The measurement of force–distance curves on a single bacterium provides a unique opportunity to detect properties such as the turgor pressure under various environmental conditions. Marine bacteria are very interesting candidates for the production of pharmaceuticals, but are only little studied so far. Therefore, the elastic behavior of Paracoccus seriniphilus, an enzyme producing marine organism, is presented in this study. After a careful evaluation of the optimal measurement conditions, the spring constant and the turgor pressure are determined as a function of ionic strength and pH. Whereas the ionic strength changes the turgor pressure passively, the results give a hint that the change to acidic pH increases the turgor pressure by an active mechanism. Furthermore, it could be shown, that P. seriniphilus has adhesive protrusions outside its cell wall.
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March 2015
Research Article|
February 02 2015
Nanomechanical properties of the sea-water bacterium Paracoccus seriniphilus—A scanning force microscopy approach
Neda Davoudi;
Neda Davoudi
Department of Physics and Research Center OPTIMAS,
University of Kaiserslautern
, Erwin Schrödinger Strasse 56, 67663 Kaiserslautern, Germany
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Christine Müller-Renno;
Christine Müller-Renno
Department of Physics and Research Center OPTIMAS,
University of Kaiserslautern
, Erwin Schrödinger Strasse 56, 67663 Kaiserslautern, Germany
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Christiane Ziegler;
Christiane Ziegler
a)
Department of Physics and Research Center OPTIMAS,
University of Kaiserslautern
, Erwin Schrödinger Strasse 56, 67663 Kaiserslautern, Germany
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Indek Raid;
Indek Raid
Institute for Measurement and Sensor Technology,
University of Kaiserslautern
, Gottlieb-Daimler-Strasse 46, 67663 Kaiserslautern, Germany
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Jörg Seewig;
Jörg Seewig
Institute for Measurement and Sensor Technology,
University of Kaiserslautern
, Gottlieb-Daimler-Strasse 46, 67663 Kaiserslautern, Germany
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Christin Schlegel;
Christin Schlegel
Institute of Bioprocess Engineering,
University of Kaiserslautern
, Gottlieb-Daimler-Strasse 46, 67663 Kaiserslautern, Germany
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Kai Muffler;
Kai Muffler
Institute of Bioprocess Engineering,
University of Kaiserslautern
, Gottlieb-Daimler-Strasse 46, 67663 Kaiserslautern, Germany
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Roland Ulber
Roland Ulber
Institute of Bioprocess Engineering,
University of Kaiserslautern
, Gottlieb-Daimler-Strasse 46, 67663 Kaiserslautern, Germany
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a)
Electronic mail: cz@physik.uni-kl.de
Biointerphases 10, 019004 (2015)
Article history
Received:
December 12 2014
Accepted:
January 16 2015
Citation
Neda Davoudi, Christine Müller-Renno, Christiane Ziegler, Indek Raid, Jörg Seewig, Christin Schlegel, Kai Muffler, Roland Ulber; Nanomechanical properties of the sea-water bacterium Paracoccus seriniphilus—A scanning force microscopy approach. Biointerphases 1 March 2015; 10 (1): 019004. https://doi.org/10.1116/1.4906862
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