Copper (Cu) is an essential transition metal ion that acts as a cofactor in many key enzymes. Cu is also needed for several hallmarks of cancer, and many copper-binding proteins are upregulated in various cancers. However, Cu-dependent cellular mechanisms and molecular pathways involved in cancer progression are not known. Fundamental to a better understanding of such phenomena is the investigation of the Cu subcellular distribution in cancer cells. The authors here show that Time-of-Flight Secondary Ion Mass Spectrometry combined with delayed extraction can be successfully applied to probe Cu localization in fixed MDA-MB-231 breast cancer cells providing subcellular resolution. Interestingly, the authors find Cu to be accumulated at nuclear regions of the cancer cells.
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Copper distribution in breast cancer cells detected by time-of-flight secondary ion mass spectrometry with delayed extraction methodology
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December 2018
Research Article|
December 20 2018
Copper distribution in breast cancer cells detected by time-of-flight secondary ion mass spectrometry with delayed extraction methodology
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Stéphanie Blockhuys;
Stéphanie Blockhuys
1
Department of Biology and Biological Engineering, Chalmers University of Technology
, Kemigården 4, 412 96 Gothenburg, Sweden
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Per Malmberg
;
Per Malmberg
a)
2
Department of Chemistry and Chemical Engineering, Chalmers University of Technology
, Kemigården 4, 412 96 Gothenburg, Sweden
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Pernilla Wittung-Stafshede
Pernilla Wittung-Stafshede
a)
1
Department of Biology and Biological Engineering, Chalmers University of Technology
, Kemigården 4, 412 96 Gothenburg, Sweden
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Stéphanie Blockhuys
1
Per Malmberg
2,a)
Pernilla Wittung-Stafshede
1,a)
1
Department of Biology and Biological Engineering, Chalmers University of Technology
, Kemigården 4, 412 96 Gothenburg, Sweden
2
Department of Chemistry and Chemical Engineering, Chalmers University of Technology
, Kemigården 4, 412 96 Gothenburg, Sweden
a)
Electronic addresses: [email protected] and [email protected]
Biointerphases 13, 06E412 (2018)
Article history
Received:
August 26 2018
Accepted:
December 10 2018
Citation
Stéphanie Blockhuys, Per Malmberg, Pernilla Wittung-Stafshede; Copper distribution in breast cancer cells detected by time-of-flight secondary ion mass spectrometry with delayed extraction methodology. Biointerphases 1 December 2018; 13 (6): 06E412. https://doi.org/10.1116/1.5053814
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