The bacterial attachment to surfaces is the first step of biofilm formation. This attachment is governed by adhesion forces which act between the bacterium and the substrate. Such forces can be measured by single cell force spectroscopy, where a single bacterium is attached to a cantilever of a scanning force microscope, and force-distance curves are measured. For the productive sea-water bacterium Paracoccus seriniphilus, pH dependent measurements reveal the highest adhesion forces at pH 4. Adhesion forces measured at salinities between 0% and 4.5% NaCl are in general higher for higher salinity. However, there is an exception for 0.9% where a higher adhesion force was measured than expected. These results are in line with zeta potential measurements of the bacterium, which also show an exceptionally low zeta potential at 0.9% NaCl. In the absence of macromolecular interactions, the adhesion forces are thus governed by (unspecific) electrostatic interactions, which can be adjusted by pH and ionic strength. It is further shown that microstructures on the titanium surface increase the adhesion force. Growth medium reduces the interaction forces dramatically, most probably through macromolecular bridging.
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December 2017
Research Article|
November 06 2017
Adhesion forces of the sea-water bacterium Paracoccus seriniphilus on titanium: Influence of microstructures and environmental conditions
Neda Davoudi;
Neda Davoudi
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin Schrödinger-Straße 56, 67663 Kaiserslautern, Germany
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Katharina Huttenlochner;
Katharina Huttenlochner
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin Schrödinger-Straße 56, 67663 Kaiserslautern, Germany
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Jonas Chodorski;
Jonas Chodorski
Institute of Bioprocess Engineering, University of Kaiserslautern
, Gottlieb-Daimler-Straße 49, 67663 Kaiserslautern, Germany
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Christin Schlegel;
Christin Schlegel
Institute of Bioprocess Engineering, University of Kaiserslautern
, Gottlieb-Daimler-Straße 49, 67663 Kaiserslautern, Germany
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Martin Bohley;
Martin Bohley
Institute for Manufacturing Technology and Production Systems (FBK), University of Kaiserslautern
, Gottlieb-Daimler-Straße 42, 67663 Kaiserslautern, Germany
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Christine Müller-Renno;
Christine Müller-Renno
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin Schrödinger-Straße 56, 67663 Kaiserslautern, Germany
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Jan. C. Aurich;
Jan. C. Aurich
Institute for Manufacturing Technology and Production Systems (FBK), University of Kaiserslautern
, Gottlieb-Daimler-Straße 42, 67663 Kaiserslautern, Germany
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Roland Ulber;
Roland Ulber
Institute of Bioprocess Engineering, University of Kaiserslautern
, Gottlieb-Daimler-Straße 49, 67663 Kaiserslautern, Germany
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Christiane Ziegler
Christiane Ziegler
a)
Department of Physics and Research Center OPTIMAS, University of Kaiserslautern
, Erwin Schrödinger-Straße 56, 67663 Kaiserslautern, Germany
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a)
Electronic mail: cz@physik.uni-kl.de
Biointerphases 12, 05G606 (2017)
Article history
Received:
August 31 2017
Accepted:
October 20 2017
Citation
Neda Davoudi, Katharina Huttenlochner, Jonas Chodorski, Christin Schlegel, Martin Bohley, Christine Müller-Renno, Jan. C. Aurich, Roland Ulber, Christiane Ziegler; Adhesion forces of the sea-water bacterium Paracoccus seriniphilus on titanium: Influence of microstructures and environmental conditions. Biointerphases 1 December 2017; 12 (5): 05G606. https://doi.org/10.1116/1.5002676
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