We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes. Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle. PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode. A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced. The viscoelastic properties of adsorbed protein, characterized using the ΔDf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings. Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.

1.
G. C.
Gurtner
,
S.
Werner
,
Y.
Barrandon
, and
M. T.
Longaker
,
Nature
453
,
314
(
2008
).
2.
M.
Talikowska
,
X.
Fu
, and
G.
Lisak
,
Biosens. Bioelectron.
135
,
50
(
2019
).
4.
S.
Das
and
A. B.
Baker
,
Front. Bioeng. Biotechnol.
4
,
82
(
2016
).
5.
S.
Aarabi
,
M. T.
Longaker
, and
G. C.
Gurtner
,
PLoS Med.
4
,
e234
(
2007
).
6.
S.
Dhivya
,
V. V.
Padma
, and
E.
Santhini
,
Biomedicine
5
,
22
(
2015
).
7.
J. S.
Boateng
,
K. H.
Matthews
,
H. N. E.
Stevens
, and
G. M.
Eccleston
,
J. Pharm. Sci.
97
,
2892
(
2008
).
8.
J. J.
Hutchinson
and
M.
McGuckin
,
Am. J. Infect. Control
18
,
257
(
1990
).
9.
G. A.
Kannon
and
A. B.
Garrett
,
Dermatol. Surg.
21
,
583
(
1995
).
10.
T. M.
Baum
and
M. J.
Busuito
,
Adv Wound Care
11
,
40
(
1998
).
11.
Q.
Gu
,
S.
Wang
,
Q.
Wang
, and
X.
Wei
,
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
28
,
255
(
2014
).
12.
M.
Mian
,
F.
Beghe
, and
E.
Mian
,
Int. J. Tissue React.
14
,
1
(
1992
).
13.
M.
Prosdocimi
and
C.
Bevilacqua
,
Panminerva Med.
54
,
129
(
2012
).
14.
Z.
Ruszczak
,
Adv. Drug Delivery Rev.
55
,
1595
(
2003
).
15.
R. M.
Farahani
and
L. C.
Kloth
,
Int. Wound J.
5
,
477
(
2008
).
16.
S.
Ud-Din
and
A.
Bayat
,
Healthcare
2
,
445
(
2014
).
17.
J.
Boateng
and
O.
Catanzano
,
J. Pharm. Sci.
104
,
3653
(
2015
).
18.
G.
Wallace
and
G.
Spinks
,
Soft Matter
3
,
665
(
2007
).
19.
E.
Stewart
,
N. R.
Kobayashi
,
M. J.
Higgins
,
A. F.
Quigley
,
S.
Jamali
,
S. E.
Moulton
,
R. M.
Kapsa
,
G. G.
Wallace
, and
J. M.
Crook
,
Tissue Eng. Part C
21
,
385
(
2014
).
20.
P
. J.
Molino
,
Z.
Yue
,
B.
Zhang
,
A.
Tibbens
,
X.
Liu
,
R. M. I.
Kapsa
,
M. J.
Higgins
, and
G. G.
Wallace
,
Adv. Mater. Interfaces
1
,
1300122
(
2014
).
21.
K.
Svennersten
,
M.
Berggren
,
A.
Richter-Dahlfors
, and
E. W. H.
Jager
,
Lab Chip
11
,
3287
(
2011
).
22.
B. C.
Thompson
,
R. T.
Richardson
,
S. E.
Moulton
,
A. J.
Evans
,
S.
O'Leary
,
G. M.
Clark
, and
G. G.
Wallace
,
J. Controlled Release
141
,
161
(
2010
).
23.
P. J.
Molino
,
M. J.
Higgins
,
P. C.
Innis
,
R. M. I.
Kapsa
, and
G. G.
Wallace
,
Langmuir
28
,
8433
(
2012
).
24.
A. M. D.
Wan
,
D. J.
Brooks
,
A.
Gumus
,
C.
