Cultured Chinese hamster ovary cells attached to thin Mylar sheets were exposed to 3.3-MHz ultrasound in the presence of ultrasound contrast agent. The ultrasound beam was directed upward at the exposure chamber with the monolayer on the inside of the upper acoustic window. Cell membrane damage was detected by the firefly enzyme assay for released ATP and the subharmonic, fundamental, and second harmonic scattered signals were recorded. ATP release increased monotonically with increasing pressure amplitude above apparent thresholds of 0.28 MPa for 1-s continuous and 0.56 MPa for 100-s pulsed (10-μs pulses, 1-ms PRP) exposures with 5% The subharmonic signal and, to a lesser extent, the second harmonic signal both increased with the cell membrane damage, which suggests that these signals have predictive value for bioeffects. If the monolayer was positioned on the front window of the exposure chamber, cell membrane damage was greatly reduced, which confirms the protective influence of this configuration of monolayers reported in the literature. The effect decreased both at high (50%) or low (0.5%) concentrations of The strong nonlinear scattering of ultrasound by contrast agent gas bodies appears to provide useful indicators of gas body activity including cavitational bioeffects.
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February 1998
February 01 1998
The relationship of scattered subharmonic, 3.3-MHz fundamental and second harmonic signals to damage of monolayer cells by ultrasonically activated Available to Purchase
Douglas L. Miller;
Douglas L. Miller
MS# P7-53, Battelle Pacific Northwest National Laboratory, PO Box 999, Richland, Washington 99352
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Shiping Bao
Shiping Bao
U.S. Transuranium and Uranium Registries, Washington State University, Richland, Washington 99352
Search for other works by this author on:
Douglas L. Miller
MS# P7-53, Battelle Pacific Northwest National Laboratory, PO Box 999, Richland, Washington 99352
Shiping Bao
U.S. Transuranium and Uranium Registries, Washington State University, Richland, Washington 99352
J. Acoust. Soc. Am. 103, 1183–1189 (1998)
Article history
Received:
June 25 1997
Accepted:
October 08 1997
Citation
Douglas L. Miller, Shiping Bao; The relationship of scattered subharmonic, 3.3-MHz fundamental and second harmonic signals to damage of monolayer cells by ultrasonically activated . J. Acoust. Soc. Am. 1 February 1998; 103 (2): 1183–1189. https://doi.org/10.1121/1.421250
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