim coil 0.2 A

Temp. During Analysis: 293 K

Pressure During Analysis: <1.3 × 10−7 Pa

Pre-analysis Beam Exposure: ca. 300 s

Manufacturer and Model: Kratos Axis Ultra DLD

Analyzer Type: Spherical sector

Detector: Multichannel resistive plate

Number of Detector Elements: 128

Analyzer Mode: Constant pass energy

Throughput (T = EN): N = 0

Excitation Source Window: Not specified

Excitation Source: Al Kα monochromatic

Source Energy: 1486.7 eV

Source Strength: 150 W, User defined

Source Beam Size: 2000 × 1000 μm2

Signal Mode: Multichannel direct

Incident Angle: 54°

Source-to-Analyzer Angle: 54°

Emission Angle:

Specimen Azimuthal Angle: 45°

Acceptance Angle from Analyzer Axis:

Analyzer Angular Acceptance Width: 40° × 40°

Energy Scale Correction: Calibrated to aliphatic C component in C 1s at 285.0 eV following ISO standards 19318:2004 and 15472:2010. For nucleobases lacking CH the BE scale was instead referenced to the CN component at 286.0 eV in C 1s spectra. Our spectrometer is calibrated once per year according to ISO standard 15472.

Recommended Energy Scale Shift: Sample dependent, please consult the Guide to Figures Table below.

Peak Shape and Background Method: GL30, Shirley background. Spectra were fitted using Casa XPS with as few components as possible and without further restrictions except for P 2p, where 2p1/2 and 2p3/2 were linked, or when specifically indicated in the spectral features table. FWHM were not fixed, if the range was restricted it is specified in spectral features table. In general, restrictions were only made in C 1s to prevent peak shapes to become narrower than 1 eV. In our experience, more narrow peak widths are mainly observed in conjugated systems such as graphite, with our type of instrumentation, and then also gives rise to asymmetry in the peak. In addition to the above peak fitting, the entire dataset was also fitted using Tougaard background instead of Shirley (data not shown). No significant differences were observed in the resulting data between the two backgrounds. A selection of these results is shown in Tables VI.

Quantitation Method: Based on peak areas and relative sensitivity factors (Ref. 7). Quantification was based on the area in high-resolution spectra. The small contributions from π–π excitations were not fitted.

