Swine wastewater pretreated by solid–liquid separation was optimized for biogas production and water purification. Dynamic diversity of the bacterial community in the anaerobic plug flow reactor was investigated under various temperatures and hydraulic retention times (HRT). Results of batch experiments indicated that under optimal operating conditions, biogas production, methane production rate, and chemical oxygen demand (COD) removal efficiencies increased up to 26%, 52%, and 44%, respectively. In the continuous experiments, optimal operating conditions for the plug flow operator were found to be HRT of 8 d and temperature of 35 °C, under which relatively high CH4 concentration (67%), CH4 production rate (4.6 l/d), and total COD removal (79%) were achieved. A minimum of 12 bands was observed in the denaturing gradient gel electrophoresis profile. Phylogenetic analysis revealed the predominance of phyla Firmicutes, Proteobacteria, and Euryarchaeota in the anaerobic plug-flow reactor. However, the occurrence and prevalence of specific bacterial species varied with HRT and temperature. This study establishes the optimal operating parameters for the anaerobic plug-flow digestion of swine wastewater, which can be employed in a full-scale reactor to maximize biogas production and to purify water quality.

Topics
Energy production, transmission and distribution, Methane production, Biofuels, Hydrology, Waste-water treatment, Electrophoresis, Organic compounds, Chemical oxygen demand, pH value, Fermentation process
1.
S. S.
 
Heubeck
 and
R. J. R.
 
Craggs
,
Water Sci. Technol.
 
61
,
1019
(
2010
).
https://doi.org/10.2166/wst.2010.965
Google Scholar
 
2.
Yadvika
,
Santosh
,
T. R.
 
Sreekrishnan
,
S.
 
Kohli
, and
V.
 
Rana
,
Bioresour. Technol.
 
95
,
1
(
2004
).
https://doi.org/10.1016/j.biortech.2004.02.010
Google Scholar
 
3.
S.
 
Verma
, “
Anaerobic digestion of biodegradable organics in municipal solid wastes
,” Master thesis (
Department of Earth & Environmental Engineering (Henry Krumb School of Mines) Fu Foundation School of Engineering & Applied Science, Columbia University, USA
,
2002
).
Google Scholar
 
4.
B. L. B.
 
McDermott
,
A. D. A.
 
Chalmers
, and
J. A. J.
 
Goodwin
,
Environ. Technol.
 
22
,
823
(
2001
).
https://doi.org/10.1080/095933322086180317
Google Scholar
 
5.
D. R. D.
 
Kashyap
,
K. S. K.
 
Dadhich
, and
S. K. S.
 
Sharma
,
Bioresour. Technol.
 
87
,
147
(
2003
).
https://doi.org/10.1016/S0960-8524(02)00205-5
Google Scholar
 
6.
A. K.
 
Kalia
 and
S. S.
 
Kanwar
,
Bioresour. Technol.
 
65
,
61
(
1998
).
https://doi.org/10.1016/S0960-8524(98)00021-2
Google Scholar
 
7.
E.
 
Sánchez
,
R.
 
Borja
,
P.
 
Weiland
,
L.
 
Travieso
, and
A.
 
Martín
,
Process Biochem. (Oxford, U.K.)
 
37
,
483
(
2001
).
https://doi.org/10.1016/S0032-9592(01)00240-0
Google Scholar
 
8.
K. J.
 
Chae
,
A.
 
Jang
,
S. K.
 
Yim
, and
I. S.
 
Kim
,
Bioresour. Technol.
 
99
,
1
(
2008
).
https://doi.org/10.1016/j.biortech.2006.11.063
Google Scholar
 
9.
A.
 
Converti
,
L. G.
 
Rezzani
, and
M. D.
 
Borghi
,
J. Air Waste Manage. Assoc.
 
48
,
366
(
1998
).
https://doi.org/10.1080/10473289.1998.10463689
Google Scholar
 
10.
O. O.
 
Francioso
,
M. T. M.
 
Rodriguez-Estrada
,
D. D.
 
Montecchio
,
C. C.
 
Salomoni
,
A. A.
 
Caputo
, and
D. D.
 
Palenzona
,
J. Hazard. Mater.
 
175
,
740
(
2010
).
https://doi.org/10.1016/j.jhazmat.2009.10.071
Google Scholar
 
11.
A.
 
Davidsson
,
C.
 
Lövstedt
,
J. l.
 
Jansen
,
C.
 
Gruvberger
, and
H.
 
Aspegren
,
Waste Manage.
 
28
,
986
(
2008
).
https://doi.org/10.1016/j.wasman.2007.03.024
Google Scholar
 
12.
S.
 
Luostarinen
,
S.
 
Luste
, and
M.
 
Sillanpää
,
Bioresour. Technol.
 
