Recent progress in atmospheric plasmas has led to the creation of cold plasmas with ion temperature close to room temperature. This paper outlines recent progress in understanding of cold plasma physics as well as application of cold atmospheric plasma (CAP) in cancer therapy. Varieties of novel plasma diagnostic techniques were developed recently in a quest to understand physics of CAP. It was established that the streamer head charge is about 108 electrons, the electrical field in the head vicinity is about 107 V/m, and the electron density of the streamer column is about 1019 m−3. Both in-vitro and in-vivo studies of CAP action on cancer were performed. It was shown that the cold plasma application selectively eradicates cancer cells in-vitro without damaging normal cells and significantly reduces tumor size in-vivo. Studies indicate that the mechanism of action of cold plasma on cancer cells is related to generation of reactive oxygen species with possible induction of the apoptosis pathway. It is also shown that the cancer cells are more susceptible to the effects of CAP because a greater percentage of cells are in the S phase of the cell cycle.
REFERENCES
1.
Plasma Medicine: Applications of Low-Temperature Gas Plasmas in Medicine and Biology
, edited by M.
Laroussi
, M.
Kong
, G.
Morfill
, and W.
Stolz
(Cambridge
, 2012
).2.
E.
Stoffels
, I. E.
Kieft
, R. E. J.
Sladek
, L. J. M.
van den Bedem
, E. P.
van der Laan
, and M.
Steinbuch
, “Plasma needle for in vivo medical treatment: Recent developments and perspectives
,” Plasma Sources Sci. Technol.
15
, S169
–S180
(2006
).3.
G.
Fridman
, G.
Friedman
, A.
Gutsol
, A. B.
Shekhter
, V. N.
Vasilets
, and A.
Fridman
, “Applied plasma medicine
,” Plasma Processes Polym.
5
, 503
(2008
).4.
See http://usmedinnovations.com for Argon Plasma Coagulator application in medicine.
5.
J.
Canady
, K.
Wiley
, and B.
Ravo
, “Argon plasma coagulation and the future applications for dual-mode endoscopic probes
,” Rev. Gastroenterological Disord.
6
, 1
(2006
).6.
E.
Stoffels
, A. J.
Flikweert
, W. W.
Stoffels
, and G. M. W.
Kroesen
, “Plasma needle: A non-destructive atmospheric plasma source for fine surface treatment of (bio)materials
,” Plasma Source Sci. Technol.
11
, 383
(2002
).7.
M.
Laroussi
and X.
Lu
, “Room-temperature atmospheric pressure plasma plume for biomedical applications
,” Appl. Phys. Lett.
87
, 113902
(2005
).8.
M.
Laroussi
, W.
Hynes
, T.
Akan
, X.
Lu
, and C.
Tendero
, “The plasma pencil: A source of hypersonic cold plasma bullets for biomedical applications
,” IEEE Trans. Plasma Sci.
36
, 1298
(2008
).9.
X.
Lu
, Z.
Jiang
, Q.
Xiong
, Z.
Tang
, and Y.
Pan
, “A single electrode room-temperature plasma jet device for biomedical applications
,” Appl. Phys. Lett.
92
, 151504
(2008
).10.
X.
Lu
, Z.
Jiang
, Q.
Xiong
, Z.
Tang
, X.
Hu
, and Y.
Pan
, “An 11 cm long atmospheric pressure cold plasma plume for applications of plasma medicine
,” Appl. Phys. Lett.
92
, 081502
(2008
).11.
J. F.
Kolb
, A. A. H.
Mohamed
, R. O.
Price
, R. J.
Swanson
, A.
Bowman
, R. L.
Chiavarini
, M.
Stacey
, and K. H.
Schoenbach
, “Cold atmospheric pressure air plasma jet for medical applications
,” Appl. Phys. Lett.
92
, 241501
(2008
).12.
G.
Fridman
, A.
Shereshevsky
, M.
Peddinghaus
, A.
Gutsol
, V.
Vasilets
, A.
Brooks
, M.
Balasubramanian
, G.
Friedman
, and A.
Fridman
, “Bio-medical applications of non-thermal atmospheric pressure plasma
,” in 37th AIAA Plasma dynamics and Lasers Conference
, San Francisco, California, 5–8 June, 2006
, AIAA-2006-2902.13.
G.
Fridman
, D.
Dobrynin
, S.
Kalghatgi
, A.
Brooks
, G.
Friedman
, A.
Fridman
, “Physical and biological mechanisms of plasma interaction with living tissue
,” in 36th International Conference Plasma Science
, San Diego, May 30–June 5, 2009
.14.
X.
Yan
, Z.
Xiong
, F.
Zou
, S.
Zhao
, X.
Lu
, G.
Yang
, G.
He
, and K.
Ostrikov
, “Plasma-induced death of HepG2 cancer cells: intracellular effects of reactive species
,” Plasma Processes Polym.
9
, 59
–66
(2012
).15.
