Kainic acid (KA) is a potent glutamate analog that can temporarily or permanently damage glutamatergic neurons. The purpose of the present study was to determine the short- and long-term effects of KA on chicken otoacoustic emissions and cochlear potentials. A chronic electrode was used to record the compound action potential (CAP), cochlear microphonic (CM), and the slow, positive neural potential (SPNP), a predominantly dc response. The CM, CAP, SPNP, and distortion product otoacoustic emissions (DPOAEs) were recorded before and after infusing 10 μl of a low dose (KA-L, 0.3 mM) or high dose (KA-H, 5 mM) of KA into scala tympani. KA caused a rapid and large reduction in CAP and SPNP amplitude in both the KA-H and KA-L groups; however, the CM and DPOAEs were largely unchanged. The amplitude of the CAP and SPNP in the KA-L group began to recover around 1 week post-KA, but was approximately 50% below normal at 4 weeks post-KA. In contrast, the CAP and SPNP showed no signs of recovery in the KA-H group. The results suggest that KA has no effect on the CM and DPOAEs generated by the hair cells, but selectively damages the CAP generated by the cochlear ganglion neurons. The reduction in the avian SPNP suggests that the response originates in the cochlear afferent neurons, unlike the summating potential (SP) in mammals that is generated in hair cells.
REFERENCES
1.
Adler
, H. J.
, Poje
, C. P.
, and Saunders
, J. C.
(1993
). “Recovery of auditory function and structure in the chick after two intense pure-tone exposures
,” Hearing Res.
71
, 214
–224
. 2.
Bledsoe
, Jr., S. C.
, Bobbin
, R. P.
, and Chihal
, D. M.
(1981
). “Kainic acid: An evaluation of its action on cochlear potentials
,” Hearing Res.
4
, 109
–120
. 3.
Bohne, B. (1976). “Mechanisms of noise damage in the inner ear,” in Effects of Noise on Hearing, edited by D. Henderson, R. P. Hamernik, D. S. Dosanjh, and J. H. Mills (Raven, New York), pp. 41–68.
4.
Burgoyne
, R. D.
, Graham
, M. E.
, and Cambray-Deakin
, M.
(1993
). “Neurotrophic effects of NMDA receptor activation on developing cerebellar granule cells
,” J. Neurocytol.
22
, 689
–695
. 5.
Chen
, C. K.
, Silverstein
, F. S.
, Fisher
, S. K.
, Statman
, D.
, and Johnston
, M. V.
(1988
). “Perinatal hypoxic-ischemic brain injury enhances quisqualic acid-stimulated phosphoinositide turnover
,” J. Neurochem.
51
, 353
–359
. 6.
Chen
, L.
, Salvi
, R. J.
, and Hashino
, E.
(1993
). “Recovery of CAP threshold and amplitude in chickens following kanamycin ototoxicity
,” Hearing Res.
69
, 15
–24
. 7.
Choi
, D. W.
, and Rothman
, S. M.
(1990
). “The role of glutamate neurotoxicity in hypoxic-ischemic neuronal death
,” Annu. Rev. Neurosci.
13
, 171
–182
. 8.
Cline
, H. T.
(1991
). “Activity-dependent plasticity in the visual systems of frogs and fish
,” Trends Neurosci.
14
, 104
–111
. 9.
Corwin
, J. T.
, and Cotanche
, D. A.
(1988
). “Regeneration of sensory hair cells after acoustic trauma
,” Science
240
, 1772
–1774
. 10.
d’Aldin
, C. G.
, Ruel
, J.
, Assie
, R.
, Pujol
, R.
, and Puel
, J. L.
(1997
). “Implication of NMDA type glutamate receptors in neural regeneration and neoformation of synapses after excitotoxic injury in the guinea pig cochlea
,” Int. J. Dev. Neurosci.
15
, 619
–629
. 11.
Dolan
, D. F.
