We have developed optical tweezers, with force measurements based on fast video tracking, for analysis and control of DNA translocation through nanocapillaries. Nanocapillaries are single-molecule biosensors with very similar characteristics to solid-state nanopores. Our novel experimental setup allows for ionic-current measurements in which the nanocapillary is oriented perpendicular to the trapping laser. Using video-based particle tracking, we are able to measure the position of DNA coated colloids at sub-millisecond resolution and in real-time. We present the first electrophoretic force and simultaneous ionic-current measurements of a single DNA molecule inside the orifice of a nanocapillary.
Note: Direct force and ionic-current measurements on DNA in a nanocapillary
O. Otto, L. J. Steinbock, D. W. Wong, J. L. Gornall, U. F. Keyser; Note: Direct force and ionic-current measurements on DNA in a nanocapillary. Rev. Sci. Instrum. 1 August 2011; 82 (8): 086102. https://doi.org/10.1063/1.3623502
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