SPECTRAL FEATURES TABLEa

Spectrum ID #Element/transitionPeak energy (eV)Peak width FWHM (eV)Peak area (eV × cts/s)Sensitivity factorConcentration (at. %)Peak assignment
01822-01 Survey — — — — — — 
01822-02 O 1s 531.9 1.3 23366 0.78 21.9 Double bonded O 
01822-03 N 1s 400.8 1.2 15687 0.477 24.1 HN–C=O 
01822-04b C 1s 289.6 1.0 4990 0.278 13.1 –NH–(C=O)–NH– 
01822-04b C 1s 288.7 1.0 3880 0.278 10.2 –NH–(C=O)– 
01822-04b C 1s 286.7 1.1 5178 0.278 13.6 CN 
01822-04b C 1s 285.0 1.0 6472 0.278 17.0 CH 
01823-01 Survey … … … … … … 
01823-02 O 1s 531.7 1.3 19630 0.78 19.6 Double bonded O 
01823-03 N 1s 400.6 1.1 13732 0.477 22.4 HN–C=O 
01823-04b C 1s 289.4 1.0 4068 0.278 11.4 –NH–(C=O)–NH– 
01823-04b C 1s 288.3 1.0 3592 0.278 10.1 –NH–(C=O)– 
01823-04b C 1s 286.2 1.0 4259 0.278 11.9 CN 
01823-04b C 1s 285.0 1.0 8818 0.278 24.7 CH (x2) 
01824-01 Survey — — — — — — 
01824-02 Na 1s 1071.9 1.6 634 1.685 0.3 Na+ 
01824-03 O 1s 531.4 1.3 11044 0.78 11.3 double bonded O 
01824-04f N 1s 400.1 1.4 14657 0.477 24.5 –NH– 
01824-04f N 1s 399.0 1.2 7331 0.477 12.3 –N= 
01824-05c C 1s 288.8 1.1 5011 0.278 14.4 –NH–(C=O)–N= 
01824-05c C 1s 287.7 1.0 3891 0.278 11.2 –N–C–N– 
01824-05c C 1s 286.5 1.0 4207 0.278 12.1 CN 
01824-05c C 1s 285.0 1.1 4874 0.278 14.0 CH 
01825-01 Survey — — — — — — 
01825-02 O 1s 532.1 1.2 9024 0.78 8.2 Double bonded O 
01825-03 N 1s 401.0 1.4 15749 0.477 23.4 NH/NH2 
01825-03 N 1s 399.6 1.1 15614 0.477 23.2 –N=/NH 
01825-04 C 1s 288.8 1.0 6461 0.278 16.5 –NH–(C–NH2)–NH– & –NH–(C=O)– 
01825-04 C 1s 287.1 1.0 6866 0.278 17.5 –N–C–N– 
01825-04 C 1s 286.0 1.1 4404 0.278 11.2 CN 
01826-01 Survey — — — — — — 
01826-02 O 1s 533.2 2.6 879 0.78 0.8 Contaminant 
01826-03 N 1s 401.0 1.4 9236 0.477 14.2 NH/NH2 
01826-03 N 1s 399.8 1.0 22755 0.477 34.9 –N=/NH 
01826-04d C 1s 287.5 1.3 15093 0.278 39.7 –N–C–N– (×4) 
01826-04d C 1s 286.0 1.0 3979 0.278 10.5 CN 
01827-01 Survey — — — — — — 
01827-02 Na 1s 1071.4 1.6 13661 1.685 4.2 Na+ 
01827-03 O 1s 532.8 1.5 20475 0.78 13.7 C–O–C, C–OH 
01827-03 O 1s 531.1 1.4 16995 0.78 11.4 ring C=O 
01827-04 N 1s 400.5 1.6 7417 0.477 8.1 NH/NH2 
01827-04 N 1s 399.1 1.3 6383 0.477 7.0 –N=/NH 
01827-05 C 1s 289.2 1.2 775 0.278 1.5 COO/N(CO)N 
01827-05 C 1s 287.9 1.5 5253 0.278 9.9 NCN/OCN 
01827-05 C 1s 286.6 1.4 12076 0.278 22.6 CN/CO 
01827-05 C 1s 285.0 1.2 9662 0.278 18.1 CH 
01827-06 P 2p 133.4 1.3 2219 0.486 3.6 –PO4– 
01827-06 P 2p — — 1109 0.486 — — 
01828-01 Survey — — — — — — 
01828-02 O 1s 533.1 1.6 23264 0.78 22.4 C–O–C, C–OH, 
01828-02 O 1s 531.5 1.4 9808 0.78 9.5 Ring C=O 
01828-03 N 1s 401.0 1.6 5916 0.477 9.3 NH/NH2 
01828-03 N 1s 399.7 1.5 3273 0.477 5.2 –N=/NH 
01828-04d C 1s 289.7 1.2 674 0.278 1.8 COO/N(CO)N 
01828-04d C 1s 288.2 1.6 4542 0.278 12.3 NCN/OCN 
01828-04d C 1s 286.8 1.3 10020 0.278 27.1 CN/CO 
01828-04d C 1s 285.0 1.2 3129 0.278 8.5 CH 
01828-05 P 2p 133.9 1.5 1698 0.486 3.9 –PO4– 
01828-05 P 2p — — 848 0.486 — — 
