The construction of a microscope with fast sample transfer system for single-molecule spectroscopy and microscopy at low temperatures using 2D/3D sample-scanning is reported. The presented construction enables the insertion of a sample from the outside (room temperature) into the cooled (4.2 K) cryostat within seconds. We describe the mechanical and optical design and present data from individual Photosystem I complexes. With the described setup numerous samples can be investigated within one cooling cycle. It opens the possibility to investigate biological samples (i) without artifacts introduced by prolonged cooling procedures and (ii) samples that require preparation steps like plunge-freezing or specific illumination procedures prior to the insertion into the cryostat.
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December 2012
Research Article|
December 12 2012
Confocal sample-scanning microscope for single-molecule spectroscopy and microscopy with fast sample exchange at cryogenic temperatures
Martin Hussels;
Martin Hussels
Universität Tübingen
, Institut für Physikalische und Theoretische Chemie and LISA+ Center, Auf der Morgenstelle 18, 72076 Tübingen, Germany
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Alexander Konrad;
Alexander Konrad
Universität Tübingen
, Institut für Physikalische und Theoretische Chemie and LISA+ Center, Auf der Morgenstelle 18, 72076 Tübingen, Germany
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Marc Brecht
Marc Brecht
a)
Universität Tübingen
, Institut für Physikalische und Theoretische Chemie and LISA+ Center, Auf der Morgenstelle 18, 72076 Tübingen, Germany
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Martin Hussels
Alexander Konrad
Marc Brecht
a)
Universität Tübingen
, Institut für Physikalische und Theoretische Chemie and LISA+ Center, Auf der Morgenstelle 18, 72076 Tübingen, Germany
a)
Telephone: +49-7071-29-76239. Fax: +49-7071-29-5490. E-mail: [email protected].
Rev. Sci. Instrum. 83, 123706 (2012)
Article history
Received:
June 28 2012
Accepted:
November 19 2012
Citation
Martin Hussels, Alexander Konrad, Marc Brecht; Confocal sample-scanning microscope for single-molecule spectroscopy and microscopy with fast sample exchange at cryogenic temperatures. Rev. Sci. Instrum. 1 December 2012; 83 (12): 123706. https://doi.org/10.1063/1.4769996
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