In this study, the speed of sound (SOS) of two types of rat livers (eight normal livers, four cirrhotic livers) was measured with a scanning acoustic microscope using two transducers, one of which had an 80-MHz and the other a 250-MHz center frequency. The 250-MHz transducer had a better spatial resolution adapted to studying fiber or hepatic parenchymal cells. In normal livers, averages of the SOS values were from 1598 to 1677 m/s at 80-MHz and from 1568 to 1668 m/s at 250-MHz. In the fiber tissue of cirrhotic livers, averages of the SOS values were from 1645 to 1658 m/s at 80-MHz and from 1610 to 1695 m/s at 250-MHz, while the SOS values in the other tissue of cirrhotic livers ranged from 1644 to 1709 m/s at 80-MHz and from 1641 to 1715 m/s at 250-MHz. In one liver, SOS in fiber tissue was larger than that of tissues without fiber while in others it was lower. The resulting two-dimensional SOS maps provide a unique quantitative insight of liver acoustic microstructures in a healthy liver and in a cirrhotic ones. This study would be helpful to understand the complex relationship between acoustic properties and liver disease including fiber tissue.

1.
Agemura
,
D. H.
,
O'Brien
,
W. D.
,
Olerud
,
J. E.
,
Chun
,
L. E.
, and
Eyre
,
D. E.
(
1990
). “
Ultrasonic propagation properties of articular cartilage at 100 MHz
,”
J. Acoust. Soc. Am.
87
,
1786
1791
.
2.
Akashi
,
N.
,
Kushibiki
,
J.
, and
Dunn
,
F.
(
2003
). “
An analytic method of frequency dependence of acoustic properties of biological media in the VHF/UHF range using the bioultrasonic spectroscopy system
,”
Technical report of IEICE, US2003-52
(in Japanese).
3.
Bamber
,
J.
, and
Hill
,
C.
(
1979
). “
Ultrasonic attenuation and propagation speed in mammalian as a function of temperature
,”
Ultrasound Med. Biol.
5
,
149
157
.
4.
Bamber
,
J.
, and
Hill
,
C.
(
1981
). “
Acoustic properties of normal and cancerous human liver
,”
Ultrasound Med. Biol.
7
,
121
133
.
5.
Behnam
,
H.
,
Ohtsuki
,
S.
, and
Tanaka
,
M.
(
1998
). “
Phase correction on ultrasound speed measurement
,”
J. Acoust. Soc. Jpn.
19
(
2
),
141
150
.
6.
Brewin
,
M.
,
Srodon
,
P.
,
Greenwald
,
S.
, and
Birch
,
M.
(
2014
). “
Carotid atherosclerotic plaque characterization by measurement of ultrasound sound speed in vitro at high frequency, 20 MHz
,”
Ultrasonics
54
,
428
441
.
7.
Chen
,
X.
,
Shen
,
Y.
,
Zheng
,
Y.
,
Lin
,
H.
,
Guo
,
Y.
,
Zhu
,
Y.
,
Zhang
,
X.
,
Wang
,
T.
, and
Chen
,
S.
(
2013
). “
Quantification of liver viscoelasticity with acoustic radiation force: A study of hepatic fibrosis in a rat model
,”
Ultrasound Med. Biol.
39
,
2091
2102
.
8.
Choi
,
M.
,
Guntur
,
S.
,
Lee
,
J.
,
Paeng
,
D.
,
Lee
,
K.
, and
Coleman
,
A.
(
2011
). “
Changes in ultrasonic propagation of liver tissue in vitro during heating- cooling cycle concomitant with thermal coagulation
,”
Ultrasound Med. Biol.
37
,
2000
2012
.
9.
Choi
,
W.
,
Kim
,
H.
,
Cha
,
J.
,
Shin
,
H.
,
Kim
,
H.
,
Chae
,
E.
, and
Hong
,
M.
(
2014
). “
Predicting prognostic factors of breast cancer using share wave elastography
,”
Ultrasound Med. Biol.
40
,
269
274
.
10.
Gawne-Cain
,
M.
,
Smith
,
E.
,
Darby
,
M.
, and
Wilson
,
G.
