In this article, a micro-organism tracking system using a high-speed vision system is reported. This system two dimensionally tracks a freely swimming micro-organism within the field of an optical microscope by moving a chamber of target micro-organisms based on high-speed visual feedback. The system we developed could track a paramecium using various imaging techniques, including bright-field illumination, dark-field illumination, and differential interference contrast, at magnifications of 5 times and 20 times. A maximum tracking duration of 300s was demonstrated. Also, the system could track an object with a velocity of up to 35 000μms(175diameterss), which is significantly faster than swimming micro-organisms.

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See EPAPS Document No. E-RSINAK-76-208503 for the movie file of the tracking experiments. A direct link to this document may be found in the online article’s HTML reference section. The document may also be reached via the EPAPS homepage (http:∕∕www.aip.org∕pubservs∕epaps.html) or from ftp.aip.org in the directory ∕epaps. See the EPAPS homepage for more information.

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