The effects of click rate on the auditory brainstem response of bottlenose dolphins

Rate manipulations can be used to study adaptation processes in the auditory nerve and brainstem. For this reason, rate effects on the click-evoked auditory brainstem response (ABR) have been investigated in many mammals, including humans. In this study, click-evoked ABRs were obtained in eight bottlenose dolphins (Tursiops truncatus) while varying stimulus rate using both conventional averaging and maximum length sequences (MLSs), which allow disentangling ABRs that overlap in time and thus permit the study of adaptation at high rates. Dolphins varied in age and upper cutoff frequency of hearing. Conventional stimulation rates were 25, 50, and 100 Hz and average MLS rates were approximately 50, 100, 250, 500, 1000, 2500, and 5000 Hz. Click peak-equivalent sound pressure levels for all conditions were 135 dB re 1 μPa. ABRs were observed in all dolphins, at all stimulus rates. With increasing rate, peak latencies increased and peak amplitudes decreased. There was a trend for an increase in the interwave intervals with increasing rate, which appeared more robust for the dolphins with a full range of hearing. For those rates where ABRs were obtained for both conventional and MLS approaches, the latencies of the mean data were in good agreement.