Cultured human cervical cancer (HeLa) and rat mammary carcinoma (R3230Ac) cells were transfected with vectors encoding green fluorescent protein (GFP) under the control of hsp70B promoter. Aliquots of transfected cells were placed in thin-wall polymerase chain reaction tubes and exposed to high intensity focused ultrasound (HIFU) at a peak negative pressure . By adjusting the duty cycle of the HIFU transducer, the cell suspensions were heated to a peak temperature from in . Exposure dependent cell viability and gene activation were evaluated. For a HIFU exposure, cell viability dropped from 95% at to 13% at . Concomitantly, gene activation in sublethally injured tumor cells increased from 4% at to 41% at . A similar trend was observed at peak temperature as the exposure time increased from . Further increase of exposure duration to led to significantly reduced cell viability and lower overall gene activation in exposed cells. Altogether, maximum HIFU-induced gene activation was achieved at in . Under these experimental conditions, HIFU-induced gene activation was found to be produced primarily by thermal rather than mechanical stresses.
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November 2005
November 01 2005
High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro
Yunbo Liu;
Yunbo Liu
Department of Mechanical Engineering and Materials Science,
Duke University
, Durham, North Carolina 27708
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Takashi Kon;
Takashi Kon
Department of Radiation Oncology,
Duke University Medical Center
, Durham, North Carolina 27708
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Chuanyuan Li;
Chuanyuan Li
Department of Radiation Oncology,
Duke University Medical Center
, Durham, North Carolina 27708
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a)
Author to whom correspondence should be addressed; electronic mail: pzhong@duke.edu
J. Acoust. Soc. Am. 118, 3328–3336 (2005)
Article history
Received:
May 18 2005
Accepted:
July 28 2005
Citation
Yunbo Liu, Takashi Kon, Chuanyuan Li, Pei Zhong; High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro. J. Acoust. Soc. Am. 1 November 2005; 118 (5): 3328–3336. https://doi.org/10.1121/1.2041247
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