The ability to harness the processes by which complex tissues arise during embryonic development would improve the ability to engineer complex tissuelike constructs in vitro—a longstanding goal of tissue engineering and regenerative medicine. In embryos, uniform populations of stem cells are exposed to spatial gradients of diffusible extracellular signaling proteins, known as morphogens. Varying levels of these signaling proteins induce stem cells to differentiate into distinct cell types at different positions along the gradient, thus creating spatially patterned tissues. Here, the authors describe two straightforward and easy-to-adopt microfluidic strategies to expose human pluripotent stem cells in vitro to spatial gradients of desired differentiation-inducing extracellular signals. Both approaches afford a high degree of control over the distribution of extracellular signals, while preserving the viability of the cultured stem cells. The first microfluidic platform is commercially available and entails static culture, whereas the second microfluidic platform requires fabrication and dynamic fluid exchange. In each platform, the authors first computationally modeled the spatial distribution of differentiation-inducing extracellular signals. Then, the authors used each platform to expose human pluripotent stem cells to a gradient of these signals (in this case, inducing a cell type known as the primitive streak), resulting in a regionalized culture with differentiated primitive streak cells predominately localized on one side and undifferentiated stem cells at the other side of the device. By combining this approach with a fluorescent reporter for differentiated cells and live-cell fluorescence imaging, the authors characterized the spatial and temporal dynamics of primitive streak differentiation within the induced signaling gradients. Microfluidic approaches to create precisely controlled morphogen gradients will add to the stem cell and developmental biology toolkit, and may eventually pave the way to create increasingly spatially patterned tissuelike constructs in vitro.