Spectrum ID #Element/transitionPeak energy (eV)Peak width FWHM (eV)Peak area (eV × cts/s)Sensitivity factorConcentration (at. %)Peak assignment
01822-01 Survey — — — — — — 
01822-02 O 1s 531.9 1.3 23366 0.78 21.9 Double bonded O 
01822-03 N 1s 400.8 1.2 15687 0.477 24.1 HN–C=O 
01822-04b C 1s 289.6 1.0 4990 0.278 13.1 –NH–(C=O)–NH– 
01822-04b C 1s 288.7 1.0 3880 0.278 10.2 –NH–(C=O)– 
01822-04b C 1s 286.7 1.1 5178 0.278 13.6 CN 
01822-04b C 1s 285.0 1.0 6472 0.278 17.0 CH 
01823-01 Survey … … … … … … 
01823-02 O 1s 531.7 1.3 19630 0.78 19.6 Double bonded O 
01823-03 N 1s 400.6 1.1 13732 0.477 22.4 HN–C=O 
01823-04b C 1s 289.4 1.0 4068 0.278 11.4 –NH–(C=O)–NH– 
01823-04b C 1s 288.3 1.0 3592 0.278 10.1 –NH–(C=O)– 
01823-04b C 1s 286.2 1.0 4259 0.278 11.9 CN 
01823-04b C 1s 285.0 1.0 8818 0.278 24.7 CH (x2) 
01824-01 Survey — — — — — — 
01824-02 Na 1s 1071.9 1.6 634 1.685 0.3 Na+ 
01824-03 O 1s 531.4 1.3 11044 0.78 11.3 double bonded O 
01824-04f N 1s 400.1 1.4 14657 0.477 24.5 –NH– 
01824-04f N 1s 399.0 1.2 7331 0.477 12.3 –N= 
01824-05c C 1s 288.8 1.1 5011 0.278 14.4 –NH–(C=O)–N= 
01824-05c C 1s 287.7 1.0 3891 0.278 11.2 –N–C–N– 
01824-05c C 1s 286.5 1.0 4207 0.278 12.1 CN 
01824-05c C 1s 285.0 1.1 4874 0.278 14.0 CH 
01825-01 Survey — — — — — — 
01825-02 O 1s 532.1 1.2 9024 0.78 8.2 Double bonded O 
01825-03 N 1s 401.0 1.4 15749 0.477 23.4 NH/NH2 
01825-03 N 1s 399.6 1.1 15614 0.477 23.2 –N=/NH 
01825-04 C 1s 288.8 1.0 6461 0.278 16.5 –NH–(C–NH2)–NH– & –NH–(C=O)– 
01825-04 C 1s 287.1 1.0 6866 0.278 17.5 –N–C–N– 
01825-04 C 1s 286.0 1.1 4404 0.278 11.2 CN 
01826-01 Survey — — — — — — 
01826-02 O 1s 533.2 2.6 879 0.78 0.8 Contaminant 
01826-03 N 1s 401.0 1.4 9236 0.477 14.2 NH/NH2 
01826-03 N 1s 399.8 1.0 22755 0.477 34.9 –N=/NH 
01826-04d C 1s 287.5 1.3 15093 0.278 39.7 –N–C–N– (×4) 
01826-04d C 1s 286.0 1.0 3979 0.278 10.5 CN 
01827-01 Survey — — — — — — 
01827-02 Na 1s 1071.4 1.6 13661 1.685 4.2 Na+ 
01827-03 O 1s 532.8 1.5 20475 0.78 13.7 C–O–C, C–OH 
01827-03 O 1s 531.1 1.4 16995 0.78 11.4 ring C=O 
01827-04 N 1s 400.5 1.6 7417 0.477 8.1 NH/NH2 
01827-04 N 1s 399.1 1.3 6383 0.477 7.0 –N=/NH 
01827-05 C 1s 289.2 1.2 775 0.278 1.5 COO/N(CO)N 
01827-05 C 1s 287.9 1.5 5253 0.278 9.9 NCN/OCN 
01827-05 C 1s 286.6 1.4 12076 0.278 22.6 CN/CO 
01827-05 C 1s 285.0 1.2 9662 0.278 18.1 CH 
01827-06 P 2p 133.4 1.3 2219 0.486 3.6 –PO4– 
01827-06 P 2p — — 1109 0.486 — — 
01828-01 Survey — — — — — — 
01828-02 O 1s 533.1 1.6 23264 0.78 22.4 C–O–C, C–OH, 
01828-02 O 1s 531.5 1.4 9808 0.78 9.5 Ring C=O 
01828-03 N 1s 401.0 1.6 5916 0.477 9.3 NH/NH2 
01828-03 N 1s 399.7 1.5 3273 0.477 5.2 –N=/NH 
01828-04d C 1s 289.7 1.2 674 0.278 1.8 COO/N(CO)N 
01828-04d C 1s 288.2 1.6 4542 0.278 12.3 NCN/OCN 
01828-04d C 1s 286.8 1.3 10020 0.278 27.1 CN/CO 
01828-04d C 1s 285.0 1.2 3129 0.278 8.5 CH 
01828-05 P 2p 133.9 1.5 1698 0.486 3.9 –PO4– 
01828-05 P 2p — — 848 0.486 — — 
a

