Cryogenic x-ray photoelectron spectroscopy was used to analyze the cell envelope of intact and hydrated Gram-negative bacteria of the species Pseudomonas fluorescens. We used a reference strain, DSM50090, from the German microbial culture collection, which we previously have suggested would function well as a reference strain for future XPS analyses of Gram-negative bacteria. Bacteria were grown on nutrient agar plates at room temperature, collected with a cultivation loop, and washed using phosphate buffered saline. An aliquot of the cell pellet was fast-frozen on the sample holder in the sample introduction chamber to a temperature of 103 K and kept frozen throughout the measurement. Survey spectra and high-resolution spectra of Na 1s, O 1s, N 1s, C 1s, Cl 2p, S 2p, and P 2p are reported. The spectra obtained from the analyzed cells represent a combined signal from O, N, C, and S atoms in proteins, lipids, and polysaccharides at the cell surface. Furthermore, signal from P, Na, K, and Cl atoms was present both originating from processes in the cell envelope and remnants from the wash buffer.
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June 2022
Research Article|
March 23 2022
Cryo-XPS spectra from bacterial reference strain Pseudomonas fluorescens DSM50090
Madeleine Ramstedt
;
Madeleine Ramstedt
a)
Department of Chemistry, Umeå University
, 901 87 Umeå, Sweden
a)Author to whom correspondence should be addressed: madeleine.ramstedt@umu.se
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Andrey Shchukarev
Andrey Shchukarev
b)
Department of Chemistry, Umeå University
, 901 87 Umeå, Sweden
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a)Author to whom correspondence should be addressed: madeleine.ramstedt@umu.se
b)
Electronic mail: andrey.shchukarev@umu.se
Surf. Sci. Spectra 29, 014010 (2022)
Article history
Received:
October 21 2021
Accepted:
February 25 2022
Citation
Madeleine Ramstedt, Andrey Shchukarev; Cryo-XPS spectra from bacterial reference strain Pseudomonas fluorescens DSM50090. Surf. Sci. Spectra 1 June 2022; 29 (1): 014010. https://doi.org/10.1116/6.0001575
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