Focused ultrasound treatments of the spinal cord may be facilitated using a phased array transducer and beamforming to correct spine-induced focal aberrations. Simulations can non-invasively calculate aberration corrections using x-ray computed tomography (CT) data that are correlated to density (ρ) and longitudinal sound speed (cL). We aimed to optimize vertebral lamina-specific functions at a physiological temperature (37 °C) to maximize time domain simulation accuracy. Odd-numbered ex vivo human thoracic vertebrae were imaged with a clinical CT-scanner (0.511 × 0.511 × 0.5 mm), then sonicated with a transducer (514 kHz) focused on the canal via the vertebral lamina. Vertebra-induced signal time shifts were extracted from pressure waveforms recorded within the canals. Measurements were repeated 5× per vertebra, with 2.5 mm vertical vertebra shifts between measurements. Linear functions relating cL with CT-derived density were optimized. The optimized function was m/s, where w denotes water, giving the tested laminae a mean bulk density of 1600 ± 30 kg/m3 and a mean bulk cL of 1670 ± 60 m/s. The optimized lamina function was accurate to when implemented in a multi-layered ray acoustics model. This modelling accuracy will improve trans-spine ultrasound beamforming.
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