High-speed atomic force microscopy (HS-AFM) is a powerful tool for studying the dynamics of biomolecules in vitro because of its high temporal and spatial resolution. However, multi-functionalization, such as combination with complementary measurement methods, environment control, and large-scale mechanical manipulation of samples, is still a complex endeavor due to the inherent design and the compact sample scanning stage. Emerging tip-scan HS-AFM overcame this design hindrance and opened a door for additional functionalities. In this study, we designed a motor-driven stretching device to manipulate elastic substrates for HS-AFM imaging of biomolecules under controllable mechanical stimulation. To demonstrate the applicability of the substrate stretching device, we observed a microtubule buckling by straining the substrate and actin filaments linked by α-actinin on a curved surface. In addition, a BAR domain protein BIN1 that senses substrate curvature was observed while dynamically controlling the surface curvature. Our results clearly prove that large-scale mechanical manipulation can be coupled with nanometer-scale imaging to observe biophysical effects otherwise obscured.
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November 2022
Research Article|
November 04 2022
Tip-scan high-speed atomic force microscopy with a uniaxial substrate stretching device for studying dynamics of biomolecules under mechanical stress Available to Purchase
Feng-Yueh Chan
;
Feng-Yueh Chan
(Conceptualization, Formal analysis, Investigation, Methodology, Software, Writing – original draft)
1
Department of Physics, Nagoya University
, Chikusa-ku, Nagoya 464-8602, Japan
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Ryo Kurosaki;
Ryo Kurosaki
(Formal analysis, Investigation, Methodology)
1
Department of Physics, Nagoya University
, Chikusa-ku, Nagoya 464-8602, Japan
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Christian Ganser
;
Christian Ganser
(Funding acquisition, Investigation, Methodology, Software, Writing – review & editing)
2
Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences
, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan
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Tetsuya Takeda;
Tetsuya Takeda
(Funding acquisition, Investigation, Writing – review & editing)
3
Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
, Kita-Ku, Okayama 700-8558, Japan
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Takayuki Uchihashi
Takayuki Uchihashi
a)
(Conceptualization, Funding acquisition, Project administration, Software, Supervision)
1
Department of Physics, Nagoya University
, Chikusa-ku, Nagoya 464-8602, Japan
2
Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences
, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan
4
Institute for Glyco-core Research (iGCORE), Nagoya University
, Chikusa-ku, Nagoya 464-8602, Japan
a)Author to whom correspondence should be addressed: [email protected]
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Feng-Yueh Chan
1
Ryo Kurosaki
1
Christian Ganser
2
Tetsuya Takeda
3
Takayuki Uchihashi
1,2,4,a)
1
Department of Physics, Nagoya University
, Chikusa-ku, Nagoya 464-8602, Japan
2
Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences
, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan
3
Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
, Kita-Ku, Okayama 700-8558, Japan
4
Institute for Glyco-core Research (iGCORE), Nagoya University
, Chikusa-ku, Nagoya 464-8602, Japan
a)Author to whom correspondence should be addressed: [email protected]
Rev. Sci. Instrum. 93, 113703 (2022)
Article history
Received:
July 18 2022
Accepted:
October 09 2022
Citation
Feng-Yueh Chan, Ryo Kurosaki, Christian Ganser, Tetsuya Takeda, Takayuki Uchihashi; Tip-scan high-speed atomic force microscopy with a uniaxial substrate stretching device for studying dynamics of biomolecules under mechanical stress. Rev. Sci. Instrum. 1 November 2022; 93 (11): 113703. https://doi.org/10.1063/5.0111017
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