We have developed a handling-free mounting method for X-ray crystallography of protein crystals at room temperatures—the glass capillary method. In this method, crystals were nucleated and grown on the capillary walls, and then, growth solutions were gently removed. The procedures for collecting data on the crystals were conducted by simply setting the capillary on the goniometer of a synchrotron beamline without touching the crystals. Crystal quality was characterized using mosaicity, resolution at I/σ(I) = 2, I/σ(I) at resolution = 2.0 Å, Rmerge, and completeness. Wilson plots were also used to characterize the quality of crystals. In particular, all samples showed very low mosaicity; the handling-free method successfully retained their low mosaicity and effectively maintained the crystal quality.
A novel handling-free method of mounting single protein crystals for synchrotron structure analyses at room temperature
Yoshihisa Suzuki, Kumi Torii, Koji Inaka, Takahisa Fujiwara, Hiroaki Tanaka, Yasutomo Arai; A novel handling-free method of mounting single protein crystals for synchrotron structure analyses at room temperature. Rev. Sci. Instrum. 1 May 2019; 90 (5): 054101. https://doi.org/10.1063/1.5070122
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