Unmodified gelatin (uG) is widely used as a coating material in cell culture for improving surface properties. In this study, the authors investigated if gelatin methacrylamide (GM) with a medium degree of methacrylamide modification (GM1.5) and a high degree of methacrylamide modification (GM4) are equally suitable for this purpose. Therefore, gold surfaces were coated with uG, GM1.5, and GM4 by adsorption of the polymers on the surfaces. Coating success was confirmed by spectroscopic ellipsometry, contact angle measurements, surface plasmon resonance spectroscopy (SPRS), and atomic force microscopy (AFM). The authors found that upon adsorption of uG, GM1.5, a nd GM4 on gold, thin films with thicknesses of 2.95 nm, 2.50 nm, and 2.26 nm were formed. The coated surfaces showed advancing contact angles of 46° (uG and GM1.5) and 52° (GM4) without alteration of the surface roughness determined by AFM. Protein adsorption taking place on the coated surfaces was measured during contact of the surfaces with fetal calf serum by SPRS. Protein adsorption on the coated surfaces was reduced by the factor of 6.4 (uG), 5.4 (GM1.5), and 4.6 (GM4) compared to gold surfaces. Human fibroblasts cultured on the surfaces showed excellent viability shown by water soluble tetrazolium salt assay as well as live/dead staining with propidium iodide and fluorescein diacetate. No cytotoxic effects of the GM coated surfaces were observed, giving rise to the conclusion that GMs are suitable materials as coatings in cell culture.
Skip Nav Destination
Article navigation
June 2016
Research Article|
May 13 2016
Gelatin methacrylamide as coating material in cell culture
Michael Egger;
Michael Egger
Institute of Interfacial Process Engineering and Plasma Technology IGVP,
University of Stuttgart
, Nobelstr. 12, 70569 Stuttgart, Germany
Search for other works by this author on:
Günter E. M. Tovar;
Günter E. M. Tovar
a)
Institute of Interfacial Process Engineering and Plasma Technology IGVP,
University of Stuttgart
, Nobelstr. 12, 70569 Stuttgart, Germany
and Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB
, Nobelstr. 12, 70569 Stuttgart, Germany
Search for other works by this author on:
Eva Hoch;
Eva Hoch
Institute of Interfacial Process Engineering and Plasma Technology IGVP,
University of Stuttgart
, Nobelstr. 12, 70569 Stuttgart, Germany
Search for other works by this author on:
Alexander Southan
Alexander Southan
b)
Institute of Interfacial Process Engineering and Plasma Technology IGVP,
University of Stuttgart
, Nobelstr. 12, 70569 Stuttgart, Germany
Search for other works by this author on:
a)
Electronic mail: Guenter.Tovar@igb.fraunhofer.de
b)
Electronic mail: alexander.southan@igvp.uni-stuttgart.de
Biointerphases 11, 021007 (2016)
Article history
Received:
February 01 2016
Accepted:
May 03 2016
Citation
Michael Egger, Günter E. M. Tovar, Eva Hoch, Alexander Southan; Gelatin methacrylamide as coating material in cell culture. Biointerphases 1 June 2016; 11 (2): 021007. https://doi.org/10.1116/1.4949545
Download citation file:
Sign in
Don't already have an account? Register
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Sign in via your Institution
Sign in via your InstitutionPay-Per-View Access
$40.00
Citing articles via
Related Content
Poly(vinylidene fluoride-co-hexafluoropropylene)-graft-poly(dopamine methacrylamide) copolymers: A nonlinear dielectric material for high energy density storage
Appl. Phys. Lett. (December 2013)
Regulating the uptake of poly(N-(2-hydroxypropyl) methacrylamide)-based micelles in cells cultured on micropatterned surfaces
Biointerphases (July 2021)
Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel
Biointerphases (March 2012)
Non-steroidal anti-inflamatory drugs as guest of dendronized polymeric nanocomposites
AIP Conference Proceedings (May 2016)
Enhancement of the first normal stress coefficient and dynamic moduli during shear thickening of a polymer solution
Journal of Rheology (July 2003)