It is possible to measure luminescence quantum yield in a facile way, by designing an optical spectrometer capable of obtaining electronic absorption as well as luminescence spectra, with a setup that uses the same light source and detector for both the spectral measurements. Employment of a single light source and single detector enables use of the same correction factor profile for spectral corrections. A suitable instrumental scaling factor is used for adjusting spectral losses.
REFERENCES
1.
C. A.
Parker
and W. T.
Rees
, Analyst
85
, 587
(1960
).2.
A. T.
Rhys williams
, S. A.
Winfield
, and J. N.
Miller
, Analyst
108
, 1067
(1983
).3.
S. I.
Vavilov
, Z. Phys.
22
, 266
(1924
).4.
G.
Weber
and F. W. J.
Teale
, Trans. Faraday Soc.
53
, 646
(1957
).5.
J. C.
De Mello
, H. F.
Wittmann
, and R. H.
Friend
, Adv. Mater.
9
, 230
(1997
).6.
A. M.
Brouwer
, Pure Appl. Chem.
83
, 2213
(2011
).7.
C.
Wurth
, M. G.
Gonzalez
, R.
Niessner
, U.
Panne
, C.
Haisch
, and U. R.
Genger
, Talanta
90
, 30
(2012
).8.
H.
Ishida
, S.
Tobita
, Y.
Hasegawa
, R.
Katoh
, and K.
Nozaki
, Coord. Chem. Rev.
254
, 2449
(2010
).9.
K.
Suzuki
, A.
Kobayashi
, S.
Kaneko
, K.
Takehira
, T.
Yoshihara
, H.
Ishida
, Y.
Shiina
, S.
Oishi
, and S.
Tobita
, Phys. Chem. Chem. Phys.
11
, 9850
(2009
).10.
Y.
Kawamura
, H.
Sasabe
, and C.
Adachi
, Jpn. J. Appl. Phys.
43
, 7729
(2004
).11.
K.
Rurack
, Springer Ser. Fluoresc.
5
, 101
(2008
).12.
W. R.
Ware
and B. A.
Baldwin
, J. Chem. Phys.
43
, 1194
(1965
).13.
M.
Born
and E.
Wolf
, Principles of Optics
(Pergamon
, Oxford
, 1969
; Nauka, Moscow, 1970).14.
I. V.
Antonets
, L. N.
Kotov
, V. G.
Shavrov
, and V. I.
Shcheglov
, J. Commun. Technol. Electron.
53
, 363
(2008
).15.
W. H.
Melhuish
, Pure Appl. Chem.
56
, 231
(1984
).16.
S. E.
Braslavsky
, Pure Appl. Chem.
79
, 293
(2007
).17.
S. T.
Flock
, S. L.
Jacques
, B. C.
Wilson
, W. M.
Star
, and M. J. C.
van Gemert
, Lasers Surg. Med.
12
, 510
(1992
).18.
P. M.
Epperson
and M. B.
Denton
, Anal. Chem.
61
, 1513
(1989
).19.
J. J.
Hermans
and S.
Levinson
, J. Opt. Soc. Am.
41
, 460
(1951
).20.
21.
J. N.
Demas
and G. A.
Crosby
, J. Phys. Chem.
75
, 991
(1971
).22.
J.
Rheims
, J.
Köser
, and T.
Wriedt
, Meas. Sci. Technol.
8
, 601
(1997
).23.
K.
Moutzouris
, M.
Papamichael
, S. C.
Betsis
, I.
Stavrakas
, G.
Hloupis
, and D.
Triantis
, Appl. Phys. B: Lasers Opt.
113
, 617
(2014
).24.
H.
El-Kashef
, Physica B
279
, 295
(2000
).25.
G. M.
Hale
and M. R.
Querry
, Appl. Opt.
12
, 555
(1973
).26.
W. R.
Dawson
and M. W.
Windsor
, J. Phys. Chem.
72
, 3251
(1968
).27.
L. S.
Rohwer
and J. E.
Martin
, J. Lumin.
115
, 77
(2005
).28.
R. F.
Kubin
and A. N.
Fletcher
, Chem. Phys. Lett.
99
, 49
(1983
).29.
See supplementary material at http://dx.doi.org/10.1063/1.4940234 for absorption and emission spectra of fluorescein in 0.1N NaOH at various monochromatic excitations measured using single beam UV-VIS spectrometer, concentrations, and UV-VIS spectra of selected fluorophores, absorption, and emission spectra of selected fluorophores at monochromatic excitation measured using single beam UV-VIS spectrometer and measured quantum yield of the fluorophores in different media.
© 2016 AIP Publishing LLC.
2016
AIP Publishing LLC
You do not currently have access to this content.