(
1995
). “
The use of ultrasound for monitoring breast tumor response to pro-adjuvant therapy
,”
Clin. Radiol.
50
,
681
686
.
11.
Ghoshal
,
G.
,
Lavarello
,
R.
,
Kemmerer
,
J.
,
Miller
,
R.
, and
Oelze
,
M.
(
2012
). “
Ex vivo study of quantitative ultrasound parameters in fatty rabbit livers
,”
Ultrasound Med. Biol.
38
,
2238
2248
.
12.
Goss
,
S.
,
Johnston
,
R.
, and
Dunn
,
F.
(
1978
). “
Comprehensive compilation of empirical ultrasonic properties of mammalian tissue
,”
J. Acoust. Soc. Am.
64
,
432
457
.
13.
Greenspan
,
M.
, and
Tschegg
,
C. E.
(
1959
). “
Tables of the speed of sound in water
,”
J. Acoust. Soc. Am.
31
,
75
76
.
14.
Harada
,
N.
,
Shirabe
,
K.
,
Ijichi
,
H.
,
Matono
,
R.
,
Uchiyama
,
H.
,
Yoshizumi
,
T.
,
Taketomi
,
A.
,
Soejima
,
Y.
, and
Maehara
,
Y.
(
2012
). “
Acoustic radiation force impulse imaging predicts postoperative ascites resulting from curative hepatic section for hepatocellular carcinoma
,”
Surgery
151
,
837
843
.
15.
Hill
,
C.
,
Bamber
,
J.
, and
Haar
,
G.
(
2005
).
Physical Principles of Medical Ultrasonics
, 2nd ed. (
John Wiley & Sons, Ltd.
,
New York
), Chap. 5, pp.
167
190
.
16.
Jakob
,
A.
,
Bendr
,
M.
,
Knoll
,
T.
, and
Lemor
,
R.
(
2009
). “
Comparison of different piezoelectric materials for GHz acoustic microscopy
,” in
IEEE International Ultrasonics Symposium Proceedings
, pp.
1722
1725
.
17.
Kobayashi
,
K.
,
Yoshida
,
S.
,
Saijo
,
Y.
, and
Hozumi
,
N.
(
2014
). “
Acoustic impedance microscopy for biological tissue characterization
,”
Ultrasonics
54
,
1922
1928
.
18.
Lin
,
T.
,
Ophir
,
J.
, and
Potter
,
G.
(
1988
). “
Correlation of ultrasonic attenuation with pathologic fat and fibrosis in liver disease
,”
Ultrasound Med. Biol.
14
,
729
734
.
19.
Lizzi
,
F. L.
,
Greenebaum
,
M.
,
Feleppa
,
E. J.
,
Elbaum
,
M.
, and
Coleman
,
D. J.
(
1983
). “
Theoretical framework for spectrum analysis in ultrasonic tissue characterization
,”
J. Acoust. Soc. Am.
73
,
1366
1373
.
20.
Meziri
,
M.
,
Pereira
,
W.
,
Abdelwahab
,
A.
,
Degott
,
C.
, and
Laugier
,
P.
(
2005
). “
In vitro chronic hepatic disease characterization with a multiparametric ultrasonic approach
,”
Ultrasonics
43
,
305
313
.
21.
Nakajima
,
S.
,
Shibuya
,
K.
,
Kamiyama
,
N.
, and
Sumino
,
K.
(
2010
). “
Comparison of ultrasonic colored image views produced by application of statistical analysis of radio-frequency signals and histological findings in patients with chronic hepatitis C
,”
J. Med. Ultrason.
37
,
51
58
.
22.
Nakano
,
H.
,
Naito
,
K.
,
Suzuki
,
S.
,
Naito
,
K.
,
Kobayashi
,
K.
, and
Yamamoto
,
S.
(
2015
). “
Scanning acoustic microscopy imaging of tongue squamous cell carcinomas discriminates speed-of-sound between lesions and healthy regions in the mucous epithelium
,”
J. Oral Maxillofacial Surg., Med., Pathol.
27
,
16
19
.
23.
Nam
,
K.
,
Rosado-Mendez
,
I. M.
,
Wirtzfeld
,
L. A.
,
Kumar
,
V.
