An instrument for the detection of optical signals from microfluidic biochips is described. The light detection system uses a LabView™-controlled photomultiplier tube with a programmable gain of Plastic optical fibers (POFs) of 1 mm diameter are used to deliver light to and from the microfluidic systems. The detection system is demonstrated by detecting fluorescence from 15 μm polystyrene spheres in commercial biochips and micropipettes using a custom POF launch and detect tip. The spatial response of the tip allows dynamic measurements of the velocities of the microparticles to be made.
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