Shading is added to facilitate grouping of data to corresponding spectrum.

b

FWHM restricted 1–4 eV in C 1s.

c

Fitted with FWHW 1–1.2 in C 1s.

d

FWHM restricted to 1–3 eV for C 1s.

e

FWHM restricted to 1–1.8 eV for C 1s.

f

For Peak Area, N fitted with area constraint 2:1.

ANALYZER CALIBRATION TABLE

Spectrum ID #Element/ TransitionPeak Energy (eV)Peak Width FWHM (eV)Peak Area (eV × cts/s)Sensitivity FactorConcentration (at. %)Peak Assignment
— Ag3d5/2 368.2 0.6 69083 5.987 100 Ag 
Spectrum ID #Element/ TransitionPeak Energy (eV)Peak Width FWHM (eV)Peak Area (eV × cts/s)Sensitivity FactorConcentration (at. %)Peak Assignment
— Ag3d5/2 368.2 0.6 69083 5.987 100 Ag 

GUIDE TO FIGURES

Spectrum (Accession) #Spectral regionVoltage shiftaMultiplierBaselineComment #
01822-01 Survey −3.303 — 
01822-02 O 1s −3.303 — 
01822-03 N 1s −3.303 — 
01822-04 C 1s −3.303 — 
01823-01 Survey −3.155 — 
01823-02 O 1s −3.155 — 
01823-03 N 1s −3.155 — 
01823-04 C 1s −3.155 — 
01824-01 Survey −3.628 — 
01824-02 Na 1s −3.628 — 
01824-03 O 1s −3.628 — 
01824-04 N 1s −3.628 — 
01824-05 C 1s −3.628 — 
01825-01 Survey −3.644 — 
01825-02 O 1s −3.644 — 
01825-03 N 1s −3.644 — 
01825-04 C 1s −3.644 — 
01826-01 Survey −3.831 — 
01826-02 O 1s −3.831 — 
01826-03 N 1s −3.831 — 
01826-04 C 1s −3.831 — 
01827-01 Survey −3.082 — 
01827-02 Na 1s −3.082 — 
01827-03 O 1s −3.082 — 
01827-04 N 1s −3.082 — 
01827-05 C 1s −3.082 — 
01827-06 P 2p −3.082 — 
01828-01 Survey −3.052 — 
01828-02 O 1s −3.052 — 
01828-03 N 1s −3.052 — 
01828-04 C 1s −3.052 — 
01828-05 P 2p −3.052 — 
Spectrum (Accession) #Spectral regionVoltage shiftaMultiplierBaselineComment #
01822-01 Survey −3.303 — 
01822-02 O 1s −3.303 — 
01822-03 N 1s −3.303 — 
01822-04 C 1s −3.303 — 
01823-01 Survey −3.155 — 
01823-02 O 1s −3.155 — 
01823-03 N 1s −3.155 — 
01823-04 C 1s −3.155 — 
01824-01 Survey −3.628 — 
01824-02 Na 1s −3.628 — 
01824-03 O 1s −3.628 — 
01824-04 N 1s −3.628 — 
01824-05 C 1s −3.628 — 
01825-01 Survey −3.644 — 
01825-02 O 1s −3.644 — 
01825-03 N 1s −3.644 — 
01825-04 C 1s −3.644 — 
01826-01 Survey −3.831 — 
01826-02 O 1s −3.831 — 
01826-03 N 1s −3.831 — 
01826-04 C 1s −3.831 — 
01827-01 Survey −3.082 — 
01827-02 Na 1s −3.082 — 
01827-03 O 1s −3.082 — 
01827-04 N 1s −3.082 — 
01827-05 C 1s −3.082 — 
01827-06 P 2p −3.082 — 
01828-01 Survey −3.052 — 
01828-02 O 1s −3.052 — 
01828-03 N 1s −3.052 — 
01828-04 C 1s −3.052 — 
01828-05 P 2p −3.052 — 
a

Voltage shift of the archived (as-measured) spectrum relative to the printed figure. The figure reflects the recommended energy scale correction due to a calibration correction, sample charging, flood gun, or other phenomenon.

