Theory predicts that small angle light scattering by spherical particles of 5 to 20 μ diam is nearly proportional to volume and insensitive to particle refractive index. A flow system photometer with helium‐neon laser light source measures the scattering between 0.5 and 2.0° from individual particles at 104 to 105/min. Volume distributions of mammalian cells and plastic microspheres agree with other independent determinations.
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© 1969 The American Institute of Physics.
1969
The American Institute of Physics
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