, Nuttall
, A. L.
, and Avinash
, G.
(1990
). “Asynchronous neural activity recorded from the round window
,” J. Acoust. Soc. Am.
87
, 2621
–2627
. 12.
Duckert
, L. G.
, and Rubel
, E. W.
(1990
). “Ultrastructural observations on regenerating hair cells in the chick basilar papilla
,” Hearing Res.
48
, 161
–182
. 13.
Duckert
, L. G.
, and Rubel
, E. W.
(1993
). “Current concepts in hair cell regeneration
,” Otolaryngol. Clin. North Am.
26
, 873
–901
. 14.
Durrant
, J. D.
, Wang
, J.
, Ding
, D. L.
, and Salvi
, R. J.
(1998
). “Are inner or outer hair cells the source of summating potentials recorded from the round window?
,” J. Acoust. Soc. Am.
104
, 370
–377
. 15.
Ernfors
, P.
, Van De Water
, T.
, Loring
, J.
, and Jaenisch
, R.
(1995
). “Complementary roles of BDNF and NT-3 in vestibular and auditory development
,” Neuron
14
, 1153
–1164
. 16.
Froymovich
, O.
, Rebala
, V.
, Salvi
, R. J.
, and Rassael
, H.
(1995
). “Long-term effect of acoustic trauma on distortion product otoacoustic emissions in chickens
,” J. Acoust. Soc. Am.
97
, 3021
–3029
. 17.
Gil-Loyzaga
, P.
, and Pujol
, R.
(1990
). “Neurotoxicity of kainic acid in the rat cochlea during early developmental stages
,” Eur. Arch. Oto-Rhino-Laryngol.
248
, 40
–48
. 18.
Hori
, N.
, Doi
, N.
, Miyahara
, S.
, Shinoda
, Y.
, and Carpenter
, D. O.
(1991
). “Appearance of NMDA receptors triggered by anoxia independent of voltage in vivo and in vitro
,” Exp. Neurol.
112
, 304
–311
. 19.
Juiz
, J. M.
, Rueda
, J.
, Merchan
, J. A.
, and Sala
, M. L.
(1989
). “The effects of kainic acid on the cochlear ganglion of the rat
,” Hearing Res.
40
, 65
–74
. 20.
Kalb
, R. G.
, Lidow
, M. S.
, Halsted
, M. J.
, and Hockfield
, S.
(1992
). “N-methyl-D-aspartate receptors are transiently expressed in the developing spinal cord ventral horn
,” Proc. Natl. Acad. Sci. USA
89
, 8502
–8506
. 21.
Komuro
, H.
, and Rakic
, P.
(1993
). “Modulation of neuronal migration by NMDA receptors
,” Science
260
, 95
–97
. 22.
Lefebvre
, P. P.
, Malgrange
, B.
, Staecker
, H.
, Moghadass
, M.
, Van de Water
, T. R.
, and Moonen
, G.
(1994
). “Neurotrophins affect survival and neuritogenesis by adult injured auditory neurons in vitro
,” NeuroReport
5
, 865
–868
. 23.
Mayat
, E.
, Lebrun
, F.
, Sassetti
, I.
, and Recasens
, M.
(1994a
). “Ontogenesis of quisqualate-associated phosphoinositide metabolism in various regions of the rat nervous system
,” Int. J. Dev. Neurosci.
12
, 1
–17
. 24.
Mayat
, E.
, Larner-Natoli
, M.
, Rondouin
, G.
, Lebrun
, F.
, Sassetti
, I.
, and Reasens
, M.
(1994b
). “Kainate-induced status epilepticus leads to a delayed increase in various specific glutamate metabotropic receptor responses in the hippocampus
,” Brain Res.
645
, 186
–200
. 25.
McFadden
, E. A.
, and Saunders
, J. C.
(1989
). “Recovery of auditory function following intense sound exposure in the neonatal chick
,” Hearing Res.