Accession #: 01822-01 
■ Host material: Uracil 
■ Technique: XPS 
■ Spectral region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation source: Al Kα monochromatic 
Source energy: 1486.7 eV 
Source strength: 150 W 
Source size: 2  × 1 mm2 
Analyzer type: Spherical sector analyzer 
Incident angle: 54° 
Emission angle: 0° 
Analyzer pass energy: 160 eV 
Analyzer resolution: 2.4 eV 
Total signal accumulation time: 330 s 
Total elapsed time: ca 2061s 
Number of scans: 
Effective detector width: 33.6 eV 
Accession #: 01822-01 
■ Host material: Uracil 
■ Technique: XPS 
■ Spectral region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation source: Al Kα monochromatic 
Source energy: 1486.7 eV 
Source strength: 150 W 
Source size: 2  × 1 mm2 
Analyzer type: Spherical sector analyzer 
Incident angle: 54° 
Emission angle: 0° 
Analyzer pass energy: 160 eV 
Analyzer resolution: 2.4 eV 
Total signal accumulation time: 330 s 
Total elapsed time: ca 2061s 
Number of scans: 
Effective detector width: 33.6 eV 

Accession #: 01822-01 
■ Host material: Uracil 
■ Technique: XPS 
■ Spectral region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation source: Al Kα monochromatic 
Source energy: 1486.7 eV 
Source strength: 150 W 
Source size: 2  × 1 mm2 
Analyzer type: Spherical sector analyzer 
Incident angle: 54° 
Emission angle: 0° 
Analyzer pass energy: 160 eV 
Analyzer resolution: 2.4 eV 
Total signal accumulation time: 330 s 
Total elapsed time: ca 2061s 
Number of scans: 
Effective detector width: 33.6 eV 
Accession #: 01822-01 
■ Host material: Uracil 
■ Technique: XPS 
■ Spectral region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation source: Al Kα monochromatic 
Source energy: 1486.7 eV 
Source strength: 150 W 
Source size: 2  × 1 mm2 
Analyzer type: Spherical sector analyzer 
Incident angle: 54° 
Emission angle: 0° 
Analyzer pass energy: 160 eV 
Analyzer resolution: 2.4 eV 
Total signal accumulation time: 330 s 
Total elapsed time: ca 2061s 
Number of scans: 
Effective detector width: 33.6 eV 

Close modal

  • Accession #:01822-02

  • Host Material: Uracil

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 241 s

  • Total Elapsed Time: ca 1488 s

  • Number of Scans: 4

  • Effective Detector Width: 4.2 eV

  • Accession #:01822-02

  • Host Material: Uracil

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 241 s

  • Total Elapsed Time: ca 1488 s

  • Number of Scans: 4

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01822-03

  • Host Material: Uracil

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1488 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01822-03

  • Host Material: Uracil

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1488 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01822-04

  • Host Material: Uracil

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1488 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01822-04

  • Host Material: Uracil

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1488 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

Accession #:01823-01
■ Host Material: Thymine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Ka monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2064s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01823-01
■ Host Material: Thymine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Ka monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2064s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Accession #:01823-01
■ Host Material: Thymine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Ka monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2064s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01823-01
■ Host Material: Thymine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Ka monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2064s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Close modal

  • Accession #:01823-02

  • Host Material: Thymine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 241 s

  • Total Elapsed Time: ca 1490 s

  • Number of Scans: 4

  • Effective Detector Width: 4.2 eV

  • Accession #:01823-02

  • Host Material: Thymine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 241 s

  • Total Elapsed Time: ca 1490 s

  • Number of Scans: 4

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01823-03

  • Host Material: Thymine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1490 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01823-03

