This paper presents the design and implementation of a three-axis steering system, wherein a micro/nanoparticle is optically trapped and propelled to serve as a measurement probe. The actuators in the system consist of a deformable mirror enabling axial steering and a two-axis acousto-optic deflector for lateral steering. The actuation range is designed and calibrated to be over 20μm along the two lateral axes and over 10μm along the axial direction. The actuation bandwidth of the two lateral axes is over 50 kHz and the associated resolution is 0.016 nm (1σ). The axial resolution is 0.16 nm, while the bandwidth is enhanced to over 3 kHz by model cancellation method. The performance of the three-axis steering system is illustrated by three sets of experiments. First, active Brownian motion control of the trapped probe is utilized to enhance trapping stability. Second, a large range three-dimensional (3D) steering of a 1.87μm probe, contouring a complex 3D trajectory in a 6×6×4μm3 volume, is demonstrated. Third, a closed-loop steering is implemented to achieve improved precision.

1.
A.
Ashkin
,
J. M.
Dziedzic
,
J. E.
Bjorkholm
, and
S.
Chu
,
Opt. Lett.
11
,
288
(
1986
).
2.
A.
Ashkin
,
J. M.
Dziedzic
, and
T.
Yamane
,
Nature (London)
330
,
769
(
1987
).
3.
M. D.
Wang
,
M. J.
Schnitzer
,
H.
Yin
,
R.
Landick
,
J.
Gelles
, and
S. M.
Block
,
Science
282
,
902
(
1998
).
4.
B.
Brower-Toland
,
D. A.
Wacker
,
R. M.
Fulbright
,
J. T.
Lis
,
W. L.
Kraus
, and
M. D.
Wang
,
J. Mol. Biol.
346
,
135
(
2005
).
5.
K. C.
Neuman
and
S. M.
Block
,
Rev. Sci. Instrum.
75
,
2787
(
2004
).
6.
I. M.
Peters
,
B. G.
de Grooth
,
J. M.
Schins
,
C. G.
Figdor
, and
J.
Greve
,
Rev. Sci. Instrum.
69
,
2762
(
1998
).
7.
A.
Pralle
,
M.
Prummer
,
E. -L.
Florin
,
E. H. K.
Stelzer
, and
J. K. H.
Horber
,
Microsc. Res. Tech.
44
,
378
(
1999
).
8.
A.
Rohrbach
and
E. H. K.
Stelzer
,
J. Appl. Phys.
91
,
5474
(
2002
).
9.
A.
Rohrbach
,
H.
Kress
, and
E. H. K.
Stelzer
,
Opt. Lett.
28
,
411
(
2003
).
10.
J. K.
Dreyer
,
K.
Berg-Sorensen
, and
L.
Oddershede
,
Appl. Opt.
43
,
1991
(
2004
).
11.
M. J.
Lang
,
C. L.
Asbury
,
J. W.
Shaevitz
, and
S. M.
Block
,
Biophys. J.
83
,
491
(
2002
).
12.
A.
Rohrbach
,
C.
Tischer
,
D.
Neumayer
,
E. -L.
Florin
, and
E. H. K.
Stelzer
,
Rev. Sci. Instrum.
75
,
2197
(
2004
).
13.
C.
Bustamante
,
J. C.
Macosko
, and
G. J.
Wuite
,
Nat. Rev. Mol. Cell Biol.
1
,
130
(
2000
).
14.
M. D.
Wang
,
Curr. Opin. Biotechnol.
10
,
81
(
1999
).
15.
P. M.
Hansen
and
L.
Oddershede
,
Proc. SPIE
5930
,
1
(
2005
).
16.
A.
Sischka
,
R.
Eckel
,
K.
Toensing
,
R.
Ros
, and
D.
Anelmetti
,
Rev. Sci. Instrum.
74
,
4827
(
2003
).
17.
T.
Pilizota
,
T.
Bilyard
,
F.
Bai
,
M.
Futai
,
H.
