The ability to manipulate environmental conditions while obtaining nanoscale information about a sample is invaluable. The current article describes the construction and use of a chamber to control the relative humidity while using the scanning probe microscope (SPM) to monitor changes in sample stiffness. Although the experimental setup described is simple, this work shows that the complex relationship between temperature and relative humidity needs to be fully understood for successful operation of the apparatus. In TappingMode™, increases in relative humidity caused the free amplitude of the oscillating cantilever to decrease. Force–distance measurements taken on glass, however, showed no changes in detector sensitivity, suggesting that force–distance mode could be used to measure changes sample stiffness as a function of relative humidity. Using the SPM in force–distance mode, three moisture-sensitive materials (gelatine, wool fibers, and cotton fibers), were examined as the relative humidity was gradually increased from ambient conditions to just under 100%. The results showed that each of the materials softened as the humidity increased and that the experiments were reproducible. From the data, it was estimated that gelatine passed through a glass transition at about 86% relative humidity at room temperature. Wool passed through a glass transition at about 85% relative humidity, which is consistent with previous findings.
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October 2002
Research Article|
October 01 2002
Using the scanning probe microscope to measure the effect of relative humidity on sample stiffness
J. M. Maxwell;
J. M. Maxwell
School of Chemistry, University of Melbourne, Parkville, Victoria 3010, Australia
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M. G. Huson
M. G. Huson
CSIRO Textile and Fibre Technology, Belmont, Victoria 3216, Australia
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Rev. Sci. Instrum. 73, 3520–3524 (2002)
Article history
Received:
May 29 2002
Accepted:
July 16 2002
Citation
J. M. Maxwell, M. G. Huson; Using the scanning probe microscope to measure the effect of relative humidity on sample stiffness. Rev. Sci. Instrum. 1 October 2002; 73 (10): 3520–3524. https://doi.org/10.1063/1.1505663
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