1.
K. W.
Rogers
and
A. F.
Schier
,
Annu. Rev. Cell Dev. Biol.
27
,
377
(
2011
).
2.
J.
Briscoe
and
S.
Small
,
Development
142
,
3996
(
2015
).
3.
S.
Shimozono
,
T.
Iimura
,
T.
Kitaguchi
,
S.-i.
Higashijima
, and
A.
Miyawaki
,
Nature
496
,
363
(
2013
).
4.
S. R.
Yu
,
M.
Burkhardt
,
M.
Nowak
,
J.
Ries
,
Z.
Petrášek
,
S.
Scholpp
,
P.
Schwille
, and
M.
Brand
,
Nature
461
,
533
(
2009
).
5.
M.
Zagorski
,
Y.
Tabata
,
N.
Brandenberg
,
M. P.
Lutolf
,
G.
Tkačik
,
T.
Bollenbach
,
J.
Briscoe
, and
A.
Kicheva
,
Science
356
,
1379
(
2017
).
6.
C. J.
Demers
,
P.
Soundararajan
,
P.
Chennampally
,
G. A.
Cox
,
J.
Briscoe
,
S. D.
Collins
, and
R. L.
Smith
,
Development
143
,
1884
(
2016
).
7.
A.
Manfrin
,
Y.
Tabata
,
E. R.
Paquet
,
A. R.
Vuaridel
,
F. R.
Rivest
,
F.
Naef
, and
M. P.
Lutolf
,
Nat. Methods
16
,
640
(
2019
).
8.
J. A.
Thomson
,
J.
Itskovitz-Eldor
,
S. S.
Shapiro
,
M. A.
Waknitz
,
J. J.
Swiergiel
,
V. S.
Marshall
, and
J. M.
Jones
,
Science
282
,
1145
(
1998
).
9.
K.
Takahashi
,
K.
Tanabe
,
M.
Ohnuki
,
M.
Narita
,
T.
Ichisaka
,
K.
Tomoda
, and
S.
Yamanaka
,
Cell
131
,
861
(
2007
).
10.
K. M.
Loh
et al.,
Cell Stem Cell
14
,
237
(
2014
).
11.
K. M.
Loh
et al.,
Cell
166
,
451
(
2016
).
12.
J. L.
Fowler
,
L. T.
Ang
, and
K. M.
Loh
,
Wiley Interdiscip. Rev. Develop. Biol.
e368
, (
2019
).
13.
C.
Davenport
,
U.
Diekmann
,
I.
Budde
,
N.
Detering
, and
O.
Naujok
,
Stem Cells
34
,
2635
(
2016
).
14.
A.
Kirkeby
,
S.
Grealish
,
D. A.
Wolf
,
J.
Nelander
,
J.
Wood
,
M.
Lundblad
,
O.
Lindvall
, and
M.
Parmar
,
Cell Rep.
1
,
703
(
2012
).
15.
G. M.
Whitesides
,
Nature
442
,
368
(
2006
).
16.
S.
Takayama
,
J. C.
McDonald
,
E.
Ostuni
,
M. N.
Liang
,
P. J.
Kenis
,
R. F.
Ismagilov
, and
G. M.
Whitesides
,
Proc. Natl. Acad. Sci. U.S.A.
96
,
5545
(
1999
).
17.
E.
Cimetta
,
A.
Godier-Furnémont
, and
G.
Vunjak-Novakovic
,
Curr. Opin. Biotechnol.
24
,
926
(
2013
).
18.
E.
Cimetta
and
G.
Vunjak-Novakovic
,
Exp. Biol. Med.
239
,
1255
(
2014
).
19.
S.
Halldorsson
,
E.
Lucumi
,
R.
Gómez-Sjöberg
, and
R. M.
Fleming
,
Biosens. Bioelectron.
63
,
218
(
2015
).
20.
E. K.
Sackmann
,
A. L.
Fulton
, and
D. J.
Beebe
,
Nature
507
,
181
(
2014
).
21.
G. M.
Whitesides
and
A. D.
Stroock
,
Phys. Today
54
(
6
),
42
(
2001
).
22.
J. C.
McDonald
and
G. M.
Whitesides
,
Acc. Chem. Res.
35
,
491
(
2002
).
23.
G.
Velve-Casquillas
,
M.
Le Berre
,
M.
Piel
, and
P. T.
Tran
,
Nano Today
5
,
28
(
2010
).
24.
J. N.
Tan
and
A.
Neild
,
AIP Adv.
2
,
032160
(
2012
).
25.
D. I.
Walsh
III,
D. S.
Kong
,
S. K.
Murthy
, and
P. A.
Carr
,
Trends Biotechnol.
35
,
383
(
2017
).
26.
R. D.
Sochol
,
E.
Sweet
,
C. C.
Glick
,
S.-Y.
Wu
,
C.
Yang
,
M.
Restaino
, and
L.
Lin
,
Microelectron. Eng.
189
,
52
(
2018
).
27.
A. E.
Kamholz
,
B. H.
Weigl
,
B. A.
Finlayson
, and
P.
Yager
,
Anal. Chem.
71
,
5340
(
1999
).
28.
N. L.
Jeon
,
S. K.
Dertinger
,
D. T.
Chiu
,
I. S.
Choi
,
A. D.
Stroock
, and
G. M.
Whitesides
,
Langmuir
16
,
8311
(
2000
).
29.
H.-W.
Wu
,
C.-C.
Lin
, and
G.-B.
Lee
,
Biomicrofluidics
5
,
013401
(
2011
).
30.
L. G.
Villa-Diaz
,
Y.-s.
Torisawa
,
T.
Uchida
,
J.
Ding
,
N. C.
Nogueira-de Souza
,
K. S.
O’Shea
,
S.
Takayama
, and
G. D.
Smith
,
Lab Chip
9
,
1749
(
2009
).
31.
E. M.
Lucchetta
,
J. H.
Lee
,
L. A.
Fu
,
N. H.
Patel
, and
R. F.
Ismagilov
,
Nature
434
,
1134
(
2005
).
32.
W.-T.
Fung
,
A.
Beyzavi
,
P.
Abgrall
,
N.-T.
Nguyen
, and
H.-Y.
Li
,
Lab Chip
9
,
2591
(
2009
).
33.
J.
Melin
and
S. R.
Quake
,
Annu. Rev. Biophys. Biomol. Struct.
36
,
213
(
2007
).
34.
M.
Cabello
et al.,
Sens. Actuators B Chem.
288
,
337
(
2019
).
35.
M. A.
Unger
,
H.-P.
Chou
,
T.
Thorsen
,
A.
Scherer
, and
S. R.
Quake
,
Science
288
,
113
(
2000
).
36.
P.
Zengel
,
A.
Nguyen-Hoang
,
C.
Schildhammer
,
R.
Zantl
,
V.
Kahl
, and
E.
Horn
,
BMC Cell Biol.
12
,
21
(
2011
).
37.
R.
Zantl
and
E.
Horn
, “Chemotaxis of slow migrating mammalian cells analysed by video microscopy,” in Cell Migration (Springer, New York, 2011), pp. 191–203.
38.
R. P.
Davis
,
E. S.
Ng
,
M.
Costa
,
A. K.
Mossman
,
K.
Sourris
,
A. G.
Elefanty
, and
E. G.
Stanley
,
Blood
111
,
1876
(
2008
).
39.
H.
Fischer
,
I.
Polikarpov
, and
A. F.
Craievich
,
Protein Sci.
13
,
2825
(
2004
).
40.
L.
He
and
B.
Niemeyer
,
Biotechnol. Prog.
19
,
544
(
2003
),
41.
C.
Wang
,
H.
Lu
, and
M. A.
Schwartz
,
J. Biomech.
45
,
1212
(
2012
).
42.
K. V.
Christ
,
K. B.
Williamson
,
K. S.
Masters
, and
K. T.
Turner
,
Biomed. Microdevices
12
,
443
(
2010
).
43.
N. T.
Kohen
,
L. E.
Little
, and
K. E.
Healy
,
Biointerphases
4
,
69
(
2009
).
44.
L.
Przybyla
and
J.
Voldman
,
Annu. Rev. Anal. Chem.
5
,
293
(
2012
),
45.
See supplementary material at http://dx.doi.org/10.1116/1.5142012 for time-lapse imaging experiments.

Supplementary Material

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