Hosokawa
, and
R. M.
Berry
,
Biophys. J.
93
,
264
(
2007
).
18.
K. D.
Wulff
,
D. G.
Cole
, and
R. L.
Clark
,
Appl. Opt.
46
,
4923
(
2007
).
19.
K. C.
Vermeulen
,
J.
van Mameren
,
G. J. M.
Stienen
,
E. J. G.
Peterman
,
G. J. L.
Wuite
, and
C. F.
Schmidt
,
Rev. Sci. Instrum.
77
,
013704
(
2006
).
20.
R. M.
Simmons
,
J. T.
Finer
,
S.
Chu
, and
J. A.
Spudich
,
Biophys. J.
70
,
1813
(
1996
).
21.
A. E.
Wallin
,
H.
Ojala
,
E.
Haggstrom
, and
R.
Tuma
,
Appl. Phys. Lett.
92
,
224104
(
2008
).
22.
M. T.
Valentine
,
N. R.
Guydosh
,
B.
Gutierrez-Medina
,
A. N.
Fehr
,
J. O.
Andreasson
, and
S. M.
Block
,
Opt. Lett.
33
,
599
(
2008
).
23.
NanoTracker™ product brochure, JPK Instruments AG, http://www.jpk.com/nanotracker-tm.387.html.
24.
T.
Ota
,
S.
Kawata
,
T.
Sugiura
,
M. J.
Booth
,
M. A. A.
Neil
,
R.
Juskaitis
, and
T.
Wilson
,
Opt. Lett.
28
,
465
(
2003
).
25.
F.
Gittes
and
C. F.
Schmidt
,
Opt. Lett.
23
,
7
(
1998
).
26.
A. R.
Carter
,
G. M.
King
,
T. A.
Ulrich
,
W.
Halsey
,
D.
Alchenberger
, and
T. T.
Perkins
,
Appl. Opt.
46
,
421
(
2007
).
27.
Z.
Zhang
and
C. -H.
Menq
,
Appl. Opt.
47
,
2361
(
2008
).
28.
Z.
Zhang
and
C. -H.
Menq
,
Rev. Sci. Instrum.
78
,
083107
(
2007
).
29.
A.
Nussbaum
,
Optical System Design
(
Prentice-Hall
,
Englewood Cliffs, NJ
,
1998
).
30.
R. M.
Mazo
,
Brownian Motion: Fluctuations, Dynamics, and Applications
(
Clarendon
,
Oxford
,
2002
).
31.
See EPAPS supplementary material at http://dx.doi.org/10.1063/1.3156838 for the movies of corresponding steering.
32.
Adaptive optics guide, Flexible Optical B.V., http://www.okotech.com/catalogue.
33.
G. F.
Franklin
,
J. D.
Powell
, and
A.
Emami-Naeini
,
Feedback Control of Dynamic Systems
, 3rd ed. (
Addison-Wesley
,
Reading, MA
,
1994
).
34.
Y.
Jeong
,
G. R.
Jayanth
, and
C. -H.
Menq
,
Rev. Sci. Instrum.
78
,
093706
(
2007
).
35.
E. J. G.
Peterman
,
F.
Gittes
, and
C. F.
Schmidt
,
Biophys. J.
84
,
1308
(
2003
).
36.
Y.
Seol
,
A. E.
Carpenter
, and
T. T.
Perkins
,
Opt. Lett.
31
,
2429
(
2006
).
37.
K. C.
Neuman
,
E. H.
Chadd
,
G. F.
Liou
,
K.
Bergman
, and
S. M.
Block
,
Biophys. J.
77
,
2856
(
1999
).
38.
S.
Ayano
,
Y.
Wakamoto
,
S.
Yamashita
, and
K.
Yasuda
,
Biochem. Biophys. Res. Commun.
350
,
678
(
2006
).
39.
M. B.
Rasmussen
,
L. B.
Oddershede
, and
H.
Siegumfeldt
,
Appl. Environ. Microbiol.
74
,
2441
(
2008
).

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