A novel Fourier-transform infrared (FT-IR) rapid-scan spectrometer has been developed (patent pending EP14194520.4) which yields 1000 times higher time resolution as compared to conventional rapid-scanning spectrometers. The central element to achieve faster scanning rates is based on a sonotrode whose front face represents the movable mirror of the interferometer. A prototype spectrometer with a time resolution of 13 μs was realized, capable of fully automated long-term measurements with a flow cell for liquid samples, here a photosynthetic membrane protein in solution. The performance of this novel spectrometer is demonstrated by recording the photoreaction of bacteriorhodopsin initiated by a short laser pulse that is synchronized to the data recording. The resulting data are critically compared to those obtained by step-scan spectroscopy and demonstrate the relevance of performing experiments on proteins in solution. The spectrometer allows for future investigations of fast, non-repetitive processes, whose investigation is challenging to step-scan FT-IR spectroscopy.
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June 2016
Research Article|
June 15 2016
New ultrarapid-scanning interferometer for FT-IR spectroscopy with microsecond time-resolution
B. Süss;
B. Süss
1Department of Physics, Experimental Molecular Biophysics,
Freie Universität Berlin
, Arnimallee 14, 14195 Berlin, Germany
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F. Ringleb;
F. Ringleb
a)
2
Institute for Crystal Growth
, Max-Born Straße 2, 12489 Berlin, Germany
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J. Heberle
J. Heberle
b)
1Department of Physics, Experimental Molecular Biophysics,
Freie Universität Berlin
, Arnimallee 14, 14195 Berlin, Germany
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a)
This research was performed while F. Ringleb was at Fritz Haber Institute of the Max Planck Society, Berlin 14195, Germany.
b)
Electronic mail: [email protected].
Rev. Sci. Instrum. 87, 063113 (2016)
Article history
Received:
March 15 2016
Accepted:
May 30 2016
Citation
B. Süss, F. Ringleb, J. Heberle; New ultrarapid-scanning interferometer for FT-IR spectroscopy with microsecond time-resolution. Rev. Sci. Instrum. 1 June 2016; 87 (6): 063113. https://doi.org/10.1063/1.4953658
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