,
Madsen
,
E. L.
,
Ghoshal
,
G.
,
Pawlicki
,
A. D.
,
Oelze
,
M. L.
,
Lavarello
,
R. J.
,
Bigelow
,
T. A.
,
Zagzebski
,
J. A.
,
O'Brien
,
W. D.
, and
Hall
,
T. J.
(
2012
). “
Cross imaging system comparison of backscatter coefficient estimates from a tissue-mimicking material
,”
J. Acoust. Soc. Am.
132
,
1319
1324
.
24.
Ozkan
,
F.
,
Yavuz
,
Y.
,
Inci
,
M.
,
Altunoluk
,
B.
,
Ozcan
,
N.
,
Yuksel
,
M.
,
Sayarkioglu
,
H.
, and
Dogan
,
E.
(
2013
). “
Interobserver variability of ultrasound elastography in transplant kidneys: Correlations with clinical-Doppler parameters
,”
Ultrasound Med. Biol.
39
,
4
9
.
25.
Paolo
,
R.
,
Chiara
,
M.
,
Vito
,
C.
,
Andrea
,
P.
,
Andrea
,
M.
,
Pietro
,
L.
,
Ugo
,
D.
,
Giancarlo
,
L.
,
Elena
,
M.
,
Ester
,
M.
,
Giovanna
,
P.
,
Mattia
,
S.
,
Caterina
,
F.
,
Raffaele
,
M.
, and
Gloria
,
T.
(
2013
). “
Ultrasound evaluation of liver fibrosis: Preliminary experience with acoustic structure quantification (ASQ) software
,”
La radiologia medica
118
,
995
1010
.
26.
Ping
,
Z.
,
Shuang-Ming
,
T.
,
Ying
,
Q.
,
Jin
,
D.
, and
Lu
,
Z.
(
2013
). “
Application of acoustic radiation force impulse imaging for the evaluation of focal liver lesion elasticity
,”
Hepatobiliary Pancreatic Dis. Int.
12
,
165
170
.
27.
Saijo
,
Y.
,
Hozumi
,
N.
,
Lee
,
C.
,
Nagao
,
M.
,
Kobayashi
,
K.
,
Oakada
,
N.
,
Tanaka
,
N.
,
Filho
,
E.
,
Sasai
,
H.
,
Tanaka
,
M.
, and
Yambe
,
T.
(
2006
). “
Ultrasonic speed microscopy for imaging of coronary artery
,”
Ultrasonics
44
,
E51
E55
.
28.
Sasaki
,
H.
,
Saijo
,
Y.
,
Tanaka
,
M.
, and
Nitta
,
S.
(
2003
). “
Influence of tissue preparation on the acoustic properties of tissue sections at high frequencies
,”
Ultrasound Med. Biol.
29
,
1367
1372
.
29.
Sasaki
,
H.
,
Saijo
,
Y.
,
Tanaka
,
M.
,
Okawai
,
H.
,
Terasawa
,
Y.
,
Yambe
,
T.
, and
Nitta
,
S.
(
1996
). “
Influence of tissue preparation on the high-frequency acoustic properties of normal kidney tissue
,”
Ultrasound Med. Biol.
22
,
1261
1265
.
30.
Shigemoto
,
H.
,
Sugimoto
,
T.
,
Hachiya
,
H.
,
Ebara
,
M.
,
Fukuda
,
H.
,
Kato
,
K.
, and
Ando
,
H.
(
2001
). “
Measurement of acoustic property distributions of diseased heart and liver tissues
,”
Jpn. J. Appl. Phys.
40
,
3907
3911
.
31.
Sporea
,
I.
,
Bota
,
S.
,
Peck-Radosavljevic
,
M.
,
Sirli
,
R.
,
Tanaka
,
H.
,
Iijima
,
H.
,
Badea
,
R.
,
Lupsor
,
M.
,
Fierbinteanu-Braticevici
,
C.
,
Petrisor
,
A.
,
Saito
,
H.
,
Ebinuma
,
H.
,
Friedrich-Rust
,
M.
,
Sarrazin
,
C.
,
Takahashi
,
H.
,
Ono
,
N.
