The construction of a multiphoton laser scanning microscope based on a dual-axis acousto-optic deflector (AOD) is described. Without mechanical inertia and physical movement of the scanner, this system has a high random-addressing and data-acquisition rate of . By proper compensation of the dispersion of the AODs, the spatial resolution of the system is for lateral direction and for axial direction. Fluorescence images are acquired to demonstrate these imaging performances.
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