41
, 205
–216
. 26.
Olney
, J. W.
(1986
). “Inciting excitotoxic cytocide among central neurons
,” Adv. Exp. Med. Biol.
203
, 631
–645
. 27.
Patuzzi
, R. B.
, and Bull
, C. L.
(1991
). “Electrical responses from the chicken basilar papilla
,” Hearing Res.
53
, 57
–77
. 28.
Powers
, N. L.
, Salvi
, R. J.
, Wang
, J.
, Spongr
, V.
, and Qiu
, C. X.
(1995
). “Elevation of auditory thresholds by spontaneous cochlear oscillations
,” Nature (London)
375
, 585
–587
. 29.
Puel
, J. L.
, d’Aldin
, C.
, Ruel
, J.
, Ladrech
, S.
, and Pujol
, R.
(1997
). “Synaptic repair mechanisms responsible for functional recovery in various cochlear pathologies
,” Acta Oto-Laryngol.
117
, 214
–218
. 30.
Puel
, J. L.
, Pujol
, R.
, Ladrech
, S.
, and Eybalin
, M.
(1991
). “Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid electrophysiological and neurotoxic effects in the guinea-pig cochlea
,” Neuroscience (NY)
45
, 63
–72
. 31.
Puel
, J. L.
, Pujol
, R.
, Tribillac
, F.
, Ladrech
, S.
, and Eybalin
, M.
(1994
). “Excitatory amino acid antagonists protect cochlear auditory neurons from excitotoxicity
,” J. Comp. Neurol.
341
, 241
–256
. 32.
Puel, J. L., Saffiedine, S., Gervais d’Aldin, C., Eybalin, M., and Pujol, R. (1995). “Synaptic regeneration and functional recovery after excitotoxic injury in the guinea pig cochlea,” Comptes Rendus de I Academic des Sciences—Serie Iii, Sciences de la Vie 318, pp. 67–75.
33.
Pujol, R., D’Aldin, G., Saffiedine, S., and Eybalin, M. (1996). “Repair of inner hair cell-auditory nerve synapses and recovery of function after an excitotoxic injury,” in Auditory Plasticity and Regeneration, edited by R. J. Salvi, D. Henderson, F. Fiorino, and V. Colletti (Thieme Medical, New York), pp. 100–107.
34.
Pujol
, R.
, Lenoir
, M.
, Robertson
, D.
, Eybalin
, M.
, and Johnstone
, B. M.
(1985
). “Kainic acid selectively alters auditory dendrites connected with cochlear inner hair cells
,” Hearing Res.
18
, 145
–151
. 35.
Pujol
, R.
, Rebillard
, G.
, Puel
, J. L.
, Lenoir
, M.
, Eybalin
, M.
, and Recasens
, M.
(1990
). “Glutamate neurotoxicity in the cochlea: a possible consequence of ischaemic or anoxic conditions occurring in ageing
,” Acta Oto-Laryngol. Suppl.
476
, 32
–36
. 36.
Rothman
, S. M.
(1985
). “The neurotoxicity of excitatory amino acids is produced by passive chloride influx
,” J. Neurosci.
5
, 1483
–1489
. 37.
Rubel, E. W. (1992). “Regeneration of hair cells in the avian inner ear,” in Noise Induced Hearing Loss, edited by A. Dancer, D. Henderson, R. J. Salvi, and R. P. Hamernik (Mosby Year Book, St. Louis), pp. 204–227.
38.
Ryals
, B. M.
, and Rubel
, E. W.
(1988
). “Hair cell regeneration after acoustic trauma in adult Coturnix quail
,” Science
240
, 1774
–1776
. 39.
Ryals
, B. M.
, Ten Eyck
, B.
, and Westbrook
, E. W.
(1989
). “Ganglion cell loss continues during hair cell regeneration
,” Hearing Res.
43
, 81
–90
. 40.
Ryals
, B. M.
