This study investigated a SARS-CoV-2 virus detection mechanism using dry-stored disposable strips. The accuracy of this sensing platform is as good as polymerase chain reaction (PCR) with a detection time of fewer than 30 s. SARS-CoV-2 antibodies were biofunctionalized on disposable strips similar to glucose detection strips to detect the presence and concentrations of SARS-CoV-2 in saliva samples. Eight 1 ms electric pulses were sent through the sensor strip with a saliva sample in its microfluidic chamber. A circuit board embedded with MOSFET was also employed to amplify the detected signals and convert the signal to digital readings displayed on an LCD screen. The COV-antibody functionalized disposable strips were stored in a dry condition for at least one day before analyzing clinical human saliva samples with known cycling threshold (Ct) values confirmed with conventional PCR tests. Results demonstrate our system is capable of showing qualitative positive or negative results within 30 s and providing quantitative SARS-CoV-2 concentrations in terms of Ct values in 5 min.

1.
G.
Rong
,
Y.
Zheng
,
Y.
Chen
,
Y.
Zhang
,
P.
Zhu
, and
M.
Sawan
, “Encyclopedia of sensors and biosensors,” 3, 17 (2023).
2.
M.
Shen
,
Y.
Zhou
,
J.
Ye
,
A. A.
Abdullah Al-Maskri
,
Y.
Kang
,
S.
Zeng
, and
S.
Cai
,
J. Pharm. Anal.
10
,
97
(
2020
).
3.
B.
Udugama
 et al.,
ACS Nano
14
,
3822
(
2020
).
4.
M.
Platten
,
D.
Hoffmann
,
R.
Grosser
,
F.
Wisplinghoff
,
H.
Wisplinghoff
,
G.
Wiesmüller
,
O.
Schildgen
, and
V.
Schildgen
,
Viruses
13
, 1459 (
2021
).
5.
D.
Sung
and
J.
Koo
,
Biomed. Eng. Lett.
11
,
85
(
2021
).
7.
J.
Li
,
D.
Wu
,
Y.
Yu
,
T.
Li
,
K.
Li
,
M. M.
Xiao
,
Y.
Li
,
Z. Y.
Zhang
, and
G. J.
Zhang
,
Biosens. Bioelectron.
183
,
113206
(
2021
).
8.
L.
Wang
 et al.,
Nat. Biomed. Eng.
6
,
276
(
2022
).
9.
S.
Kumar
,
R. K.
Chauhan
, and
M.
Kumar
,
Silicon
14, 11453 (
2022
).
10.
M.
Xian
 et al.,
J. Vac. Sci. Technol. B
39
,
033202
(
2021
).
11.
S. Y.
Lu
,
S. S.
Shan
,
J.
Yang
,
C. W.
Chang
,
F.
Ren
,
J.
Lin
,
S.
Pearton
, and
Y. T.
Liao
, 2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Berlin, Germany, 23–27 July 2019 (
IEEE
,
New York
,
2019
), p.
5761
.
12.
P. H.
Carey
,
J.
Yang
,
F.
Ren
,
C.-W.
Chang
,
J.
Lin
,
S. J.
Pearton
,
B.
Lobo
, and
M. E.
Leon
,
J. Electrochem. Soc.
166
,
B708
(
2019
).
13.
J.
Yang
,
P.
Carey
,
F.
Ren
,
Y.-L.
Wang
,
M. L.
Good
,
S.
Jang
,
M. A.
Mastro
, and
S. J.
Pearton
,
Appl. Phys. Lett.
111
,
202104
(
2017
).
14.
J.
Yang
,
P.
Carey
,
F.
Ren
,
M. A.
Mastro
,
K.
Beers
,
S. J.
Pearton
, and
I. I.
Kravchenko
,
Appl. Phys. Lett.
113
,
032101
(
2018
).
15.
C.-W.
Chiu
 et al.,
J. Vac. Sci. Technol. B
40
,
023204
(
2022
).
You do not currently have access to this content.