,
Piscaglia
,
F.
,
Borghi
,
A.
,
D'Onofrio
,
M.
,
Gallotti
,
A.
,
Ferlitsch
,
A.
,
Popescu
,
A.
, and
Danila
,
M.
(
2012
). “
Acoustic radiation force impulse elastography for fibrosis evaluation in patients with chronic hepatitis C
,”
Eur. J. Radiol.
81
,
4112
4118
.
32.
Tanaka
,
N.
,
Kobayashi
,
K.
,
Hozumi
,
N.
,
Saijo
,
Y.
,
Tanaka
,
M.
, and
Ohtsuki
,
S.
(
2005
). “
Estimation of echo component in sound speed microscopy for biological tissue
,”
The Institute of Electronics, Information and Communication Engineers Technical Report
, Japan, Vol. 105, pp.
21
26
.
33.
Tanaka
,
Y.
,
Saijo
,
Y.
,
Fujihara
,
Y.
,
Nishizawa
,
S.
,
Nagata
,
S.
,
Ogasawara
,
T.
,
Asawa
,
Y.
,
Takato
,
T.
, and
Hoshi
,
K.
(
2012
). “
Evaluation of the implant type tissue-engineered cartilage by scanning acoustic microscopy
,”
J. Biosci. Bioeng.
113
,
252
257
.
34.
Techavipoo
,
U.
,
Varghese
,
T.
,
Chen
,
Q.
,
Stiles
,
T.
, and
Frank
,
G.
(
2004
). “
Temperature dependence of ultrasonic propagation speed and attenuation in excised canine lover tissue measured using transmitted and reflected pulses
,”
J. Acoust. Soc. Am.
115
,
2859
2865
.
35.
Towa
,
R.
,
Miller
,
R.
,
Frizzell
,
L.
,
Zachary
,
J.
, and
O'Brien
,
W.
(
2002
). “
Attenuation coefficient and propagation speed estimates of rat and pig intercostal tissue as a function of temperature
,”
IEEE Trans. Ultrasonics, Ferroelect. Freq. Control
49
,
1411
1420
.
36.
Ye
,
S.
,
Harasiewicz
,
K.
,
Pavlin
,
C.
, and
Foster
,
F.
(
1995
). “
Ultrasound characterization of normal ocular tissue in the frequency range from 50 MHz to 100 MHz
,”
IEEE Trans. Ultrason. Ferroelectr., Freq. Control
42
,
8
14
.
37.
Zhao
,
X.
,
Akhtar
,
R.
,
Nijenhuis
,
N.
,
Wilkinson
,
S.
,
Murphy
,
L.
,
Ballestrem
,
C.
,
Sherratt
,
M.
,
Watson
,
R.
, and
Derby
,
B.
(
2012
). “
Multi-layer phase analysis: Quantifying the elastic properties of soft tissues and live cells with ultra-high-frequency scanning acoustic microscopy
,”
IEEE Trans. Ultrason. Ferroelectr., Freq. Control
59
,
610
620
.
38.
Zhi
,
H.
,
Xiao
,
X.
,
Ou
,
B.
,
Zhong
,
W.
,
Zhao
,
Z.
,
Zhao
,
X.
,
Yang
,
H.
, and
Luo
,
B.
(
2012
). “
Could ultrasonic elastography help the diagnosis of small (<2 cm) breast cancer with the usage of sonographic BI-RADS classification?
,”
Eur. J. Radiol.
81
,
3216
3221
.
39.
Zocco
,
M.
,
Garcovich
,
M.
,
Lupascu
,
A.
,
Stasio
,
E.
,
Roccarina
,
D.
,
Annicchiarico
,
B.
,
Riccardi
,
L.
,
Ainora
,
M.
,
Ponziani
,
F.
,
Caracciolo
,
G.
,
Rapaccini
,
G.
,
Landolfi
,
R.
,
Siciliano
,
M.
,
Pompili
,
M.
, and
Gasbarrini
,
A.
(
2013
). “
Early prediction response to sorafenib in patients with advanced hepatocellular carcinoma: The role of dynamic contrast enhanced ultrasound
,”
J. Hepatology
59
,
1014
1021
.
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