The development of 2D and 3D structures on the nanoscale containing viral nanoparticles (VNPs) as interesting nanobuilding blocks has come into focus for a bottom-up approach as an alternative to the top-down approach in nanobiotechnology. Our research has focused on the plant Tomato Bushy Stunt Virus (TBSV). In a previous study, we reported the impact of the pH value on the 2D assembly of viral monolayers. Here, we extend these studies into the third dimension by using specific interactions between the layers in combination with selective side chains on the viral capsid. The virus bilayer structure is prepared by an alternating deposition of His-tagged TBSV (4D6H-TBSV, first layer), Ni-NTA nanogold (second layer) complexes and 4D6H-TBSV, respectively, and 6D-TBSV (6xaspartic acid TBSV) as the third layer, i.e., the second layer of VNPs. The formed layer structures were imaged by using scanning force and scanning electron microscopy. The data show that a virus bilayer structure was successfully built up by means of the interaction between Ni-NTA nanogold and histidine. By comparing 4D6H- with 6D-TBSV in the third layer, the importance of these specific interactions is shown. This work paves the way for 3D nanodevices based on VNPs.
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July 2020
Research Article|
August 04 2020
Bottom-up assembly of a bilayer structure of icosahedral viral nanoparticles
C. Müller-Renno;
C. Müller-Renno
1
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin-Schrödinger-Str. 56, 67663 Kaiserslautern, Germany
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V. Rink;
V. Rink
1
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin-Schrödinger-Str. 56, 67663 Kaiserslautern, Germany
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M. Ani;
M. Ani
1
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin-Schrödinger-Str. 56, 67663 Kaiserslautern, Germany
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M. Braun;
M. Braun
2
RLP Agroscience GmbH
, Breitenweg 71, 67435 Neustadt/Weinstraße, Germany
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K. Boonrod;
K. Boonrod
2
RLP Agroscience GmbH
, Breitenweg 71, 67435 Neustadt/Weinstraße, Germany
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G. Krczal;
G. Krczal
2
RLP Agroscience GmbH
, Breitenweg 71, 67435 Neustadt/Weinstraße, Germany
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Ch. Ziegler
Ch. Ziegler
a)
1
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin-Schrödinger-Str. 56, 67663 Kaiserslautern, Germany
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a)
Electronic mail: [email protected]
Biointerphases 15, 041009 (2020)
Article history
Received:
April 20 2020
Accepted:
July 13 2020
Citation
C. Müller-Renno, V. Rink, M. Ani, M. Braun, K. Boonrod, G. Krczal, Ch. Ziegler; Bottom-up assembly of a bilayer structure of icosahedral viral nanoparticles. Biointerphases 1 July 2020; 15 (4): 041009. https://doi.org/10.1116/6.0000267
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