  • Host Material: Thymine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1490 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01823-04

  • Host Material: Thymine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2  × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1490 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01823-04

  • Host Material: Thymine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2  × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1490 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

Accession #:01824-01
■ Host Material: Cytosine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2134 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01824-01
■ Host Material: Cytosine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2134 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Accession #:01824-01
■ Host Material: Cytosine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2134 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01824-01
■ Host Material: Cytosine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2134 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Close modal

  • Accession #:01824-02

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: Na 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 603 s

  • Total Elapsed Time: ca 3078 s

  • Number of Scans: 10

  • Effective Detector Width: 4.2 eV

  • Accession #:01824-02

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: Na 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 603 s

  • Total Elapsed Time: ca 3078 s

  • Number of Scans: 10

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01824-03

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 302 s

  • Total Elapsed Time: ca 1561 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01824-03

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 302 s

  • Total Elapsed Time: ca 1561 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01824-04

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1561 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01824-04

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1561 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01824-05

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1561 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01824-05

  • Host Material: Cytosine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1561 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

Accession #: 01825-01 
■ Host Material: Guanine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2137 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #: 01825-01 
■ Host Material: Guanine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2137 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Accession #: 01825-01 
■ Host Material: Guanine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2137 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #: 01825-01 
■ Host Material: Guanine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2137 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Close modal

  • Accession #:01825-02

  • Host Material: Guanine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 302 s

  • Total Elapsed Time: ca 1563 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01825-02

  • Host Material: Guanine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 302 s

  • Total Elapsed Time: ca 1563 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01825-03

  • Host Material: Guanine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1563 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01825-03

  • Host Material: Guanine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 1563 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01825-04

  • Host Material: Guanine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1563 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01825-04

  • Host Material: Guanine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1563 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

Accession #:01826-01
■ Host Material: Adenine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2429 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01826-01
■ Host Material: Adenine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2429 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Accession #:01826-01
■ Host Material: Adenine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2429 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01826-01
■ Host Material: Adenine 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2429 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Close modal

  • Accession #:01826-02

  • Host Material: Adenine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 603 s

  • Total Elapsed Time: ca 1856 s

  • Number of Scans: 10

  • Effective Detector Width: 4.2 eV

  • Accession #:01826-02

  • Host Material: Adenine

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 603 s

  • Total Elapsed Time: ca 1856 s

  • Number of Scans: 10

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01826-03

  • Host Material: Adenine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 241 s

  • Total Elapsed Time: ca 1856s

  • Number of Scans: 4

  • Effective Detector Width: 4.2 eV

  • Accession #:01826-03

  • Host Material: Adenine

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 241 s

  • Total Elapsed Time: ca 1856s

  • Number of Scans: 4

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01826-04

  • Host Material: Adenine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 mm × 1 mm

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1856s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01826-04

  • Host Material: Adenine

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 mm × 1 mm

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1856s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

Accession #:01827-01
■ Host Material: DNA as Na salt 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 864 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01827-01
■ Host Material: DNA as Na salt 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 864 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Accession #:01827-01
■ Host Material: DNA as Na salt 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 864 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01827-01
■ Host Material: DNA as Na salt 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 864 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Close modal

  • Accession #:01827-02

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: Na 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Ka monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 181 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 3

  • Effective Detector Width: 4.2 eV

  • Accession #:01827-02

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: Na 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Ka monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 181 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 3

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01827-03

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 603 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 10

  • Effective Detector Width: 4.2 eV

  • Accession #:01827-03

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 603 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 10

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01827-04

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2  × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01827-04

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2  × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01827-05

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

  • Accession #:01827-05

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 301 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 5

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01827-06

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: P 2p

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 423 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01827-06

  • Host Material: DNA

  • Technique: XPS

  • Spectral Region: P 2p

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 423 s

  • Total Elapsed Time: ca 2622 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