100
,
79
(
2009
).
https://doi.org/10.1016/j.biortech.2008.06.029
Google Scholar
 
13.
H. H.
 
Lindorfer
,
A. A.
 
Corcoba
,
V. V.
 
Vasilieva
,
R. R.
 
Braun
, and
R. R.
 
Kirchmayr
,
Bioresour. Technol.
 
99
,
1148
(
2008
).
https://doi.org/10.1016/j.biortech.2007.02.033
Google Scholar
 
14.
D. R.
 
Vartak
,
C. R.
 
Engler
,
M. J.
 
McFarland
, and
S. C.
 
Ricke
,
Bioresour. Technol.
 
62
,
79
–
84
(
1997
).
https://doi.org/10.1016/S0960-8524(97)00135-1
Google Scholar
 
15.
E. P.
 
Sánchez
,
O.
 
Monroy
,
R. O.
 
Cañizares
,
L.
 
Travieso
, and
A.
 
Ramos
,
J. Agric. Eng. Res.
 
62
,
71
(
1995
).
https://doi.org/10.1006/jaer.1995.1065
Google Scholar
 
16.
E.
 
Sánchez
,
L.
 
Travieso
,
P.
 
Weiland
,
R.
 
Borja
, and
S.
 
Nikolaeva
,
Resour. Conserv. Recycl.
 
36
,
73
(
2002
).
https://doi.org/10.1016/S0921-3449(02)00017-4
Google Scholar
 
17.
P. Y.
 
Yang
 and
C.
 
Gan
,
Bioresour. Technol.
 
65
,
21
(
1998
).
https://doi.org/10.1016/S0960-8524(98)00041-8
Google Scholar
 
18.
C.
 
Rico
,
J. L.
 
Rico
,
I.
 
Tejero
,
N.
 
Muñoz
, and
B.
 
Gómez
,
Waste Manage.
 
31
,
2167
(
2011
).
https://doi.org/10.1016/j.wasman.2011.04.018
Google Scholar
 
19.
D. -M. D.
 
Zhang
,
Y. -X. Y.
 
Chen
,
G. G.
 
Jilani
,
W. -X. W.
 
Wu
,
W. -L. W.
 
Liu
, and
Z. -Y. Z.
 
Han
,
Bioresour. Technol.
 
116
,
386
(
2012
).
https://doi.org/10.1016/j.biortech.2012.03.107
Google Scholar
 
20.
L. L.
 
Ferreira
,
E. E.
 
Duarte
, and
D. D.
 
Figueiredo
,
Bioresour. Technol.
 
116
,
285
(
2012
).
https://doi.org/10.1016/j.biortech.2012.03.063
Google Scholar
 
21.
Y. Y.
 
Xia
,
D. I. D.
 
Massé
,
T. A. T.
 
McAllister
,
C. C.
 
Beaulieu
, and
E. E.
 
Ungerfeld
,
Waste Manage.
 
32
,
404
(
2012
).
https://doi.org/10.1016/j.wasman.2011.10.024
Google Scholar
 
22.
D.
 
Hill
,
Bioresour. Technol.
 
74
,
241
(
2000
).
https://doi.org/10.1016/S0960-8524(00)00008-0
Google Scholar
 
23.
M. T.
 
Koh
 and
C. T.
 
Liaw
, in
The Environmental Center for Livestock Waste Management, Third International Symposium
(
National Pingtung University of Science and Technology
,
Pingtung, Taiwan
,
1999
), p.
281
.
24.
W. C.
 
Lin
,
Y. P.
 
Chen
, and
C. P.
 
Tseng
,
Bioresour. Technol.
 
135
,
283
(
2013
).
https://doi.org/10.1016/j.biortech.2012.10.040
Google Scholar
 
25.
R. A.
 
Sandaa
,
O.
 
Enger
, and
V.
 
Torsvik
,
Appl. Environ. Microbiol.
 
65
,
3293
(
1999
).
Google Scholar
 
26.
U.
 
Nübel
,
B.
 
Engelen
,
A.
 
Felske
,
J.
 
Snaidr
,
A.
 
Wieshuber
,
R. I.
 
Amann
,
W.
 
Ludwig
, and
H.
 
Backhaus
,
J. Bacteriol.
 
178
,
5636
(
1996
).
Google Scholar
 
27.
M. M.
 
Utsumi
,
S. E. S.
 
Belova
,
G. M. G.
 
King
, and
H. H.
 
Uchiyama
,
J. Gen. Appl. Microbiol.
 
49
,
75
(
2003
).
https://doi.org/10.2323/jgam.49.75
Google Scholar
 
28.
APHA
,
Standard Methods for the Examination of Water & Wastewater, Centennial Edition
, 21st ed. (
American Public Health Association
,
2005
).
29.
M.
 
Khemis
,
G.
 
Tanguy
,
J. P.
 
Leclerc
,
G.
 