X.
Pei
, X.
Lu
, J.
Liu
, D.
Liu
, Y.
Yang
, K.
Ostrikov
, P. K.
Chu
, and Y.
Pan
, J. Phys. D: Appl. Phys.
45
, 165205
(2012
).16.
S.
Kalghatgi
, A.
Fridman
, G.
Friedman
, and A.
Morss-Clyne
, “Non-thermal plasma enhances endothelial cell proliferation through fibroblast growth factor-2 release
,” in 36th International Conference Plasma Science
, San Diego, May 30–June 5, 2009
.17.
M. G.
Kong
, G.
Kroesen
, G.
Morfill
, T.
Nosenko
, T.
Shimizu
, J.
van Dijk
, and J. L.
Zimmermann
, “Plasma medicine. An introductory review
,” New J. Phys.
11
, 115012
(2009
).18.
G. E.
Morfill
, M. G.
Kong
, and J. L.
Zimmermann
, “Focus on plasma medicine. Review
,” New J. Phys.
11
, 115011
(2009
).19.
E.
Stoffels
, Y.
Sakiyama
, and D. B.
Graves
, “Cold atmospheric plasma: Charged species and their interactions with cells and tissues
,” IEEE Trans. Plasma Sci.
36
(4
), 1441
(2008
).20.
N.
Barekzi
and M.
Laroussi
, J. Phys. D: Appl. Phys.
45
, 422002
(2012
).21.
N.
Georgescu
and A. R.
Lupu
, “Tumoral and normal cells treatment with high-voltage pulsed cold atmospheric plasma jets
,” IEEE Trans. Plasma Sci.
38
, 1
(2010
).22.
J. L.
Zirnheld
, S. N.
Zucker
, T. M.
DiSanto
, R.
Berezney
, and K.
Etemadi
, “Nonthermal plasma needle: Development and targeting of melanoma cells
,” IEEE Trans. Plasma Sci.
38
, 948
(2010
).23.
A.
Shashurin
, M.
Keidar
, S.
Bronnikov
, R. A.
Jurjus
, and M. A.
Stepp
, Appl. Phys. Lett.
92
, 181501
(2008
).24.
O.
Volotskova
, M. A.
Stepp
, and M.
Keidar
, “Integrin activation by a cold atmospheric plasma jet
,” New J. Phys.
14
, 053019
(2012
).25.
M.
Vandamme
, E.
Robert
, S.
Pesnel
, E.
Barbosa
, S.
Dozias
, J.
Sobilo
, S.
Lerondel
, A.
Le Pape
, and J. M.
Pouvesle
, “Antitumor effect of plasma treatment on U87 glioma xenografts: Preliminary results
,” Plasma Processes Polym.
7
, 264
(2010
).26.
M.
Keidar
, R.
Walk
, A.
Shashurin
, P.
Srinivasan
, A.
Sandler
, S.
Dasgupta
, R.
Ravi
, R.
Guerrero-Preston
, and B.
Trink
, “Cold plasma selectivity and the possibility of a paradigm shift in cancer therapy
,” Br. J. Cancer
105
, 1295
(2011
).27.
M.
Vandamme
, E.
Robert
, S.
Lerondel
, V.
Sarron
, D.
Ries
, S.
Dozias
, J.
Sobilo
, D.
Gosset
, C.
Kieda
, B.
Legrain
, J.-M.
Pouvesle
, and A.
Le Pape
, “ROS implication in a new antitumor strategy based on non-thermal plasma
,” Int. J. Cancer
130
, 2185
(2011
).28.
G.
Isbary
, G.
Morfill
, H.-U.
Schmidt
, M.
Georgi
, K.
Ramrath
, J.
Heinlin
, S.
Karrer
, M.
Landthaler
, T.
Shimizu
, B.
Steffes
, W.
Bunk
, R.
Monetti
, J. L.
Zimmermann
, R.
Pompl
, and W.
Stolz
, “A first prospective randomized controlled trial to decrease bacterial load using cold atmospheric argon plasma onchronic wounds in patients
,” Br. J. Dermatol.
163
, 78
(2010
).29.
K.
Kim
, J. D.
Choi
, Y. C.
Hong
, G.
Kim
, F. J.
Noh
, J.-S.
Lee
, and S. S.
Yang
, “Atmospheric-pressure plasma-jet from micronozzle array and its biological effects on living cells for cancer therapy
,” Appl. Phys. Lett.
98
, 073701
(2011
).30.
G. E.
Morfill1
, T.
Shimizu
, B.
Steffes
, and H.-U.
Schmidt
, “Nosocomial infections—A new approach towards preventive medicine using plasmas
,” New Journal of Physics
11
, 115019
(2009
).31.
W. J. M.
Brok
, M. D.
Bowden
, J.
van Dijk
, J. J. A. M.
van der Mullen
, and G. M. W.
Kroesen
, “Numerical description of discharge characteristics of the plasma needle
,” J. Appl. Phys.