Accession #:01828-01
■ Host Material: RNA 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2135 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01828-01
■ Host Material: RNA 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2135 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Accession #:01828-01
■ Host Material: RNA 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2135 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 
Accession #:01828-01
■ Host Material: RNA 
■ Technique: XPS 
■ Spectral Region: Survey 
Instrument: Kratos Axis Ultra DLD 
Excitation Source: Al Kα monochromatic 
Source Energy: 1486.7 eV 
Source Strength: 150 W 
Source Size: 2 × 1 mm2 
Analyzer Type: Spherical sector analyzer 
Incident Angle: 54° 
Emission Angle: 0° 
Analyzer Pass Energy: 160 eV 
Analyzer Resolution: 2.4 eV 
Total Signal Accumulation Time: 330 s 
Total Elapsed Time: ca 2135 s 
Number of Scans: 
Effective Detector Width: 33.6 eV 

Close modal

  • Accession #:01828-02

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 181 s

  • Total Elapsed Time: ca 1562 s

  • Number of Scans: 3

  • Effective Detector Width: 4.2 eV

  • Accession #:01828-02

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: O 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 181 s

  • Total Elapsed Time: ca 1562 s

  • Number of Scans: 3

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01828-03

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1562 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01828-03

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: N 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1562 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01828-04

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1562 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

  • Accession #:01828-04

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: C 1s

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 422 s

  • Total Elapsed Time: ca 1562 s

  • Number of Scans: 7

  • Effective Detector Width: 4.2 eV

Close modal

  • Accession #:01828-05

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: P 2p

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 906 s

  • Total Elapsed Time: ca 3449 s

  • Number of Scans: 15

  • Effective Detector Width: 4.2 eV

  • Accession #:01828-05

  • Host Material: RNA

  • Technique: XPS

  • Spectral Region: P 2p

  • Instrument: Kratos Axis Ultra DLD

  • Excitation Source: Al Kα monochromatic

  • Source Energy: 1486.7 eV

  • Source Strength: 150 W

  • Source Size: 2 × 1 mm2

  • Analyzer Type: Spherical sector

  • Incident Angle: 54°

  • Emission Angle: 0°

  • Analyzer Pass Energy 20 eV

  • Analyzer Resolution: 0.3 eV

  • Total Signal Accumulation Time: 906 s

  • Total Elapsed Time: ca 3449 s

  • Number of Scans: 15

  • Effective Detector Width: 4.2 eV

Close modal

Funding for XPS analyses was received from the Kempe foundation in Sweden by MR (JCK-1720).

The authors have no conflicts to disclose.

Madeleine Ramstedt: Conceptualization (lead); Data curation (equal); Formal analysis (equal); Funding acquisition (lead); Investigation (equal); Methodology (equal); Project administration (lead); Resources (equal); Validation (equal); Visualization (lead); Writing – original draft (lead); Writing – review & editing (equal). Andrey Shchukarev: Conceptualization (supporting); Data curation (equal); Formal analysis (equal); Investigation (equal); Methodology (equal); Resources (equal); Validation (equal); Visualization (supporting); Writing – original draft (supporting); Writing – review & editing (equal).

The raw data that support the findings of this study are available within the article and its supplementary material.

1.
A.
Shchukarev
,
E.
Backman
,
S.
Watts
,
S.
Salentinig
,
C. F.
Urban
, and
M.
Ramstedt
,
Front. Chem.
9
, 1 (
2021
).
2.
G.
Sun
and
M. C.
Nicklaus
,
Theor. Chem. Acc.
117
,
323
(
2007
).
3.
R. P.
Bowater
and
A.
Gates
,
eLS
2015, 1.
4.
S.
Ptasinska
,
A.
Stypczynska
,
T.
Nixon
,
N. J.
mason
,
D. V.
Klyachko
, and
L.
Sanche
,
J. Chem. Phys.
129
,
065102
(
2008
).
5.
C. J.
May
,
H. E.
Canavan
, and
D. G.
Castner
,
Anal. Chem.
76
,
1114
(
2004
).
6.
J.
Pelling
,
F. E.
Hruska
, and
N. S.
McIntyre
,
Can. J. Chem.
56
,
1555
(
1978
).
7.
C. D. Wagner, L. E. Davis, M. V. Zeller, J. A. Taylor, R. H. Raymond, L. H. Gale,
Surf. Interface Anal.
3
, 211 (1981).

Supplementary Material