In this note, we present a setup which can be adapted to commercially available atomic force microscopes (Nanoscope II and III) to permit the observation of living cells in nearly physiological conditions. The setup permits one to heat the sample up to 40 °C, to exchange (without the use of the “O” ring) the imaging buffer while measuring, and to distinguish fluorescently labeled cell subpopulations.

1.
G.
Binnig
,
C.
Quate
, and
C.
Gerber
,
Phys. Rev. Lett.
56
,
930
(
1986
).
2.
S.
Kasas
,
V.
Gotzos
, and
M. R.
Celio
,
Biophys. J.
64
,
539
(
1993
).
3.
Y. G.
Kuznetsov
,
A. J.
Malkin
, and
A.
McPherson
,
J. Struct. Biol.
120
,
180
(
1997
).
4.
C.
Le Grimellec
,
E.
Lesniewska
,
M. C.
Giocondi
,
E.
Finot
,
V.
Vie
, and
J. P.
Goudonnet
,
Biophys. J.
75
,
695
(
1998
).
5.
M.
Radmacher
,
IEEE Eng. Med. Biol. Mag.
16
,
47
(
1997
).
6.
G. R.
Bushell
,
C.
Cahill
,
F. M.
Clarke
,
C. T.
Gibson
,
S.
Myhra
, and
G. S.
Watson
,
Cytometry
36
,
254
(
1999
).
7.
C.
Rotsch
and
M.
Radmacher
,
Biophys. J.
78
,
520
(
2000
).
8.
F.
Oulevey
,
G.
Gremaud
,
A. J.
Kulik
, and
B.
Guisolan
,
Rev. Sci. Instrum.
70
,
1889
(
1999
).
9.
T. R.
Baekmark
,
T.
Bjo/rnholm
, and
O. G.
Mouritsen
,
Rev. Sci. Instrum.
68
,
140
(
1997
).
10.
T.
Wade
,
J. F.
Garst
, and
J. L.
Stickney
,
Rev. Sci. Instrum.
70
,
121
(
1999
).
11.
J.
Mou
,
G.
Huang
, and
Z.
Shao
,
Rev. Sci. Instrum.
67
,
2654
(
1996
).
12.
www.nanotechtools.com.
13.
Spectra-Physics, 1330 Terra Bella 7013, Mountain View, CA 94039-7013.
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