98
, 013302
(2005
).32.
Y.
Sakiyama
and D. B.
Graves
, “Finite element analysis of an atmospheric pressure RF-excited plasma needle
,” J. Phys. D, Appl. Phys.
39
, 3451
(2006
).33.
Y.
Sakiyama
and D. B.
Graves
, “Nonthermal atmospheric rf plasma in one-dimensional spherical coordinates: Asymmetric sheath structure and the discharge mechanism
,” J. Appl. Phys.
101
, 073306
(2007
).34.
F.
Shi
, D.
Wang
, and C.
Ren
, “Simulations of atmospheric pressure discharge in a high-voltage nanosecond pulse using the particle-in-cell Monte Carlo collision model in noble gases
,” Phys. Plasmas
15
, 063503
(2008
).35.
Y. J.
Hong
, S. M.
Lee
, G. C.
Kim
, and J. K.
Lee
, “Modeling high-pressure microplasmas: Comparison of fluid modeling and particle-in-cell Monte Carlo collision modeling
,” Plasma Processes Polym.
5
, 583
(2008
).36.
F.
Iza
, J.
Walsh
, and M. G.
Kong
, “From sub-microsecond to nanosecond pulsed atmospheric-pressure plasmas
,” IEEE Trans. Plasma Sci.
37
(7
), 1289
(2009
).37.
Z.
Xiong
, E.
Robert
, V.
Sarron
, J.-M.
Pouvesle
, and M. J.
Kushner
, “Dynamics of ionization wave splitting and merging of atmospheric pressure plasmas in branched dielectric tubes and channels
,” J. Phys. D
45
, 275201
(2012
).38.
Z.
Xiong
and M. J.
Kushner
, “Atmospheric pressure ionization waves propagating through a flexible capillary channel and impinging upon a target
,” Plasma Sources Sci. Technol.
21
, 034001
(2012
).39.
A.
Shashurin
, M. N.
Shneider
, A.
Dogariu
, R. B.
Miles
, and M.
Keidar
, Appl. Phys. Lett.
94
, 231504
(2009
).40.
A.
Shashurin
, M. N.
Shneider
, A.
Dogariu
, R. B.
Miles
, and M.
Keidar
, Appl. Phys. Lett.
96
, 171502
(2010
).41.
X.
Lu
and M.
Laroussi
, “Dynamics of an atmospheric pressure plasma plume generated by submicrosecond voltage pulses
,” J. Appl. Phys.
100
, 063302
(2006
).42.
B. L.
Sands
, B. N.
Ganguly
, and K.
Tachibana
, Appl. Phys. Lett.
92
, 151503
(2008
).43.
R.
Ye
and W.
Zheng
, Appl. Phys. Lett.
93
, 071502
(2008
).44.
N.
Mericam-Bourdet
, M.
Laroussi
, A.
Begum
, and E.
Karakas
, J. Phys. D: Appl. Phys.
42
, 055207
(2009
).45.
A.
Shashurin
, M. N.
Shneider
, and M.
Keidar
, “Measurements of streamer head potential and conductivity of streamer column in cold nonequilibrium atmospheric plasmas
,” Plasma Sources Sci. Technol.
21
, 034006
(2012
);
[PubMed]
A.
Shashurin
, M. N.
Shneider
, and M.
Keidar
, “Erratum
,” Plasma Sources Sci. Technol.
21
, 049601
(2012
).46.
O.
Volotskova
, A.
Shashurin
, M. A.
Stepp
, S.
Pal-Ghosh
, and M.
Keidar
, “Plasma-controlled cell migration: Localization of cold plasma-cell interaction region
,” Plasma Med.
1
, 85
(2010
).47.
M. N.
Shneider
and R. B.
Miles
, “Microwave diagnostics of small plasma objects
,” J. Appl. Phys.
98
, 033301
(2005
).48.
Z.
Zhang
, M. N.
Shneider
, and R. B.
Miles
, “Coherent microwave Rayleigh scattering from resonance-enhanced multiphoton ionization in argon
,” Phys Rev. Lett.
98
, 265005
(2007
).49.
Z.
Zhang
, M. N.
Shneider
, and R. B.
Miles
, “Microwave diagnostics of laser-induced avalanche ionization in air
,” J. Appl. Phys.
100
, 074912
(2006
).50.
Y. P.
Raizer
, G. M.
Milikh
, M. N.
Shneider
, and S. V.
Novakovski
, J. Phys. D: Appl.Phys.
31
, 3255
(1998
).51.
O.
Volotskova
, T. S.
Hawley
, M. A.
Stepp
, and M.
Keidar
, “Targeting the cancer cell cycle by cold atmospheric plasma
,” Sci. Rep.
2
, 636
(2012
).52.
D. L.
Longo
and D.
Longo
, Harrison's Hematology and Oncology
(McGraw-Hill Medical
, New York
, 2010
), pp. 294
–318
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