Estimating the density of organism living in deep sea scatting layers is of key importance for understanding the biomass in the mesopelagic layers. Scientific echosounders are routinely used for this task; however, new imaging sonar technologies pose the opportunity for estimating density of organism, as well as identification at smaller scales. During the 2013 NOAA KONA Integrated Ecosystem, a Dual Frequency Identification Sonar (DIDSON) (SoundMetrics Inc.) was used to estimate the density, length of organisms in the deep sea scattering layers during nighttime and daytime along the KONA coast of the island of Hawaii. At each station, an EK60 38 kHz echosounder was used to find the depth of the deep sea scattering layers, and the DIDSON was lower into the layer (or layers if two were present) (about 500 and 600 m), and underneath the deeper layer (about 800 m). A total of 4621 organisms were counted and sized. We estimated densities ranging from 6 to 1 organism/m3. Density shows some variation between locations and depth and organism as big as 3 m were sighted.