Fischbach
, and
G. G.
Malliaras
,
Chem. Commun.
2009
,
5278
.
25.
A.
Gumus
,
J. P.
Califano
,
A. M. D.
Wan
,
J.
Huynh
,
C. A.
Reinhart-King
, and
G. G.
Malliaras
,
Soft Matter
6
,
5138
(
2010
).
26.
R. A. F.
Clark
,
Ann. N. Y. Acad. Sci.
936
,
355
(
2001
).
27.
D.
Brett
,
Wounds
20
,
347
(
2008
).
28.
M. F.
Carlevaro
,
A.
Albini
,
D.
Ribatti
,
C.
Gentili
,
R.
Benelli
,
S.
Cermelli
,
R.
Cancedda
, and
F. D.
Cancedda
,
J. Cell Biol.
136
,
1375
(
1997
).
29.
A.
Gelmi
,
M. J.
Higgins
, and
G. G.
Wallace
,
Small
9
,
393
(
2013
).
30.
P. J.
Molino
,
A.
Tibbens
,
R. M. I.
Kapsa
, and
G. G.
Wallace
,
MRS Proc.
1569
,
225
(
2013
).
31.
P. J.
Molino
,
L.
Garcia
,
E. M.
Stewart
,
M.
Lamaze
,
B.
Zhang
,
A. R.
Harris
,
P.
Winberg
, and
G. G.
Wallace
,
Biomater. Sci.
6
,
1250
(
2018
).
32.
R. A.
Green
,
N. H.
Lovell
, and
L. A.
Poole-Warren
,
Acta Biomater.
6
,
63
(
2010
).
33.
K. J.
Gilmore
,
M.
Kita
,
Y.
Han
,
A.
Gelmi
,
M. J.
Higgins
,
S. E.
Moulton
,
G. M.
Clark
,
R.
Kapsa
, and
G. G.
Wallace
,
Biomaterials
30
,
5292
(
2009
).
34.
B.
Zhang
,
P. J.
Molino
,
A. R.
Harris
,
Z.
Yue
,
S. E.
Moulton
, and
G. G.
Wallace
,
J. Mater. Chem. B
4
,
2570
(
2016
).
35.
R.
Muller
,
Z.
Yue
,
S.
Ahmadi
,
W.
Ng
,
W. M.
Grosse
,
M. J.
Cook
,
G. G.
Wallace
, and
S. E.
Moulton
,
Sens. Actuators B
236
,
732
(
2016
).
36.
S.
Meng
,
M.
Rouabhia
,
G.
Shi
, and
Z.
Zhang
,
J. Biomed. Mater. Res., Part A
87
,
332
(
2008
).
37.
J. H.
Collier
,
J. P.
Camp
,
T. W.
Hudson
, and
C. E.
Schmidt
,
J. Biomed. Mater. Res.
50
,
574
(
2000
).
38.
M.
Rouabhia
,
H.
Park
,
S.
Meng
,
H.
Derbali
, and
Z.
Zhang
,
Plos One
8
,
e71660
(
2013
).
39.
Y.
Wang
,
M.
Rouabhia
,
D.
Lavertu
, and
Z.
Zhang
,
J. Tissue Eng. Regen. Med.
11
,
1110
(
2015
).
40.
A. M.-D.
Wan
 et al,
J. Mater. Chem. B
3
,
5040
(
2015
).
41.
M.
Lahaye
and
A.
Robic
,
Biomacromolecules
8
,
1765
(
2007
).
42.
F.
Chiellini
,
A.
Morelli
,
F.
Chiellini
, and
A.
Morelli
,
Biomaterials: Physics and Chemistry
, edited by R. Pignatello (InTech, Croatia, 2011), pp. 75–98.
43.
W.
Mao
,
X.
Zang
,
Y.
Li
, and
H.
Zhang
,
J. Appl. Phycol.
18
,
9
(
2006
).
44.
V.
Gadenne
,
L.
