Cytokines are small proteins that play fundamental roles in inflammatory processes in the human body. In particular, interleukin (IL)-6 is a multifunctional cytokine, whose increased levels are associated with infection, cancer, and inflammation. The quantification of IL-6 is therefore of primary importance in early stages of inflammation and in chronic diseases, but standard techniques are expensive, time-consuming, and usually rely on fluorescent or radioactive labels. Organic electronic devices and, in particular, organic field-effect transistors (OFETs) have been proposed in the recent years as novel platforms for label-free protein detection, exploiting as sensing unit surface-immobilized antibodies or aptamers. Here, the authors report two electrolyte-gated OFETs biosensors for IL-6 detection, featuring monoclonal antibodies and peptide aptamers adsorbed at the gate. Both strategies yield biosensors that can work on a wide range of IL-6 concentrations and exhibit a remarkable limit of detection of 1 pM. Eventually, electrolyte gated OFETs responses have been used to extract and compare the binding thermodynamics between the sensing moiety, immobilized at the gate electrode, and IL-6.
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Label-free detection of interleukin-6 using electrolyte gated organic field effect transistors
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December 2017
Research Article|
September 27 2017
Label-free detection of interleukin-6 using electrolyte gated organic field effect transistors
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Chiara Diacci;
Chiara Diacci
a)
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Marcello Berto;
Marcello Berto
b)
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Michele Di Lauro;
Michele Di Lauro
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Elena Bianchini;
Elena Bianchini
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Marcello Pinti;
Marcello Pinti
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Daniel T. Simon;
Daniel T. Simon
Laboratory of Organic Electronics, Department of Science and Technology, ITN, Linko¨ping University
, S-601 74 Norrko¨ping, Sweden
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Fabio Biscarini;
Fabio Biscarini
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Carlo A. Bortolotti
Carlo A. Bortolotti
c)
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
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Chiara Diacci
a)
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
Marcello Berto
b)
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
Michele Di Lauro
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
Elena Bianchini
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
Marcello Pinti
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
Daniel T. Simon
Laboratory of Organic Electronics, Department of Science and Technology, ITN, Linko¨ping University
, S-601 74 Norrko¨ping, Sweden
Fabio Biscarini
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
Carlo A. Bortolotti
c)
Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia
, via Campi 103, 41125 Modena, Italy
a)
Present address: Laboratory of Organic Electronics, Department of Science and Technology, ITN, Linko¨ping University, S-601 74 Norrko¨ping, Sweden.
b)
Present address: Dipartimento di Scienze Biomediche e Chirurgico Specialistiche, Università di Ferrara, Via Fossato di Mortara 17, 44121 Ferrara, Italy.
c)
Electronic mail: [email protected]
Biointerphases 12, 05F401 (2017)
Article history
Received:
July 26 2017
Accepted:
September 15 2017
Citation
Chiara Diacci, Marcello Berto, Michele Di Lauro, Elena Bianchini, Marcello Pinti, Daniel T. Simon, Fabio Biscarini, Carlo A. Bortolotti; Label-free detection of interleukin-6 using electrolyte gated organic field effect transistors. Biointerphases 1 December 2017; 12 (5): 05F401. https://doi.org/10.1116/1.4997760
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