Valentin
, and
F.
 
Lapicque
,
Process Saf. Environ. Prot.
 
83
,
50
(
2005
).
https://doi.org/10.1205/psep.03381
Google Scholar
 
30.
S.
 
McHugh
,
G.
 
Collins
, and
V.
 
O’Flaherty
,
Bioresour. Technol.
 
97
,
1669
(
2006
).
https://doi.org/10.1016/j.biortech.2005.07.020
Google Scholar
 
31.
O.
 
Lahav
 and
R. E.
 
Loewenthal
,
Water SA
 
26
,
389
(
2000
).
Google Scholar
 
32.
E.
 
Sánchez
,
R.
 
Borja
,
L.
 
Travieso
,
A.
 
Martín
, and
M. F.
 
Colmenarejo
,
Bioresour. Technol.
 
96
,
335
(
2005
).
https://doi.org/10.1016/j.biortech.2004.04.003
Google Scholar
 
33.
Y.
 
Park
,
F.
 
Hong
,
J.
 
Cheon
,
T.
 
Hidaka
, and
H.
 
Tsuno
,
J. Biosci. Bioeng.
 
105
,
48
(
2008
).
https://doi.org/10.1263/jbb.105.48
Google Scholar
 
34.
K.
 
Komemoto
,
Y. G.
 
Lim
,
N.
 
Nagao
,
Y.
 
Onoue
,
C.
 
Niwa
, and
T.
 
Toda
,
Waste Manage.
 
29
,
2950
(
2009
).
https://doi.org/10.1016/j.wasman.2009.07.011
Google Scholar
 
35.
W. S.
 
Kissalita
,
K. V.
 
Lo
, and
K. L.
 
Pinder
,
Biotechnol. Bioeng.
 
33
,
623
(
1989
).
https://doi.org/10.1002/bit.260330516
Google Scholar
 
36.
J.
 
Cardinali-Rezende
,
R. B.
 
Debarry
,
L. F. D. B.
 
Colturato
,
E. V.
 
Carneiro
,
E.
 
Chartone-Souza
, and
A. M. A.
 
Nascimento
,
Appl. Microbiol. Biotechnol.
 
84
,
777
(
2009
).
https://doi.org/10.1007/s00253-009-2071-z
Google Scholar
 
37.
H.
 
Shiratori
,
H.
 
Ikeno
,
S.
 
Ayame
,
N.
 
Kataoka
,
A.
 
Miya
,
K.
 
Hosono
,
T.
 
Beppu
, and
K.
 
Ueda
,
Appl. Environ. Microbiol.
 
72
,
3702
(
2006
).
https://doi.org/10.1128/AEM.72.5.3702-3709.2006
Google Scholar
 
38.
C.
 
Ash
,
F. G.
 
Priest
, and
M. D.
 
Collins
,
Antonie van Leeuwenhoek
 
64
,
253
(
1993
).
https://doi.org/10.1007/BF00873085
Google Scholar
 
39.
H. J.
 
Nanninga
 and
J. C.
 
Gottschal
,
Appl. Environ. Microbiol.
 
53
,
802
(
1987
).
Google Scholar
 
40.
J. P.
 
Touzel
,
G.
 
Prensier
,
J. L.
 
Roustan
,
I.
 
Thomas
,
H. C.
 
Dubourguier
, and
G.
 
Albagnac
,
Int. J. Syst. Bacteriol.
 
38
,
30
(
1988
).
https://doi.org/10.1099/00207713-38-1-30
Google Scholar
 
41.
H.
 
Cadillo-Quiroz
,
J. B.
 
Yavitt
, and
S. H.
 
Zinder
,
Int. J. Sys. Evol. Microbiol.
 
59
,
928
(
2009
).
https://doi.org/10.1099/ijs.0.006890-0
Google Scholar
 
42.
H.
 
Imachi
,
S.
 
Sakai
,
Y.
 
Sekiguchi
,
S.
 
Hanada
,
Y.
 
Kamagata
,
A.
 
Ohashi
, and
H.
 
Harada
,
Int. J. Sys. Evol. Microbiol.
 
58
,
294
(
2007
).
https://doi.org/10.1099/ijs.0.65394-0
Google Scholar
 
43.
K.
 
Syutsubo
,
Y.
 
Nagaya
,
S.
 
Sakai
, and
A.
 
Miya
,
Water Sci. Technol.
 
52
,
79
(
2005
).
Google Scholar
 
44.
Y.
 
Yashiro
,
S.
 
Sakai
,
M.
 
Ehara
,
M.
 
Miyazaki
,
T.
 
Yamaguchi
, and
H.
 
Imachi
,
Int. J. Sys. Evol. Microbiol.
 
61
,
53
(
2011
).
https://doi.org/10.1099/ijs.0.014811-0
Google Scholar
 
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