New developments in the field of microscopy enable to acquire increasing amounts of information from large sample areas and at an increased resolution. Depending on the nature of the technique, the information may reveal morphological, structural, chemical, and still other sample characteristics. In research fields, such as cell biology and materials science, there is an increasing demand to correlate these individual levels of information and in this way to obtain a better understanding of sample preparation and specific sample properties. To address this need, integrated systems were developed that combine nanometer resolution electron microscopes with optical microscopes, which produce chemically or label specific information through spectroscopy. The complementary information from electron microscopy and light microscopy presents an opportunity to investigate a broad range of sample properties in a correlated fashion. An important part of correlating the differences in information lies in bridging the different resolution and image contrast features. The trend to analyse samples using multiple correlated microscopes has resulted in a new research field. Current research is focused, for instance, on (a) the investigation of samples with nanometer scale distribution of inorganic and organic materials, (b) live cell analysis combined with electron microscopy, and (c) in situ spectroscopic and electron microscopy analysis of catalytic materials, but more areas will benefit from integrated correlative microscopy.
Skip Nav Destination
,
Article navigation
January 2015
Review Article|
January 15 2015
Contributed Review: Review of integrated correlative light and electron microscopy
F. J. Timmermans;
F. J. Timmermans
Medical Cell Biophysics Group, MIRA Institute,
University of Twente
, P.O. Box 217, 7500 AE Enschede, The Netherlands
Search for other works by this author on:
C. Otto
C. Otto
Medical Cell Biophysics Group, MIRA Institute,
University of Twente
, P.O. Box 217, 7500 AE Enschede, The Netherlands
Search for other works by this author on:
F. J. Timmermans
C. Otto
Medical Cell Biophysics Group, MIRA Institute,
University of Twente
, P.O. Box 217, 7500 AE Enschede, The Netherlands
Rev. Sci. Instrum. 86, 011501 (2015)
Article history
Received:
May 22 2014
Accepted:
December 20 2014
Citation
F. J. Timmermans, C. Otto; Contributed Review: Review of integrated correlative light and electron microscopy. Rev. Sci. Instrum. 1 January 2015; 86 (1): 011501. https://doi.org/10.1063/1.4905434
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
Citing articles via
Overview of the early campaign diagnostics for the SPARC tokamak (invited)
M. L. Reinke, I. Abramovic, et al.
Automated polarization rotation for multi-axis rotational-anisotropy second harmonic generation experiments
Karna A. Morey, Bryan T. Fichera, et al.
Measurement setup for the characterization of integrated semiconductor circuits at cryogenic temperatures
P. J. Ritter, M.-A. Tucholke, et al.
Related Content
Focus on nanoscience and nanotechnology
Physics Today (April 2010)