Near-ambient pressure x-ray photoelectron spectroscopy (NAP-XPS) is a less traditional form of XPS that allows samples to be analyzed at relatively high pressures, i.e., at greater than 2500 Pa. With NAP-XPS, XPS can probe moderately volatile liquids, biological samples, porous materials, and/or polymeric materials that outgas significantly. In this submission, we show survey, C 1s, O 1s, and N 1s narrow scans from an aqueous solution of a common protein, bovine serum albumin. The C 1s peak envelope is well fit to four symmetric peaks of equal width that correspond to carbon bonded to carbon and hydrogen (C-1), carbon singly bonded to oxygen (C-2), carbonyl and/or amide carbon (C-3), and carboxyl carbon (C-4). Two possible peak fits are considered for the N 1s and O 1s peak envelopes. The N 1s signal is fit to four peaks that correspond to amine (—NH2), amide (O˭C‒NH2), ammonium (—NH3+), and N2(g) nitrogen, and alternatively to three peaks that correspond to amine, amide, and N2(g) nitrogen. The O 1s peak envelope is similarly fit to three and four components.
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June 2019
Research Article|
July 18 2019
Bovine serum albumin, aqueous solution, by near-ambient pressure XPS
Varun Jain;
Varun Jain
1
Department of Chemistry and Biochemistry, Brigham Young University
, C100 BNSN, Provo, Utah 84602
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Marit Kjærvik;
Marit Kjærvik
2
Division 6.1 Surface Analysis and Interfacial Chemistry, Bundesanstalt für Materialforschung und –prüfung
, Unter den Eichen 44-46, Berlin 12203, Germany
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Stephan Bahr;
Stephan Bahr
3
SPECS Surface Nano Analysis GmbH
, Voltastrasse 5, 13355 Berlin, Germany
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Paul Dietrich
;
Paul Dietrich
3
SPECS Surface Nano Analysis GmbH
, Voltastrasse 5, 13355 Berlin, Germany
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Michael Meyer;
Michael Meyer
3
SPECS Surface Nano Analysis GmbH
, Voltastrasse 5, 13355 Berlin, Germany
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Andreas Thißen;
Andreas Thißen
3
SPECS Surface Nano Analysis GmbH
, Voltastrasse 5, 13355 Berlin, Germany
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Matthew R. Linford
Matthew R. Linford
a)
1
Department of Chemistry and Biochemistry, Brigham Young University
, C100 BNSN, Provo, Utah 84602
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a)
Electronic mail: mrlinford@byu.edu
Surf. Sci. Spectra 26, 014027 (2019)
Article history
Received:
September 06 2018
Accepted:
June 12 2019
Citation
Varun Jain, Marit Kjærvik, Stephan Bahr, Paul Dietrich, Michael Meyer, Andreas Thißen, Matthew R. Linford; Bovine serum albumin, aqueous solution, by near-ambient pressure XPS. Surf. Sci. Spectra 1 June 2019; 26 (1): 014027. https://doi.org/10.1116/1.5055229
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