Textured surfaces obtained by UV laser ablation of poly(ethylene terephthalate) films were used to study the effect of shape and spacing of surface features on cellular response. Two distinct patterns, cones and ripples with spacing from 2 to 25 μm, were produced. Surface features with different shapes and spacings were produced by varying pulse repetition rate, laser fluence, and exposure time. The effects of the surface texture parameters, i.e., shape and spacing, on cell attachment, proliferation, and morphology of neonatal human dermal fibroblasts and mouse fibroblasts were studied. Cell attachment was the highest in the regions with cones at ≈4 μm spacing. As feature spacing increased, cell spreading decreased, and the fibroblasts became more circular, indicating a stress-mediated cell shrinkage. This study shows that UV laser ablation is a useful alternative to lithographic techniques to produce surface patterns for controlling cell attachment and growth on biomaterial surfaces.
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Research Article|
June 02 2010
UV laser-ablated surface textures as potential regulator of cellular response
Prafulla Chandra;
Prafulla Chandra
1New Jersey Center for Biomaterials,
Rutgers University
, 145 Bevier Road, Piscataway, New Jersey 08854, USA
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Karen Lai;
Karen Lai
1New Jersey Center for Biomaterials,
Rutgers University
, 145 Bevier Road, Piscataway, New Jersey 08854, USA
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Hak-Joon Sung;
Hak-Joon Sung
1New Jersey Center for Biomaterials,
Rutgers University
, 145 Bevier Road, Piscataway, New Jersey 08854, USA
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N. Sanjeeva Murthy;
N. Sanjeeva Murthy
1New Jersey Center for Biomaterials,
Rutgers University
, 145 Bevier Road, Piscataway, New Jersey 08854, USA
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Joachim Kohn
Joachim Kohn
a)
1New Jersey Center for Biomaterials,
Rutgers University
, 145 Bevier Road, Piscataway, New Jersey 08854, USA
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Prafulla Chandra
1
Karen Lai
1
Hak-Joon Sung
1
N. Sanjeeva Murthy
1
Joachim Kohn
1,a)
1New Jersey Center for Biomaterials,
Rutgers University
, 145 Bevier Road, Piscataway, New Jersey 08854, USA
a)
Author to whom correspondence should be addressed; electronic mail: [email protected]
Biointerphases 5, 53–59 (2010)
Article history
Received:
March 30 2010
Accepted:
May 05 2010
Citation
Prafulla Chandra, Karen Lai, Hak-Joon Sung, N. Sanjeeva Murthy, Joachim Kohn; UV laser-ablated surface textures as potential regulator of cellular response. Biointerphases 1 June 2010; 5 (2): 53–59. https://doi.org/10.1116/1.3438080
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