Lebrun
,
T.
Jouenne
, and
P.
Thebault
,
Colloids Surf. B
112
,
229
(
2013
).
45.
X.
Chen
,
Z.
Yue
,
P. C.
Winberg
,
J. N.
Dinoro
,
P.
Hayes
,
S.
Beirne
, and
G. G.
Wallace
,
Biomater. Sci.
7
,
3497
(
2019
).
46.
L.
Kang
,
X.
Liu
,
Z.
Yue
,
Z.
Chen
,
C.
Baker
,
C. P.
Winberg
, and
G. G.
Wallace
,
Polymers
10
,
415
(
2018
).
47.
J.
Malmström
,
H.
Agheli
,
P.
Kingshott
, and
D. S.
Sutherland
,
Langmuir
23
,
9760
(
2007
).
48.
M. B.
Hovgaard
,
K.
Rechendorff
,
J.
Chevallier
,
M.
Foss
, and
F.
Besenbacher
,
J. Phys. Chem. B
112
,
8241
(
2008
).
49.
P. J.
Molino
,
P. C.
Innis
,
M. J.
Higgins
,
R. M. I.
Kapsa
, and
G. G.
Wallace
,
Synth. Met.
200
,
40
(
2015
).
50.
A. J.
Bard
and
L. R.
Faulkner
,
Electrochem. Methods
2
,
580
(
2001
).
51.
X.
Xu
,
A.
Makaraviciute
,
J.
Pettersson
,
S.-L.
Zhang
,
L.
Nyholm
, and
Z.
Zhang
,
Sens. Actuators, B
283
,
146
(
2019
).
52.
A. R.
Harris
,
C.
Newbold
,
P.
Carter
,
R.
Cowan
, and
G. G.
Wallace
,
Front. Neurosci.
13
,
380
, (
2019
).
53.
A. R.
Harris
,
C.
Newbold
,
P.
Carter
,
R.
Cowan
, and
G. G.
Wallace
,
J. Neural Eng.
15
,
046015
(
2018
).
54.
A. R.
Harris
,
B. J.
Allitt
, and
A. G.
Paolini
,
Analyst
144
,
2973
(
2019
).
55.
A. R.
Harris
,
P.
Carter
,
R.
Cowan
, and
G. G.
Wallace
,
ChemElectroChem
8
, 1–14. (
2021
).
56.
N.
Laurens
,
P.
Koolwijk
, and
M. P. M.
De Maat
,
J. Thromb. Haemostasis
4
,
932
(
2006
).
57.
S.
Chattopadhyay
and
R. T.
Raines
,
Biopolymers
101
,
821
(
2014
).
58.
F.
Höök
,
M.
Rodahl
,
P.
Brzezinski
, and
B.
Kasemo
,
Langmuir
14
,
729
(
1998
).
59.
K.
Rechendorff
,
M. B.
Hovgaard
,
M.
Foss
,
V.
Zhdanov
, and
F.
Besenbacher
,
Langmuir
22
,
10885
(
2006
).
60.
P.
Roach
,
D.
Farrar
, and
C. C.
Perry
,
J. Am. Chem. Soc.
127
,
8168
(
2005
).
61.
M. S.
Lord
,
J. M.
Whitelock
,
A.
Simmons
,
R. L.
Williams
, and
B. K.
Milthorpe
,
Biointerphases
9
,
041002
(
2014
).
62.
M. C.-M.
Chung
,
Biochem. Educ.
12
,
146
(
1984
).
63.
J. A.
Wright
,
T.
Richards
, and
S. K.
Srai
,
Front. Pharmacol.
5
, 156 (2014).
65.
See supplementary material at http://dx.doi.org/10.1116/6.0000708 for mean raw QCM-D parameter shifts for the adsorption of transferrin, fibrinogen, and collagen to the PEDOT-XRU materials, and live/dead staining of human dermal fibroblasts to the various PEDOT-XRU polymers.

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