A simple and inexpensive absorption technique for determination of calcium ion in urine samples is developed, comprising a light emitting diode (650 nm) as the light source and photodiode as the detector with AT89C51RD2 microcontroller. The design of the system and details of interface, calibration, and procedure of operation are explained in this paper. Software is developed to monitor sample processing and to display the results in liquid crystal display screen. With sample volume, a linear output is obtained in the range of 2.5–7.5 mM calcium with a detection limit of 0.06 mM. Interferences from other cations such as monovalent ion and divalent ion are investigated in the expected range, which are normally present in clinical samples, and absorption changes over the range of 3–12 are also determined. This system has been demonstrated successfully for the successive assay of calcium in urine samples, with the results comparing well to those achieved and in good agreement with values obtained with the current clinical spectrophotometric method at 95% of confidence level.
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April 2009
Research Article|
April 24 2009
Measurement of urinary calcium using AT89C51RD2 microcontroller Available to Purchase
P. Neelamegam;
P. Neelamegam
a)
1Department of Electronics and Instrumentation Engineering,
SASTRA University
, Thanjavur 613 402, Tamil Nadu, India
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A. Jamaludeen;
A. Jamaludeen
b)
2Department of Electronics,
St. Joseph’s College (Autonomous)
, Tiruchirappalli 620 002, Tamil Nadu, India
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A. Rajendran;
A. Rajendran
c)
3PG and Research Department of Physics,
Nehru Memorial College (Autonomous)
, Puthanampatti, Tiruchirappalli 621 007, Tamil Nadu, India
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R. Raghunathan
R. Raghunathan
4PG and Research Department of Physics,
AVVM Sri Pushpam College (Autonomous)
, Poondi, Thanjavur, Tamil Nadu 613 503, India
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P. Neelamegam
1,a)
A. Jamaludeen
2,b)
A. Rajendran
3,c)
R. Raghunathan
4
1Department of Electronics and Instrumentation Engineering,
SASTRA University
, Thanjavur 613 402, Tamil Nadu, India
2Department of Electronics,
St. Joseph’s College (Autonomous)
, Tiruchirappalli 620 002, Tamil Nadu, India
3PG and Research Department of Physics,
Nehru Memorial College (Autonomous)
, Puthanampatti, Tiruchirappalli 621 007, Tamil Nadu, India
4PG and Research Department of Physics,
AVVM Sri Pushpam College (Autonomous)
, Poondi, Thanjavur, Tamil Nadu 613 503, India
a)
Electronic mail: [email protected].
b)
Electronic mail: [email protected].
c)
Electronic mail: [email protected].
Rev. Sci. Instrum. 80, 044704 (2009)
Article history
Received:
October 15 2008
Accepted:
March 17 2009
Citation
P. Neelamegam, A. Jamaludeen, A. Rajendran, R. Raghunathan; Measurement of urinary calcium using AT89C51RD2 microcontroller. Rev. Sci. Instrum. 1 April 2009; 80 (4): 044704. https://doi.org/10.1063/1.3115211
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