A superfluid helium insert was developed for cryogenic microscopy of millimeter-sized specimens. An optical-interferometric position sensor, cryogenic objective mirror, and piezo-driven cryogenic stage were fixed to an insert holder that was immersed in superfluid helium. The single-component design stabilized the three-dimensional position of the sample, with root-mean-square deviations of (x, lateral) 0.33 nm, (y, lateral) 0.29 nm, and (z, axial) 0.25 nm. Because of the millimeter working range of the optical sensor, the working range of the sample under the active stabilization was (x, y) 5 mm and (z) 3 mm in superfluid helium at 1.8 K. The insert was used to obtain the millimeter-sized fluorescence image of cell nuclei at 1.8 K without a sample exchange.
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October 2022
Research Article|
October 31 2022
Superfluid helium nanoscope insert with millimeter working range
Naoki Kamiya;
Naoki Kamiya
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft)
1
Department of Physics, Tokyo Institute of Technology
, Meguro, Tokyo 152-8550, Japan
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Kazuki Kuramoto;
Kazuki Kuramoto
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft)
1
Department of Physics, Tokyo Institute of Technology
, Meguro, Tokyo 152-8550, Japan
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Kento Takishima;
Kento Takishima
(Conceptualization, Investigation, Methodology, Writing – original draft)
1
Department of Physics, Tokyo Institute of Technology
, Meguro, Tokyo 152-8550, Japan
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Tatsuya Yumoto;
Tatsuya Yumoto
(Formal analysis, Investigation, Methodology, Writing – original draft)
1
Department of Physics, Tokyo Institute of Technology
, Meguro, Tokyo 152-8550, Japan
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Haruka Oda;
Haruka Oda
(Conceptualization, Investigation, Writing – original draft)
2
Institute of Innovative Research, Tokyo Institute of Technology
, Yokohama, Kanagawa 226-8503, Japan
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Takeshi Shimi;
Takeshi Shimi
(Conceptualization, Investigation, Methodology, Writing – original draft)
2
Institute of Innovative Research, Tokyo Institute of Technology
, Yokohama, Kanagawa 226-8503, Japan
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Hiroshi Kimura;
Hiroshi Kimura
(Conceptualization, Funding acquisition, Investigation, Methodology, Writing – original draft)
2
Institute of Innovative Research, Tokyo Institute of Technology
, Yokohama, Kanagawa 226-8503, Japan
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Michio Matsushita;
Michio Matsushita
(Conceptualization, Funding acquisition, Project administration, Writing – original draft)
1
Department of Physics, Tokyo Institute of Technology
, Meguro, Tokyo 152-8550, Japan
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Satoru Fujiyoshi
Satoru Fujiyoshi
a)
(Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Writing – original draft)
1
Department of Physics, Tokyo Institute of Technology
, Meguro, Tokyo 152-8550, Japan
a)Author to whom correspondence should be addressed: fujiyoshi@phys.titech.ac.jp
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a)Author to whom correspondence should be addressed: fujiyoshi@phys.titech.ac.jp
Rev. Sci. Instrum. 93, 103703 (2022)
Article history
Received:
July 04 2022
Accepted:
September 30 2022
Citation
Naoki Kamiya, Kazuki Kuramoto, Kento Takishima, Tatsuya Yumoto, Haruka Oda, Takeshi Shimi, Hiroshi Kimura, Michio Matsushita, Satoru Fujiyoshi; Superfluid helium nanoscope insert with millimeter working range. Rev. Sci. Instrum. 1 October 2022; 93 (10): 103703. https://doi.org/10.1063/5.0107395
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