The phase behavior and lateral organization of saturated phosphatidylethanolamine (PE) and phosphatidylcholine (PC) bilayers were investigated using atomic force microscopy (AFM) and force-volume (FV) imaging for both pure and two component mixed layers. The results demonstrated the existence of unexpected segregated domains in pure PE membranes at temperatures well below the transition temperature (Tm) of the component phospholipid. These domains were of low mechanical stability and lacked the capacity for hydrogen bonding between lipid headgroups. Temperature dependent studies for different PC/PE ratios using AFM also demonstrated the mixing of these phospholipid bilayers to exhibit only a single gel to liquid transition temperature. Further work performed using FV imaging and chemically modified probes established that no lipid segregation exists at the PC/PE ratios investigated.
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See EPAPS Document No. E-BJIOBN-3-001804 for a complete data set for temperature dependent studies for DMPE:DMPC binary supported bilayers. Histograms of adhesive forces measured for carboxy-modified AFM tips and the LDs of pure and binary DMPE phospholipid bilayers are also presented. For more information on EPAPS, see http:// www.aip.org/pubservs/epaps.html
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