Plain and microstructured cp-titanium samples were studied as possible biofilm reactor substrates. The biofilms were grown by exposition of the titanium samples to bacteria in a flow cell. As bacteria the rod shaped gram negative Pseudomonas fluorescens and the spherical gram negative Paracoccus seriniphilus were chosen. Afterward, the samples were cleaned in subsequent steps: First, with a standard solvent based cleaning procedure with acetone, isopropanol, and ultrapure water and second by oxygen plasma sputtering. It will be demonstrated by means of x-ray photoelectron spectroscopy, fluorescence microscopy, and confocal laser scanning microscopy that oxygen plasma cleaning is a necessary and reliant tool to fully clean and restore titanium surfaces contaminated with a biofilm. The microstructured surfaces act beneficial to biofilm growth, while still being fully restorable after biofilm contamination. Scanning electron microscopy images additionally show, that the plasma process does not affect the microstructures. The presented data show the importance of the cleaning procedure. Just using solvents does not remove the biofilm and all its components reliably while a cleaning process by oxygen plasma regenerates the surfaces.
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March 2015
Research Article|
February 10 2015
Cleaning of titanium substrates after application in a bioreactor
Mathias Fingerle;
Mathias Fingerle
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Oliver Köhler;
Oliver Köhler
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Christina Rösch;
Christina Rösch
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Fabian Kratz;
Fabian Kratz
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Christian Scheibe;
Christian Scheibe
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Neda Davoudi;
Neda Davoudi
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Christine Müller-Renno;
Christine Müller-Renno
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Christiane Ziegler;
Christiane Ziegler
a)
Department of Physics and Research Centre OPTIMAS,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Manuel Huster;
Manuel Huster
Institute of Bioprocess Engineering,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Christin Schlegel;
Christin Schlegel
Institute of Bioprocess Engineering,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Roland Ulber;
Roland Ulber
Institute of Bioprocess Engineering,
University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Martin Bohley;
Martin Bohley
Institute for Manufacturing Technology and Production Systems
, University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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Jan C. Aurich
Jan C. Aurich
Institute for Manufacturing Technology and Production Systems
, University of Kaiserslautern
, D-67663 Kaiserslautern, Germany
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a)
Electronic mail: [email protected]
Biointerphases 10, 019007 (2015)
Article history
Received:
December 15 2014
Accepted:
January 28 2015
Citation
Mathias Fingerle, Oliver Köhler, Christina Rösch, Fabian Kratz, Christian Scheibe, Neda Davoudi, Christine Müller-Renno, Christiane Ziegler, Manuel Huster, Christin Schlegel, Roland Ulber, Martin Bohley, Jan C. Aurich; Cleaning of titanium substrates after application in a bioreactor. Biointerphases 1 March 2015; 10 (1): 019007. https://doi.org/10.1116/1.4907754
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