, Westbrook
, E. W.
, Stoots
, S.
, and Spencer
, R. F.
(1992
). “Changes in the acoustic nerve after hair cell regeneration
,” Exp. Neurol.
115
, 18
–22
. 41.
Salvi
, R. J.
, Saunders
, S. S.
, Hashino
, E.
, and Chen
, L.
(1994
). “Discharge patterns of chicken cochlear ganglion neurons following kanamycin-induced hearing loss and regeneration
,” J. Comp. Physiol. A
174
, 351
–369
. 42.
Saunders
, S. S.
, Salvi
, R. J.
, and Miller
, K. M.
(1995
). “Recovery of thresholds and temporal integration in adult chickens after high-level 525-Hz pure-tone exposure
,” J. Acoust. Soc. Am.
97
, 1150
–1164
. 43.
Schuknecht
, H. F.
, and Woellner
, R. C.
(1953
). “Hearing losses following partial section of the cochlear nerve
,” Laryngoscope
63
, 441
–465
. 44.
Seren
, M. S.
, Aldinio
, C.
, Zanoni
, R.
, Leon
, A.
, and Nicoletti
, F.
(1989
). “Stimulation of inositol phospholipid hydrolysis by excitatory amino acids is enhanced in brain slices from vulnerable regions after transient global ischemia
,” J. Neurochem.
53
, 1700
–1705
. 45.
Shero
, M.
, Salvi
, R. J.
, Chen
, L.
, and Hashino
, E.
(1998
). “Excitotoxic effect of kainic acid on chicken cochlear afferent neurons
,” Neurosci. Lett.
257
, 81
–84
. 46.
Spoendlin, H. (1976). “Anatomical changes following various noise exposures,” in Effects of Noise on Hearing, edited by D. Henderson, R. P. Hamernik, J. Mills, and D. Dosanjh (Raven, New York), pp. 69–89.
47.
Trautwein
, P.
, Hofstetter
, P.
, Wang
, J.
, Salvi
, R.
, and Nostrant
, A.
(1996a
). “Selective inner hair cell loss does not alter distortion product otoacoustic emissions
,” Hearing Res.
96
, 71
–82
. 48.
Trautwein
, P.
, Salvi
, R. J.
, Miller
, K.
, Shero
, M.
, and Hashino
, E.
(1996b
). “Incomplete recovery of chicken distortion product otoacoustic emissions following acoustic overstimulation
,” Audiol. Neuro-Otol.
1
, 86
–103
. 49.
Wang
, J.
, Powers
, N. L.
, Hofstetter
, P.
, Trautwein
, P.
, Ding
, D.
, and Salvi
, R.
(1997
). “Effects of selective inner hair cell loss on auditory nerve fiber threshold, tuning and spontaneous and driven discharge rate
,” Hearing Res.
107
, 67
–82
. 50.
Zheng
, X. Y.
, Ding
, D. L.
, McFadden
, S. L.
, and Henderson
, D.
(1997a
). “Evidence that inner hair cells are the major source of cochlear summating potentials
,” Hearing Res.
113
, 76
–88
. 51.
Zheng
, X. Y.
, Henderson
, D.
, Hu
, B. H.
, and McFadden
, S. L.
(1997b
). “Recovery of structure and function of inner ear afferent synapses following kainic acid excitotoxicity
,” Hearing Res.
105
, 65
–76
. 52.
Zheng
, X. Y.
, McFadden
, S. L.
, and Henderson
, D.
(1998
). “Faster recovery in central than in peripheral auditory system following a reversible cochlear deafferentation
,” Neuroscience (NY)
85
, 579
–586
. 53.
Zheng
, X. Y.
, Wang
, J.
, Salvi
, R. J.
, and Henderson
, D.
(1996
). “Effects of kainic acid on the cochlear potentials and distortion product otoacoustic emissions in chinchilla
,” Hearing Res.
95
, 161
–167
.
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